The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Amino acids of the Torpedo marmorata acetylcholine receptor .alpha. subunit labeled by a photoaffinity ligand for the acetylcholine binding site
Abstract
The acetylcholine-binding sites on the native, membrane-bound acetylcholine receptor from Torpedo marmorata were covalently labeled with the photoaffinity reagent [3H]-p-(dimethylamino)-benzenediazonium fluoroborate (DDF) in the presence of phencyclidine by employing an energy-transfer photolysis procedure. The alpha-chains isolated from receptor-rich membranes photolabeled in the absence or presence of carbamoylcholine were cleaved with CNBr and the radiolabeled fragments purified by high-performance liquid chromatography. Amino acid and/or sequence analysis demonstrated that the alpha-chain residues Trp-149, Tyr-190, Cys-192, and Cys-193 and an unidentified residue(s) in the segment alpha 31-105 were all labeled by the photoaffinity reagent in an agonist-protectable manner. The labeled amino acids are located within three distinct regions of the large amino-terminal hydrophilic domain of the alpha-subunit primary structure and plausibly lie in proximity to one another at the level of the acetylcholine-binding sites in the native receptor. These findings are in accord with models proposed for the transmembrane topology of the alpha-chain that assign the amino-terminal segment alpha 1-210 to the synaptic cleft. Furthermore, the results suggest that the four identified [3H]DDF-labeled residues, which are conserved in muscle and neuronal alpha-chains but not in the other subunits, may be directly involved in agonist binding.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Structural homology of Torpedo californica acetylcholine receptor subunits
Noda, M., Takahashi, H., Tanabe, T., Toyosato, M., Kikyotani, S., Furutani, Y., Hirose, T., Takashima, H., Inayama, S., Miyata, T., et al. (1983) · Nature · doi:10.1038/302528a0 · Cited by 799
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Acetylcholine Receptor: An Allosteric Protein
Changeux, J.-P., Devillers-Thiéry, A., & Chemouilli, P. (1984) · Science · doi:10.1126/science.6382611 · Cited by 623
Self-citationIsolation of a cDNA clone coding for a possible neural nicotinic acetylcholine receptor α-subunit
Boulter, J., Evans, K., Goldman, D., Martin, G., Treco, D., Heinemann, S., & Patrick, J. (1986) · Nature · doi:10.1038/319368a0 · Cited by 437
Kao, P. N., Dwork, A. J., Kaldany, R. R., Silver, M. L., Wideman, J., Stein, S., & Karlin, A. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)71257-3 · Cited by 410
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Nicotinic receptor of acetylcholine: structure of an oligomeric integral membrane protein
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Self-citationExpression of functional acetylcholine receptor from cloned cDNAs
Mishina, M., Kurosaki, T., Tobimatsu, T., Morimoto, Y., Noda, M., Yamamoto, T., Terao, M., Lindstrom, J., Takahashi, T., Kuno, M., et al. (1984) · Nature · doi:10.1038/307604a0 · Cited by 369
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Cited by
Le Novere, N., Zoli, M., & Changeux, J. (1996) · European Journal of Neuroscience · doi:10.1111/j.1460-9568.1996.tb01206.x · Cited by 385
Self-citationBrain nicotinic receptors: structure and regulation, role in learning and reinforcement
Changeux, J.-P., Bertrand, D., Corringer, P.-J., Dehaene, S., Edelstein, S., Léna, C., Le Novère, N., Marubio, L., Picciotto, M., & Zoli, M. (1998) · Brain Research Reviews · doi:10.1016/s0165-0173(97)00040-4 · Cited by 322
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Metadata
- Authors
- Dennis, M., Giraudat, J., Kotzyba-Hibert, F., Goeldner, M., Hirth, C., Chang, J. Y., Lazure, C., Chretien, M., & Changeux, J. P.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1988-04-05
- Volume
- 27
- Issue
- 7
- Pages
- 2346-2357
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2010244456
- Cited by
- 317
- References
- 38
- FWCI
- 9.40
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Amino acids of the Torpedo marmorata acetylcholine receptor .alpha. subunit labeled by a photoaffinity ligand for the acetylcholine binding site. https://app.cochat.ai/papers/10.1021/bi00407a016
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- Dennis, M., Giraudat, J., Kotzyba-Hibert, F., Goeldner, M., Hirth, C., Chang, J. Y., Lazure, C., Chretien, M., & Changeux, J. P. (1988). Amino acids of the Torpedo marmorata acetylcholine receptor .alpha. subunit labeled by a photoaffinity ligand for the acetylcholine binding site. Biochemistry. https://doi.org/10.1021/bi00407a016
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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