Cited by 103Lipid Membrane Structure and BehaviorLipid metabolism and biosynthesisBacterial Genetics and Biotechnology

Metabolic changes of membrane lipid composition in Acholeplasma laidlawii by hydrocarbons, alcohols, and detergents: arguments for effects on lipid packing

Wieslander, Aake, Rilfors, Leif, Lindblom, Goeran·1986·Biochemistry

Abstract

The packing of lipids into different aggregates, such as spheres, rods, or bilayers, is dependent on the hydrophobic volume, the hydrocarbon-water interfacial area, and the hydrocarbon chain length of the participating molecules, according to the self-assembly theory [Israelachvili, J. N., Marcelja, S., & Horn, R. G. (1980) Q. Rev. Biophys. 13, 121-200]. The origin of the participating molecules should be of no importance with respect to their abilities to affect the above-mentioned parameters. In this investigation, Acholeplasma laidlawii, with a defined acyl chain composition of the membrane lipids, has been grown in the presence of three different classes of foreign molecules, known to partition into model and biological membranes. This results in an extensive metabolic alteration in the lipid polar head group composition, which is expressed as changes in the molar ratio between the lipids monoglucosyldiglyceride (MGDG) and diglucosyldiglyceride (DGDG), forming reversed hexagonal and lamellar phases in excess water, respectively. The formation of nonlamellar phases by A. laidlawii lipids depends critically upon the MGDG concentration [Lindblom, G., Brentel, I., Sjölund, M., Wikander, G., & Wieslander, A. (1986) Biochemistry (preceding paper in this issue)]. The foreign molecules tested belong to the following groups: nonpolar organic solvents, alcohols, and detergents. Their effects on the gel to liquid crystalline phase transition temperature (Tm), on the order parameter of the acyl chains, and on the phase equilibria between lamellar and nonlamellar liquid crystalline phases in lipid-water model systems are known in several instances.(ABSTRACT TRUNCATED AT 250 WORDS)

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Crossref · 10.1021/bi00371a038

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Authors
Wieslander, A., Rilfors, L., & Lindblom, G.
Venue
Biochemistry
Published
1986-11-18
Volume
25
Issue
23
Pages
7511-7517
ISSN
  • 0006-2960 (ISSN-L)
  • 1520-4995
OpenAlex
W2037154092
Cited by
103
References
49
Citations by year
2024: 1 · 2023: 1 · 2021: 1 · 2020: 1 · 2019: 4 · 2016: 3 · 2015: 1 · 2013: 5 · 2012: 1
FWCI
3.16
Type
journal article
Language
en
Open access
closed
Locations
  • Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Acholeplasma laidlawii, membrane lipid composition, lipid packing, hydrocarbons, alcohol chemistry, detergents, nonpolar organic solvents, lipid phase transition, gel to liquid-crystalline phase transition, lipid metabolism
MeSH terms
Acholeplasma laidlawii, Alcohols, Cell Membrane, Detergents, Diglycerides, Lipid Bilayers, Membrane Lipids, Phospholipids, Solvents, Structure-Activity Relationship, Surface-Active Agents

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