The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Determination of kinetic constants for peptidyl prolyl cis-trans isomerases by an improved spectrophotometric assay
Abstract
The kinetic properties and substrate specificity of two well-characterized peptidyl prolyl cis-trans isomerases (PPIases), cyclophilin and the FK-506 binding protein (FKBP), have been previously examined [Fischer, G., Bang, H., Berger, E., & Schellenberger, A. (1984) Biochim. Biophys. Acta 791, 87-97; Harrison, R.K., & Stein, R.L. (1990) Biochemistry 29, 1684-1689; Albers, M.W., Walsh, C.T., & Schreiber, S. L. (1990) J. Org. Chem. 55, 4984-4986]. The chymotrypsin-coupled enzymatic assay employed in these studies suffers from two serious shortcomings. Due to the low equilibrium population of the X-cis-Pro-Phe-pNA isomer (the PPIase substrate), in conjunction with the low solubility of p-nitroaniline generated by chymotrypsin hydrolysis, substrate concentrations in the saturating region are not experimentally attainable. Secondly, the uncatalyzed cis-trans isomerization obscures the interpretation of the initial velocity. As a result of these limitations, the steady-state kinetic parameters (Km,Kcat) have not been determined. Here we introduce an improved version of the spectrophotometric assay and report for the first time the Michaelis constants and turnover numbers for both PPIases with established substrates. The improvements in the experimental conditions originate in a medium-induced increase in the equilibrium population of the cis X-Pro conformer and in conducting the assay at 0 degrees C to suppress the uncatalyzed thermal isomerization. In addition, we present a rigorous mathematical model of the spectrophotometric progress curves that accounts for the contributions of the residual background rate.(ABSTRACT TRUNCATED AT 250 WORDS)
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Citations
Cites
An Algorithm for Least-Squares Estimation of Nonlinear Parameters
Marquardt, D. W. (1963) · Journal of the Society for Industrial and Applied Mathematics · doi:10.1137/0111030 · Cited by 30,728
Cited by
Ranganathan, R., Lu, K. P., Hunter, T., & Noel, J. P. (1997) · Cell · doi:10.1016/s0092-8674(00)80273-1 · Cited by 699
Active Site Residues of Cyclophilin A Are Crucial for Its Signaling Activity via CD147
Yurchenko, V., Zybarth, G., O'Connor, M., Dai, W. W., Franchin, G., Hao, T., Guo, H., Hung, H.-C., Toole, B., Gallay, P., et al. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m201593200 · Cited by 334
Mitochondrial Targeted Cyclophilin D Protects Cells from Cell Death by Peptidyl Prolyl Isomerization
Lin, D.-T., & Lechleiter, J. D. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m112035200 · Cited by 181
Fluorescent Probes to Characterise FK506‐Binding Proteins
Kozany, C., März, A., Kress, C., & Hausch, F. (2009) · ChemBioChem · doi:10.1002/cbic.200800806 · Cited by 91
Targeting FKBP isoforms with small-molecule ligands
Blackburn, E. A., & Walkinshaw, M. D. (2011) · Current Opinion in Pharmacology · doi:10.1016/j.coph.2011.04.007 · Cited by 73
Metadata
- Authors
- Kofron, J. L., Kuzmic, P., Kishore, V., Colon-Bonilla, E., & Rich, D. H.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1991-06-25
- Volume
- 30
- Issue
- 25
- Pages
- 6127-6134
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2109188826
- Cited by
- 507
- References
- 1
- FWCI
- 7.10
- Type
- journal article
- Language
- en
- Open access
- closed
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