Purification and characterization of bovine hepatic uroporphyrinogen decarboxylase
Abstract
Uroporphyrinogen decarboxylase (EC 4.1.1.37) has been purified to homogeneity from bovine liver by using isoelectric and salt precipitations, followed by chromatography on DEAE-cellulose, phenyl-Sepharose, hydroxylapatite, and Sephacryl S-200. The purified enzyme is a monomer with an Mr approximately 57 000 and an isoelectric point at pH 4.6. Enzyme activity is optimal in buffers having an ionic strength of approximately 0.1 M and a pH of 6.8. The purified enzyme has a specific activity (expressed as the disappearance of uroporphyrinogen I) of 936 nmol X h-1 X (mg of protein)-1. The purified enzyme catalyzes all four decarboxylation reactions in the conversion of uroporphyrinogen I or III to the corresponding coproporphyrinogen. The rate-limiting step in the physiologically significant conversion of uroporphyrinogen III to coproporphyrinogen III is the decarboxylation of heptacarboxylate III. Kinetic data suggest that the enzyme has at least two noninteracting active sites. At least one sulfhydryl group is required for catalytic activity. The enzyme is inhibited by sulfhydryl-specific reagents and by divalent metal ions including Fe2+, Co2+, Cu2+, Zn2+, and Pb2+. The pattern of accumulation of intermediate (hepta-, hexa-, and pentacarboxylate porphyrinogens) and final (coproporphyrinogen) decarboxylation products is affected by the ratio of substrate (uroporphyrinogen I or III) concentration to enzyme concentration. Under physiologic conditions where the uroporphyrinogen to enzyme ratio is low, the substrate is nearly quantitatively decarboxylated, and the major product is coproporphyrinogen. If the ratio of uroporphyrinogen to enzyme is high, intermediates accumulate, and heptacarboxylate porphyrinogen becomes the major decarboxylation product.(ABSTRACT TRUNCATED AT 250 WORDS)
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Uroporphyrinogen decarboxylase (UROD) catalyzes decarboxylation of all four acetate side chains of uroporphyrinogen III to methyl groups.
“Uroporphyrinogen decarboxylase (UROD) catalyzes decarboxylation of all four acetate side chains of uroporphyrinogen III to methyl groups [17].”
From Aminolevulinic Acid (ALA) as a Prodrug in Photodynamic Therapy of Cancer (2011) · cites this paper as [17]
Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O. H., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 315,771 · see citing works
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302 · see citing works
High resolution two-dimensional electrophoresis of proteins.
O’Farrell, P. H. (1975) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)41496-8 · Cited by 19,248 · see citing works
Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane
Fairbanks, G., Steck, T. L., & Wallach, D. F. H. (1971) · Biochemistry · doi:10.1021/bi00789a030 · Cited by 8,526 · see citing works
Hedrick, J. L., & Smith, A. J. (1968) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(68)90569-9 · Cited by 1,901 · see citing works
Reisner, A. H., Nemes, P., & Bucholtz, C. A. (1975) · Analytical Biochemistry · doi:10.1016/0003-2697(75)90461-3 · Cited by 618 · see citing works
Atkinson, D. (1966) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.35.070166.000505 · Cited by 614 · see citing works
[39] Analytical disc gel electrophoresis
Gabriel, O. (1971) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(71)22041-3 · Cited by 493 · see citing works
Kushner, J. P., Barbuto, A. J., & Lee, G. R. (1976) · Journal of Clinical Investigation · doi:10.1172/jci108560 · Cited by 277 · see citing works
Self-citationDecreased Activity of Hepatic Uroporphyrinogen Decarboxylase in Sporadic Porphyria Cutanea Tarda
Elder, G. H., Lee, G. B., & Tovey, J. A. (1978) · New England Journal of Medicine · doi:10.1056/nejm197808102990603 · Cited by 203 · see citing works
