The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Electrochemistry of Methylene Blue Bound to a DNA-Modified Electrode
Abstract
Gold surfaces have been derivatized with 15-base-pair double-stranded DNA oligonucleotides containing a pendant 5' hexanethiol linker. The electrochemistry of intercalated methylene blue has been investigated at these modified electrodes. Chronocoulometry, cyclic voltammetry, ellipsometry, and quantitation via 32P labeling are all consistent with a surface coverage of > or = 75% with the DNA helices stacked at an angle from the electrode surface. Cyclic voltammetry at low methylene blue/ duplex stoichiometries yields well-behaved surface waves with E degrees = -0.25 V (vs SCE), a value 0.03 V negative of that in aqueous solution. A binding isotherm for methylene blue at an electrode derivatized with the double-stranded sequence 5' SH-(CH2)6-p-AGTACAGTCATCGCG 3' was obtained from coulometric titrations and gave an affinity constant equal to 3.8(5) x 10(6) M-1 with a saturation value of 1.4(2) methylene blue intercalators per DNA duplex. Taken together, these experiments support a model for the surface morphology in which DNA duplexes are densely packed; methylene blue therefore reversibly binds to sites in the DNA that are close to the bulk solution. Electrochemistry at DNA-derivatized electrodes provides a valuable methodology to examine DNA-bound redox reactions and may offer new insight into DNA-mediated electron transfers.
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Citations
Cites
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Self-citationOxidative DNA damage through long-range electron transfer
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Cited by
Drummond, T. G., Hill, M. G., & Barton, J. K. (2003) · Nature Biotechnology · doi:10.1038/nbt873 · Cited by 2,214
Self-citationDNA Biosensors and Microarrays
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Non-Platinum Chemotherapeutic Metallopharmaceuticals
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Self-citationSaunders, S. H., Tse, E. C. M., Yates, M. D., Otero, F. J., Trammell, S. A., Stemp, E. D. A., Barton, J. K., Tender, L. M., & Newman, D. K. (2020) · Cell · doi:10.1016/j.cell.2020.07.006 · Cited by 328
Self-citationDirect measurement of electrical transport through single DNA molecules of complex sequence
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Metadata
- Authors
- Kelley, S. O., Barton, J. K., Jackson, N. M., & Hill, M. G.
- Venue
- Bioconjugate Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1997-01-01
- Volume
- 8
- Issue
- 1
- Pages
- 31-37
- ISSN
- 1043-1802 (ISSN-L)
- 1520-4812
- OpenAlex
- W2141429657
- Cited by
- 481
- References
- 30
- FWCI
- 3.71
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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