The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Heteronuclear three-dimensional NMR spectroscopy of the inflammatory protein C5a
Abstract
The utility of three-dimensional heteronuclear NMR spectroscopy for the assignment of 1H and 15N resonances of the inflammatory protein C5a (MW 8500), uniformly labeled with 15N, is demonstrated at a protein concentration of 0.7 mM. It is shown that dramatic simplification of the 2D nuclear Overhauser effect spectrum (NOESY) is obtained by editing with respect to the frequency of the 15N heteronucleus in a third dimension. The improved resolution in the 3D experiment largely facilitates the assignment of protein NMR spectra and allows for the determination of distance constraints from otherwise overlapping NOE cross peaks for purposes of 3D structure determination. The results show that 15N heteronuclear 3D NMR can facilitate the structure determination of small proteins and promises to be a useful tool for the study of larger systems that cannot be studied by conventional 2D NMR techniques.
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Citations
Cites
Correlation of proton and nitrogen-15 chemical shifts by multiple quantum NMR
Bax, A., Griffey, R. H., & Hawkins, B. L. (1983) · Journal of Magnetic Resonance (1969) · doi:10.1016/0022-2364(83)90241-x · Cited by 1,259
An improved sequence for broadband decoupling: WALTZ-16
Shaka, A. J., Keeler, J., Frenkiel, T., & Freeman, R. (1983) · Journal of Magnetic Resonance (1969) · doi:10.1016/0022-2364(83)90207-x · Cited by 1,039
Fesik, S. W., & Zuiderweg, E. R. P. (1988) · Journal of Magnetic Resonance (1969) · doi:10.1016/0022-2364(88)90144-8 · Cited by 379
Self-citationThree-dimensional NMR spectroscopy of a protein in solution
Oschkinat, H., Griesinger, C., Kraulis, P. J., et al. (1988) · Nature · doi:10.1038/332374a0 · Cited by 248
Zuiderweg, E. R. P., Nettesheim, D. G., Mollison, K. W., & Carter, G. W. (1989) · Biochemistry · doi:10.1021/bi00427a025 · Cited by 100
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Cited by
Pectins: structure, biosynthesis, and oligogalacturonide-related signaling
Ridley, B. L., O'Neill, M. A., & Mohnen, D. (2001) · Phytochemistry · doi:10.1016/s0031-9422(01)00113-3 · Cited by 1,912
NMR Spectroscopy in the structural elucidation of oligosaccharides and glycosides
Agrawal, P. K. (1992) · Phytochemistry · doi:10.1016/0031-9422(92)83678-r · Cited by 1,454
Feng, S., Chen, J. K., Yu, H., Simon, J. A., & Schreiber, S. L. (1994) · Science · doi:10.1126/science.7526465 · Cited by 687
Fischer, D., Mukrasch, M. D., Biernat, J., Bibow, S., Blackledge, M., Griesinger, C., Mandelkow, E., & Zweckstetter, M. (2009) · Biochemistry · doi:10.1021/bi901090m · Cited by 149
Brath, U., & Akke, M. (2009) · Journal of Molecular Biology · doi:10.1016/j.jmb.2009.01.047 · Cited by 36
Metadata
- Authors
- Zuiderweg, E. R. P., & Fesik, S. W.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1989-03-01
- Volume
- 28
- Issue
- 6
- Pages
- 2387-2391
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2003458079
- Cited by
- 329
- References
- 5
- FWCI
- 18.91
- Type
- journal article
- Language
- en
- Open access
- closed
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