Enzymic parameters: Measurement of V and Km
Lee, H.-J., & Wilson, I. B. (1971) · Biochimica et Biophysica Acta (BBA) - Enzymology · doi:10.1016/0005-2744(71)90144-6 · Cited by 187 · see citing works
Macrocyclic intermediates in the biosynthesis of porphyrins
JACKSON, A. H., Sancovich, H. A., Ferramola, A. M., Evans, N., Games, D. E., Matlin, S. A., Elder, G. H., & Smith, S. G. (1976) · Philosophical transactions of the Royal Society of London. Series B, Biological sciences · doi:10.1098/rstb.1976.0009 · Cited by 140 · see citing works
de Verneuil, H., Sassa, S., & Kappas, A. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)32947-8 · Cited by 139 · see citing works
Porphyria Cutanea Tarda in Three Generations of a Single Family
Benedetto, A. V., Kushner, J. P., & Taylor, J. S. (1978) · New England Journal of Medicine · doi:10.1056/nejm197802162980702 · Cited by 86 · see citing works
Self-citationDecreased Hepatic Uroporphyrinogen Decarboxylase Activity in Porphyria Cutanea Tarda
Felsher, B. F., Carpio, N. M., Engleking, D. W., & Nunn, A. T. (1982) · New England Journal of Medicine · doi:10.1056/nejm198204013061302 · Cited by 79 · see citing works
Some kinetic properties of human red cell uroporphyrinogen decarboxylase
de Verneuil, H., Grandchamp, B., & NORDMANN, Y. (1980) · Biochimica et Biophysica Acta (BBA) - Enzymology · doi:10.1016/0005-2744(80)90053-4 · Cited by 65 · see citing works
Uroporphyrinogen decarboxylase from mouse spleen
Romeo, G., & Levin, E. Y. (1971) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/0304-4165(71)90220-0 · Cited by 60 · see citing works
Felsher, B. F., Norris, M., & Shih, J. C. (1978) · New England Journal of Medicine · doi:10.1056/nejm197811162992002 · Cited by 58 · see citing works
Tomio, J. M., Garcia, R. C., de Viale, L. C. S. M., & Grinstein, M. (1970) · Biochimica et Biophysica Acta (BBA) - Enzymology · doi:10.1016/0005-2744(70)90068-9 · Cited by 57 · see citing works
Decarboxylation of porphyrinogens by rat liver uroporphyrinogen decarboxylase
Smith, A. G., & Francis, J. E. (1979) · Biochemical Journal · doi:10.1042/bj1830455 · Cited by 55 · see citing works
Hepatic siderosis and porphyria cutanea tarda: relation of iron excess to the metabolic defect.
Bf, F., & Jp, K. (1977) · PubMed · Cited by 45 · see citing works
Familial and sporadic porphyria cutanea: two different diseases
de Verneuil, H., NORDMANN, Y., Phung, N., Grandchamp, B., Aïtken, G., Grelier, M., & Noiré, J. (1978) · International Journal of Biochemistry · doi:10.1016/0020-711x(78)90072-1 · Cited by 35 · see citing works
Histidine decarboxylase of Lactobacillus 30a. III. Composition and subunit structure
Chang, G. W., & Snell, E. E. (1968) · Biochemistry · doi:10.1021/bi00846a002 · Cited by 27 · see citing works
Formation of adenosine triphosphate in the oxidation of a model for the reduced pyridine nucleotides
Bechara, E. J. H., & Cilento, G. (1972) · Biochemistry · doi:10.1021/bi00764a009 · Cited by 10 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Biosynthesis of heme in mammals
Ajioka, R. S., Phillips, J. D., & Kushner, J. P. (2006) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2006.05.005 · Cited by 493 · see citing works
Self-citationAminolevulinic Acid (ALA) as a Prodrug in Photodynamic Therapy of Cancer
Wachowska, M., Muchowicz, A., Firczuk, M., Gabrysiak, M., Winiarska, M., Wańczyk, M., Bojarczuk, K., & Golab, J. (2011) · Molecules · doi:10.3390/molecules16054140 · Cited by 265 · see citing works
A porphomethene inhibitor of uroporphyrinogen decarboxylase causes porphyria cutanea tarda
Phillips, J. D., Bergonia, H. A., Reilly, C. A., Franklin, M. R., & Kushner, J. P. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0700547104 · Cited by 156 · see citing works
Self-citationMolecular cloning and nucleotide sequence of a complete human uroporphyrinogen decarboxylase cDNA.
