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The Mr 93,000 polypeptide of the postsynaptic glycine receptor complex is a peripheral membrane protein
Abstract
The glycine receptor of mammalian spinal cord is an oligomeric membrane protein that, after affinity purification on aminostrychnine-agarose or immobilized antibody, contains three polypeptides of Mr 48,000, 58,000, and 93,000. Here, the association and the properties of the polypeptides of the rat glycine receptor were investigated. Upon phase partitioning in the nonionic detergent Triton X-114, the three receptor polypeptides behaved as a hydrophilic protein complex exhibiting phospholipid binding. Sucrose gradient centrifugation or gel filtration in the presence of dithiothreitol and Triton X-100 separated the Mr 93,000 polypeptide from the Mr 48,000 and 58,000 polypeptides, which harbor the antagonist binding site of the glycine receptor. Alkaline or dimethylmaleic acid anhydride treatment of crude synaptic membrane fractions resulted in extraction of the Mr 93,000 polypeptide. Lectin binding was observed for the Mr 48,000 and 58,000 glycine receptor subunits but not the Mr 93,000 polypeptide. These results indicate that the Mr 93,000 polypeptide is a peripheral membrane protein that is located at the cytoplasmic face of the postsynaptic glycine receptor complex.
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Cited by
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Jentsch, T. J., Stein, V., Weinreich, F., & Zdebik, A. A. (2002) · Physiological Reviews · doi:10.1152/physrev.00029.2001 · Cited by 1,256
Clustered dynamics of inhibitory synapses and dendritic spines in the adult neocortex.
Chen, J. L., Villa, K. L., Cha, J. W., So, P. T. C., Kubota, Y., & Nedivi, E. (2012) · Neuron · doi:10.1016/j.neuron.2012.02.030 · Cited by 341
The 93-kDa glycine receptor-associated protein binds to tubulin.
Kirsch, J., Langosch, D., Prior, P., Littauer, U. Z., Schmitt, B., & Betz, H. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)54560-9 · Cited by 273
Self-citationSia, G.-M., Béïque, J.-C., Rumbaugh, G., Cho, R., Worley, P. F., & Huganir, R. L. (2007) · Neuron · doi:10.1016/j.neuron.2007.06.020 · Cited by 201
Wheal, H. V., Chen, Y., Mitchell, J., Schachner, M., Maerz, W., Wieland, H., van Rossum, D., & Kirsch, J. (1998) · Progress in Neurobiology · doi:10.1016/s0301-0082(98)00026-4 · Cited by 93
Metadata
- Authors
- Schmitt, B., Knaus, P., Becker, C. M., & Betz, H.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1987-02-01
- Volume
- 26
- Issue
- 3
- Pages
- 805-811
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2049092897
- Cited by
- 186
- References
- 0
- FWCI
- 4.39
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: The Mr 93,000 polypeptide of the postsynaptic glycine receptor complex is a peripheral membrane protein. https://app.cochat.ai/papers/10.1021/bi00377a022
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- Schmitt, B., Knaus, P., Becker, C. M., & Betz, H. (1987). The Mr 93,000 polypeptide of the postsynaptic glycine receptor complex is a peripheral membrane protein. Biochemistry. https://doi.org/10.1021/bi00377a022
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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