Superoxide Reduction Mechanism of Archaeoglobus fulgidus One-Iron Superoxide Reductase
Abstract
Superoxide reductases (SORs), iron-centered enzymes responsible for reducing superoxide (O2(-)) to hydrogen peroxide, are found in many anaerobic and microaerophilic prokaryotes. The rapid reaction with an exogenous electron donor renders the reductase activity catalytic. Here, we demonstrate using pulse radiolysis that the initial reaction between O2(-) and Archaeoglobus fulgidus neelaredoxin, a one-iron SOR, leads to a short-lived transient that immediately disappears to yield a solvent-bound ferric species in acid-base equilibrium. Through comparison of wild-type neelaredoxin with mutants lacking the ferric ion coordinating glutamate, we demonstrate that the remaining step is related to the final coordination of this ligand to the oxidized metal center and kinetically characterize it for the first time, by pulse radiolysis and stopped-flow kinetics. The way exogenous phosphate perturbs the kinetics of superoxide reduction by neelaredoxin and mutant proteins was also investigated.
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UV-visible spectroscopy has been applied to study the catalytic activity of SOR.
“UV-visible spectroscopy has been applied to study the catalytic activity of SOR [92,93,94,95,96,97,98,99].”
From Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity (2023) · cites this paper as [92]
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Superoxide dismutases and superoxide reductases
Sheng, Y., Abreu, I. A., Cabelli, D. E., Maroney, M. J., Miller, A.-F., Teixeira, M., & Valentine, J. S. (2014) · Chemical Reviews · doi:10.1021/cr4005296 · Cited by 1,081 · see citing works
Self-citationSuperoxide Anion Chemistry—Its Role at the Core of the Innate Immunity
Andrés, C. M. C., Pérez de la Lastra, J. M., Andrés Juan, C., Plou, F. J., & Pérez-Lebeña, E. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24031841 · Cited by 342 · see citing works
Reductive elimination of superoxide: Structure and mechanism of superoxide reductases
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Self-citationSuperoxide reduction by Archaeoglobus fulgidus desulfoferrodoxin: Comparison with neelaredoxin
Rodrigues, J. V., Saraiva, L. M., Abreu, I. A., Teixeira, M., & Cabelli, D. E. (2007) · JBIC Journal of Biological Inorganic Chemistry · doi:10.1007/s00775-006-0182-x · Cited by 40 · see citing works
Self-citationRodrigues, J. V., Victor, B. L., Huber, H., Saraiva, L. M., Soares, C. M., Cabelli, D. E., & Teixeira, M. (2008) · JBIC Journal of Biological Inorganic Chemistry · doi:10.1007/s00775-007-0313-z · Cited by 24 · see citing works
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- Authors
- Rodrigues, J. V., Abreu, I. A., Cabelli, D., & Teixeira, M.
- Affiliations
- Brookhaven National Laboratory (US)
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2006-07-08
- Volume
- 45
- Issue
- 30
- Pages
- 9266-9278
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2088161991
- Cited by
- 46
- References
- 9
- Citations by year
- 2024: 1 · 2023: 1 · 2021: 1 · 2020: 1 · 2019: 3 · 2018: 1 · 2017: 1 · 2016: 2 · 2015: 2 · 2014: 3 · 2013: 1 · 2012: 4
- FWCI
- 3.54
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Energy
- Brookhaven National Laboratory
- Topics
- Physical Sciences › Chemistry › Inorganic Chemistry › Metal-Catalyzed Oxygenation Mechanisms
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Metalloenzymes and iron-sulfur proteins
- Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
- Keywords
- superoxide reductase, Archaeoglobus fulgidus, acid-base balance, stopped-flow kinetics, pulse radiolysis, hydrogen peroxide production
- MeSH terms
- Bacterial Proteins, Hydrogen Peroxide, Hydrogen-Ion Concentration, Kinetics, Oxidation-Reduction, Oxidoreductases, Superoxide Dismutase, Archaeoglobus fulgidus, Archaeal Proteins, Iron-Binding Proteins
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