Roméo, P., Raich, N., Dubart‐Kupperschmitt, A., Beaupain, D., Pryor, M., Kushner, J. P., Cohen-Solal, M., & Goossens, M. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)67589-1 · Cited by 107 · see citing works
Self-citationKwon, S., de Boer, A. L., Petri, R., & Schmidt‐Dannert, C. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.8.4875-4883.2003 · Cited by 85 · see citing works
Structural basis for tetrapyrrole coordination by uroporphyrinogen decarboxylase
Phillips, J. D., Whitby, F. G., Kushner, J. P., & Hill, C. P. (2003) · The EMBO Journal · doi:10.1093/emboj/cdg606 · Cited by 83 · see citing works
Self-citationUroporphyrinogen decarboxylase
Elder, G. H., & Roberts, A. (1995) · Journal of Bioenergetics and Biomembranes · doi:10.1007/bf02110035 · Cited by 76 · see citing works
Mukerji, S. K., Pimstone, N. R., & Burns, M. (1984) · Gastroenterology · doi:10.1016/0016-5085(84)90189-6 · Cited by 75 · see citing works
High-performance liquid chromatography of porphyrins
Lim, C. K., Li, F., & Peters, T. J. (1988) · Journal of Chromatography B Biomedical Sciences and Applications · doi:10.1016/s0378-4347(00)83869-4 · Cited by 73 · see citing works
Kushner, J. P., Edwards, C. Q., Dadone, M. M., & Skolnick, M. H. (1985) · Gastroenterology · doi:10.1016/s0016-5085(85)80084-6 · Cited by 66 · see citing works
Self-citationGarey, J. R., Harrison, L. M., Franklin, K. F., Metcalf, K. M., Radisky, E. S., & Kushner, J. P. (1990) · Journal of Clinical Investigation · doi:10.1172/jci114856 · Cited by 65 · see citing works
Self-citationGederaas, O. A., Holroyd, A., Brown, S. B., Vernon, D. I., Moan, J. E., & Berg, K. (2001) · Photochemistry and Photobiology · doi:10.1562/0031-8655(2001)0730164aameto2.0.co2 · Cited by 62 · see citing works
Martins, B. M., Grimm, B., Mock, H., Huber, R., & Messerschmidt, A. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m104759200 · Cited by 53 · see citing works
Mock, H., Trainotti, L., Kruse, E. M., & Grimm, B. (1995) · Plant Molecular Biology · doi:10.1007/bf00020244 · Cited by 51 · see citing works
Siersema, P. D., Rademakers, L. H. P. M., Cleton, M. I., ten Kate, F. J. W., de Bruijn, W. C., Marx, J. J. M., & Wilson, J. H. P. (1995) · Journal of Hepatology · doi:10.1016/s0168-8278(95)80004-2 · Cited by 51 · see citing works
Hematologic and hepatic manifestations of the cutaneous porphyrias
Pimstone, N. R. (1985) · Clinics in Dermatology · doi:10.1016/0738-081x(85)90035-5 · Cited by 49 · see citing works
Synthesis, delivery and regulation of eukaryotic heme and Fe–S cluster cofactors
Barupala, D., Dzul, S. P., Riggs-Gelasco, P. J., & Stemmler, T. L. (2016) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2016.01.010 · Cited by 46 · see citing works
Porphyria Cutanea Tarda and Related Disorders
Elder, G. H. (2003) · Elsevier eBooks · doi:10.1016/b978-0-08-092388-8.50010-8 · Cited by 46 · see citing works
Characterization and crystallization of human uroporphyrinogen decarboxylase
Phillips, J. D., Kushner, J. P., Whitby, F. G., & Hill, C. P. (1997) · Protein Science · doi:10.1002/pro.5560060624 · Cited by 43 · see citing works
Self-citationNagaraj, V. A., Rajavelu, A., Chandra, N. R., Dasari, P., Rangarajan, P. N., & Padmanaban, G. (2008) · International Journal for Parasitology · doi:10.1016/j.ijpara.2008.10.011 · Cited by 42 · see citing works
Purification and properties of the uroporphyrinogen decarboxylase from Rhodobacter sphaeroides
Jones, R. M. H., & Jordan, P. M. (1993) · Biochemical Journal · doi:10.1042/bj2930703 · Cited by 41 · see citing works
Purification and properties of uroporphyrinogen decarboxylase from Saccharomyces cerevisiae
Félix, F., & Brouillet, N. (1990) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1990.tb15416.x · Cited by 36 · see citing works
