The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Substrate specificity of the Escherichia coli endonuclease III: Excision of thymine- and cytosine-derived lesions in DNA produced by radiation-generated free radicals
Abstract
The excision of modified bases from DNA by Escherichia coli endonuclease III was investigated. Modified bases were produced in DNA by exposure of dilute buffered solutions of DNA to ionizing radiation under oxic or anoxic conditions. The technique of gas chromatography/mass spectrometry (GC/MS) was used to identify and quantify 16 pyrimidine- and purine-derived DNA lesions. DNA substrates were incubated either with the native enzyme or with the heat-inactivated enzyme. Subsequently, DNA was precipitated. Pellets were analyzed by GC/MS after hydrolysis and derivatization. Supernatant fractions were analyzed after derivatization without hydrolysis. The results provided unequivocal evidence for the excision by E. coli endonuclease III of a number of thymine- and cytosine-derived lesions from DNA. These were 5,6-dihydrothymine, 5-hydroxy-5-methylhydantoin, thymine glycol, 5-hydroxy-6-hydrothymine, 5,6-dihydrouracil, alloxan, uracil glycol, and 5-hydroxy-6-hydrouracil. None of the purine-derived lesions was excised by endonuclease III. The present work extends the substrate specificity of E. coli endonuclease III to another thymine-derived and four cytosine-derived lesions. It is the first investigation of the substrate specificity of this repair enzyme in the context of a large number of pyrimidine- and purine-derived lesions in DNA.
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Citations
Cites
Instability and decay of the primary structure of DNA
Lindahl, T. (1993) · Nature · doi:10.1038/362709a0 · Cited by 5,679
Modification of DNA bases in mammalian chromatin by radiation-generated free radicals
Gajewski, E., Rao, G., Nackerdien, Z., & Dizdaroglu, M. (1990) · Biochemistry · doi:10.1021/bi00486a014 · Cited by 258
Cited by
Oxidative DNA damage: mechanisms, mutation, and disease
Cooke, M. S., Evans, M. D., Dizdaroglu, M., & Lunec, J. (2003) · The FASEB Journal · doi:10.1096/fj.02-0752rev · Cited by 3,245
WISEMAN, H., & HALLIWELL, B. (1996) · Biochemical Journal · doi:10.1042/bj3130017 · Cited by 2,345
Oxidative DNA damage and disease: induction, repair and significance
Evans, M. D., Dizdaroglu, M., & Cooke, M. S. (2004) · Mutation Research/Reviews in Mutation Research · doi:10.1016/j.mrrev.2003.11.001 · Cited by 1,372
Free radical-induced damage to DNA: mechanisms and measurement
Dizdaroglu, M., Jaruga, P., Birincioglu, M., & Rodriguez, H. (2002) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(02)00826-2 · Cited by 953
Self-citationMutagenicity and repair of oxidative DNA damage: insights from studies using defined lesions
Wang, D., Kreutzer, D. A., & Essigmann, J. M. (1998) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/s0027-5107(98)00066-9 · Cited by 464
Metadata
- Authors
- Dizdaroglu, M., Laval, J., & Boiteux, S.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1993-11-01
- Volume
- 32
- Issue
- 45
- Pages
- 12105-12111
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2080733065
- Cited by
- 283
- References
- 2
- FWCI
- 3.62
- Type
- journal article
- Language
- en
- Open access
- closed
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- Dizdaroglu, M., Laval, J., & Boiteux, S. (1993). Substrate specificity of the Escherichia coli endonuclease III: Excision of thymine- and cytosine-derived lesions in DNA produced by radiation-generated free radicals. Biochemistry. https://doi.org/10.1021/bi00096a022
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