The DOI matches its Crossref record; 102 of the 102 references it cites were found in a registry or an index.
Site-Specific Antibody–Drug Conjugates: The Nexus of Bioorthogonal Chemistry, Protein Engineering, and Drug Development
Open accessAbstract
Antibody-drug conjugates (ADCs) combine the specificity of antibodies with the potency of small molecules to create targeted drugs. Despite the simplicity of this concept, generation of clinically successful ADCs has been very difficult. Over the past several decades, scientists have learned a great deal about the constraints on antibodies, linkers, and drugs as they relate to successful construction of ADCs. Once these components are in hand, most ADCs are prepared by nonspecific modification of antibody lysine or cysteine residues with drug-linker reagents, which results in heterogeneous product mixtures that cannot be further purified. With advances in the fields of bioorthogonal chemistry and protein engineering, there is growing interest in producing ADCs by site-specific conjugation to the antibody, yielding more homogeneous products that have demonstrated benefits over their heterogeneous counterparts in vivo. Here, we chronicle the development of a multitude of site-specific conjugation strategies for assembly of ADCs and provide a comprehensive account of key advances and their roots in the fields of bioorthogonal chemistry and protein engineering.
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Cites
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Cited by
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Antibody drug conjugate: the “biological missile” for targeted cancer therapy
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Transport of drugs from blood vessels to tumour tissue
Dewhirst, M. W., & Secomb, T. W. (2017) · Nature Reviews Cancer · doi:10.1038/nrc.2017.93 · Cited by 749
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Redox-based reagents for chemoselective methionine bioconjugation
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Metadata
- Authors
- Agarwal, P., & Bertozzi, C. R.
- Venue
- Bioconjugate Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2015-01-30
- Volume
- 26
- Issue
- 2
- Pages
- 176-192
- ISSN
- 1043-1802 (ISSN-L)
- 1520-4812
- OpenAlex
- W1984111577
- Cited by
- 605
- References
- 102
- FWCI
- 36.80
- Type
- journal article
- Language
- en
- Open access
- hybrid
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