Mylchreest, E., & Charbonneau, M. (1997) · Toxicology and Applied Pharmacology · doi:10.1006/taap.1997.8157 · Cited by 34 · see citing works
de Verneuil, H., Bourgeois, F., de Rooij, F., Siersema, P., Wilson, J. H. P., Grandchamp, B., & NORDMANN, Y. (1992) · Human Genetics · doi:10.1007/bf00219182 · Cited by 31 · see citing works
Uroporphyrinogen decarboxylase in Saccharomyces cerevisiae
Garey, J. R., Labbe‐Bois, R., Chełstowska, A., Rytka, J., Harrison, L. M., Kushner, J. P., & Labbé, P. (1992) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1992.tb16868.x · Cited by 28 · see citing works
Self-citationde Verneuil, H., Grandchamp, B., Roméo, P., Raich, N., Beaumont, C., Goossens, M., Nicolas, H., & NORDMANN, Y. (1986) · Journal of Clinical Investigation · doi:10.1172/jci112321 · Cited by 26 · see citing works
Biosynthesis of Bacteriochlorophylls in Purple Bacteria
Willows, R. D., & Kriegel, A. M. (2008) · Advances in photosynthesis and respiration · doi:10.1007/978-1-4020-8815-5_4 · Cited by 25 · see citing works
Crystal Structure of Uroporphyrinogen Decarboxylase fromBacillus subtilis
Fan, J., Liu, Q., Hao, Q., Teng, M., & Niu, L. (2006) · Journal of Bacteriology · doi:10.1128/jb.01083-06 · Cited by 24 · see citing works
Purification and properties of uroporphyrinogen decarboxylase from human erythrocytes
Roberts, A., & Elder, G. H. (1997) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(97)81042-7 · Cited by 24 · see citing works
Mukerji, S. K., & Pimstone, N. R. (1986) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(86)90630-2 · Cited by 23 · see citing works
Metal Alteration of Uroporphyrinogen Decarboxylase and Coproporphyrinogen Oxidasea
Woods, J. S., & Fowler, B. A. (1987) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1987.tb48761.x · Cited by 21 · see citing works
Mutational analysis of human uroporphyrinogen decarboxylase
Wyckoff, E. E., Phillips, J. D., Sowa, A. M., Franklin, M. R., & Kushner, J. P. (1996) · Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology · doi:10.1016/s0167-4838(96)00148-3 · Cited by 20 · see citing works
Self-citationde Catabbi, S. C. B., de Molina, M. D. C. R., & de Viale, L. C. S. M. (1991) · International Journal of Biochemistry · doi:10.1016/0020-711x(91)90037-n · Cited by 19 · see citing works
Akhtar, M. (1991) · New comprehensive biochemistry · doi:10.1016/s0167-7306(08)60109-x · Cited by 18 · see citing works
Koopmann, G. E., de Geralnik, A. A. J., & Batlle, A. M. D. C. (1986) · International Journal of Biochemistry · doi:10.1016/0020-711x(86)90075-3 · Cited by 18 · see citing works
Lash, T. D., Mani, U. N., Lyons, E. A., Thientanavanich, P., & Jones, M. A. (1998) · The Journal of Organic Chemistry · doi:10.1021/jo9814748 · Cited by 17 · see citing works
Measurement of Uroporphyrinogen Decarboxylase Activity
Phillips, J. D., & Kushner, J. P. (1999) · Current Protocols in Toxicology · doi:10.1002/0471140856.tx0804s00 · Cited by 17 · see citing works
Self-citationKurlandzka, A., Żołądek, T., Rytka, J., Labbe‐Bois, R., & Urban‐Grimal, D. (1988) · Biochemical Journal · doi:10.1042/bj2530109 · Cited by 17 · see citing works
Bushnell, E. A. C., Erdtman, E., Llano, J., Eriksson, L. A., & Gauld, J. W. (2010) · Journal of Computational Chemistry · doi:10.1002/jcc.21661 · Cited by 16 · see citing works
Chełstowska, A., Żołądek, T., Garey, J. R., Kushner, J. P., Rytka, J., & Labbe‐Bois, R. (1992) · Biochemical Journal · doi:10.1042/bj2880753 · Cited by 16 · see citing works
Self-citationA potential biochemical explanation for the genesis of porphyria cutanea tarda
Mukerji, S. K., Pimstone, N. R., & Tan, K. T. (1985) · FEBS Letters · doi:10.1016/0014-5793(85)81026-7 · Cited by 16 · see citing works
How does hexachlorobenzene treatment affect liver uroporphyrinogen decarboxylase?
