Clustered dynamics of inhibitory synapses and dendritic spines in the adult neocortex.
Open accessAbstract
A key feature of the mammalian brain is its capacity to adapt in response to experience, in part by remodeling of synaptic connections between neurons. Excitatory synapse rearrangements have been monitored in vivo by observation of dendritic spine dynamics, but lack of a vital marker for inhibitory synapses has precluded their observation. Here, we simultaneously monitor in vivo inhibitory synapse and dendritic spine dynamics across the entire dendritic arbor of pyramidal neurons in the adult mammalian cortex using large-volume, high-resolution dual-color two-photon microscopy. We find that inhibitory synapses on dendritic shafts and spines differ in their distribution across the arbor and in their remodeling kinetics during normal and altered sensory experience. Further, we find inhibitory synapse and dendritic spine remodeling to be spatially clustered and that clustering is influenced by sensory input. Our findings provide in vivo evidence for local coordination of inhibitory and excitatory synaptic rearrangements.
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Monocular deprivation significantly increases coordinated dynamics of spines and inhibitory synapses nearby in layer 2/3 pyramidal neurons.
“Monocular deprivation significantly increases coordinated dynamics of spines and the inhibitory synapses nearby in layer 2/3 pyramidal neurons (Chen et al., 2012).”
From Spatiotemporal dynamics of dendritic spines in the living brain (2014) · cites this paper as Chen et al., 2012
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- Authors
- Chen, J. L., Villa, K. L., Cha, J. W., So, P. T. C., Kubota, Y., & Nedivi, E.
- Affiliations
- Massachusetts Institute of Technology (US)
- The Graduate University for Advanced Studies, SOKENDAI (JP)
- Corresponding author
- Nedivi, Elly
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2012-04
- Volume
- 74
- Issue
- 2
- Pages
- 361-373
- ISSN
- 0896-6273 (ISSN-L)
- PMC
- PMC3340582
- OpenAlex
- W2056431599
- Cited by
- 341
- References
- 52
- Citations by year
- 2026: 12 · 2025: 19 · 2024: 17 · 2023: 18 · 2022: 21 · 2021: 22 · 2020: 23 · 2019: 27 · 2018: 32 · 2017: 33 · 2016: 29 · 2015: 22 · 2014: 34 · 2013: 22 · 2012: 9
- FWCI
- 11.46
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by-nc)
- Elsevier eBooks
- Funders
- Massachusetts Institute of Technology
- Japan Society for the Promotion of Science
- National Eye Institute (R01EY017656, R01EY011894)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- inhibitory synapses, dendritic spines, synaptic plasticity, synaptic remodeling, pyramidal neurons, dendritic branching, sensory experience, in vivo imaging
- MeSH terms
- Animals, Carrier Proteins, Embryo, Mammalian, Functional Laterality, Luminescent Proteins, Membrane Proteins, Mice, Inbred C57BL, Models, Biological, Neural Inhibition, Neurons, Photic Stimulation, Sensory Deprivation, Synapses, Microscopy, Immunoelectron, Nonlinear Dynamics, Electroporation, Neocortex, Imaging, Three-Dimensional, Dendritic Spines, Mice, Optics and Photonics
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