Chaufan, G. (2001) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/s1357-2725(01)00034-6 · Cited by 15 · see citing works
Liver in disorders of porphyrin metabolism
Rank, J. M., Straka, J. G., & Bloomer, J. R. (1990) · Journal of Gastroenterology and Hepatology · doi:10.1111/j.1440-1746.1990.tb01443.x · Cited by 15 · see citing works
Self-citationKiel, J. A. K. W., ten Berge, A. M., & Venema, G. (1992) · DNA sequence · doi:10.3109/10425179209020822 · Cited by 15 · see citing works
Iron stimulation of free radical-mediated porphyrinogen oxidation by hepatic and renal mitochondria
Woods, J. S., & Calas, C. A. (1989) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(89)91626-4 · Cited by 14 · see citing works
Koopmann, G. E., & Batlle, A. M. D. C. (1987) · International Journal of Biochemistry · doi:10.1016/0020-711x(87)90011-5 · Cited by 14 · see citing works
Abraham, N. G., Camadro, J., Hoffstein, S. T., & Levere, R. D. (1986) · Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology · doi:10.1016/0167-4838(86)90238-4 · Cited by 13 · see citing works
Luo, J., & Lim, C. K. (1990) · Biochemical Journal · doi:10.1042/bj2680513 · Cited by 13 · see citing works
Studies on uroporphyrinogen decarboxylase of etiolated Euglena gracilis Z
Juknat, A. A., SEUBERT, A., Seubert, S., & Ippen, H. (1989) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1989.tb14570.x · Cited by 13 · see citing works
Koss, G., Losekam, M., Seidel, J., STEINBACH, K., & Koransky, W. (1987) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1987.tb48769.x · Cited by 12 · see citing works
[40] High-performance liquid chromatography of porphyrin methyl esters
Straka, J. G. (1986) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(86)23042-6 · Cited by 12 · see citing works
Self-citationFlumeri, C. D., Larocque, R., & Keng, T. (1993) · Yeast · doi:10.1002/yea.320090608 · Cited by 10 · see citing works
Koss, G., Losekam, M., Schüler, E., & Schreiber, I. M. (1991) · Comparative Biochemistry and Physiology Part B Comparative Biochemistry · doi:10.1016/0305-0491(91)90037-e · Cited by 9 · see citing works
Comparison between crab hepatopancreas and rat liver uroporphyrinogen decarboxylase
Chaufan, G. (2002) · Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology · doi:10.1016/s1096-4959(02)00151-3 · Cited by 7 · see citing works
Quintanilla‐Vega, B., Hernandez, A., & Mendoza‐Figueroa, T. (1996) · Toxicology in Vitro · doi:10.1016/s0887-2333(96)00043-4 · Cited by 7 · see citing works
Biochemistry and Molecular Biology of Chlorophyll Synthesis
Kannangara, C. G. (1991) · Elsevier eBooks · doi:10.1016/b978-0-12-715010-9.50015-3 · Cited by 7 · see citing works
Martins, B. M., Grimm, B., Mock, H., Richter, G., Huber, R., & Messerschmidt, A. (2001) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444901013361 · Cited by 6 · see citing works
Studies on uroporphyrinogen decarboxylase fromChlorella kessleri(Trebouxiophyceae, Chlorophyta)
Juárez, Á. B., Aldonatti, C. A., Vigna, M. S., & de Molina, M. D. C. R. (2007) · Canadian Journal of Microbiology · doi:10.1139/w06-117 · Cited by 5 · see citing works
Determination of Porphyrins in Physiological Samples
Ho, J. (1990) · Journal of Liquid Chromatography · doi:10.1080/01483919008049567 · Cited by 5 · see citing works
Chaufan, G., Corvi, M. M., de Viale, L. C. S. M., Cárdenas, M. L., & de Molina, M. D. C. R. (2005) · Journal of Biochemical and Molecular Toxicology · doi:10.1002/jbt.20055 · Cited by 5 · see citing works
de Catabbi, S. C. B., & de Viale, L. C. S. M. (1994) · International Journal of Biochemistry · doi:10.1016/0020-711x(94)90019-1 · Cited by 4 · see citing works
Navone, N. M., Afonso, S. G., Polo, C. F., & Battle, A. (1992) · Comparative Biochemistry and Physiology Part B Comparative Biochemistry · doi:10.1016/0305-0491(92)90277-x · Cited by 4 · see citing works
de Molina, M. D. C. R., de Calmanovici, R. W., & de Viale, L. C. S. M. (1987) · International Journal of Biochemistry · doi:10.1016/0020-711x(87)90010-3 · Cited by 4 · see citing works
Laterrière, M., d’Estaintot, B. L., Dautant, A., PRECIGOUX, G., Hombrados, I., & de Verneuil, H. (1998) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s090744499701250x · Cited by 2 · see citing works
The Biosynthesis of Hemes, Siroheme, Vitamin B12 and Linear Tetrapyrroles in Pseudomonads
Frankenberg‐Dinkel, N., Schobert, M., Moser, J., Raux, E., Graham, R. M., Warren, M. J., & Jahn, D. (2004) · Springer eBooks · doi:10.1007/978-1-4419-9088-4_4 · Cited by 2 · see citing works
Flumeri, C. D., Acheson, N. H., & Keng, T. (1997) · Canadian Journal of Microbiology · doi:10.1139/m97-114 · Cited by 2 · see citing works
Cloning, Sequencing and Regulation of the Rhodobacter Capsulatus hemH Gene.
Kanazireva, E. (1997) · doi:10.31390/gradschool_disstheses.6494 · Cited by 2 · see citing works
The liver in uroporphyria : a biochemical and morphological study
Siersema, P. D. (1993) · RePub (Erasmus University Rotterdam)
Heme biosynthesis: structure-activity studies of murine ferrochelatase
Shi, Z. (2006) · Digital Commons - University of South Florida (University of South Florida)
FERROCHELATASE AND RELATED ENZYMES
Barton, L. L. (1993) · Elsevier eBooks · doi:10.1016/b978-0-12-079870-4.50018-1
Heme is an essential prosthetic group and a key regulator during erythropoiesis
Tong, J., Shi, L., Liu, J., Jie, G. A. O., & YaPu, L. I. (2017) · Scientia Sinica Vitae · doi:10.1360/n052017-00274
Moore, M. R., McColl, K. E. L., Rimington, C., & Goldberg, A. (1987) · Springer eBooks · doi:10.1007/978-1-4684-1277-2_13
Uroporphyrinogen decarboxylase
Schomburg, D., & Salzmann, M. (1990) · Springer eBooks · doi:10.1007/978-3-642-86605-0_33
Milestones in the Elucidation of Heme Biosynthesis
Dailey, H. A. (2026) · Chemical Reviews · doi:10.1021/acs.chemrev.6c00368
Lelli, S. M. (2013) · Repositorio Digital Institucional de la Universidad de Buenos Aires (Universidad de Buenos Aires)
- Authors
- Straka, J. G., & Kushner, J. P.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1983-09-27
- Volume
- 22
- Issue
- 20
- Pages
- 4664-4672
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W1991671513
- Cited by
- 76
- References
- 24
- Citations by year
- 2026: 1 · 2017: 1 · 2016: 1 · 2013: 1
- FWCI
- 1.54
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (2s07rr005428-30, 2t32am007115-11)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Porphyrin Metabolism and Disorders
- Physical Sciences › Materials Science › Materials Chemistry › Porphyrin and Phthalocyanine Chemistry
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Neonatal Health and Biochemistry
- Keywords
- uroporphyrinogen decarboxylase, enzyme purification, bovine liver, uroporphyrinogen III, porphyrin synthesis, enzyme kinetics, active site, sulfhydryl groups, metal ion inhibition
- MeSH terms
- Animals, Carboxy-Lyases, Cations, Divalent, Cattle, Kinetics, Liver, Molecular Weight, Osmolar Concentration, Salts, Substrate Specificity, Uroporphyrinogen Decarboxylase
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review