CoChat
Sign Up
Cited by 30

The DOI matches its Crossref record.

Guanine Nucleotide Binding Properties of Rac2 Mutant Proteins and Analysis of the Responsiveness to Guanine Nucleotide Dissociation Stimulator,

Xu, Xuemin, Wang, Yan, Barry, David C., Chanock, Stephen J., Bokoch, Gary M.·1997·Biochemistry
DOI 10.1021/bi962059h
Open this paper in CoChat — free account

Abstract

The Rac GTPases are currently being subjected to intensive study due to their involvement in a wide array of cellular phenomena. Many studies of Rac function have relied upon the use of relatively uncharacterized Rac dominant active, dominant negative, and effector domain mutants on the basis of the analogy to Ras structure. We have generated and purified such Rac2 mutants and characterized their guanine nucleotide binding properties in vitro. The Rac2 G12V and Q61L activating mutations were shown to hydrolyze bound GTP very slowly and were unresponsive to p190 Rac GTPase-activating protein. Distinct differences in the kinetics of nucleotide binding to individual mutant proteins were observed, accounting for the behavior of these proteins in biological assays. The structural features required for the responsiveness of Rac2 to the guanine nucleotide exchange protein smgGDS were examined. We show that guanine nucleotide exchange by smgGDS is dependent upon intact switch 1 and switch 2 regions in Rac2. Functional interactions between the switch 1 and switch 2 regions and the G12V mutation of Rac2 are described. These data form the basis for rational use of Rac mutants in biological studies.

Overview

Integrity signals

Registry

Crossref · 10.1021/bi962059h

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 47

  1. Abo A., 1992, J. Biol. Chem., 267
  2. Bagrodia S., 1995, J. Biol. Chem., 270
  3. Barbacid M., 1987, Annu. Rev. Biochem., 56, 10.1146/annurev.bi.56.070187.004023 · DOI · Paper page
  4. Boguski M. S., 1993, Nature 366, 643−654
  5. Bokoch G. M., 1995, Trends Cell Biol. 5, 109−113
  6. Bromberg Y., 1994, J. Biol. Chem., 269, 10.1016/S0021-9258(17)37243-5 · DOI
  7. Brondyk W. H., 1993, J. Biol. Chem., 268, 10.1016/S0021-9258(18)98366-3 · DOI
  8. Chen S. Y., 1994, Oncogene 9, 2691−2698
  9. Chuang T. H., 1994, Biochem. J., 303, 10.1042/bj3030761 · DOI
  10. Chuang T. H., 1995, Proc. Natl. Acad. Sci. U.S.A. 92, 10282−10286
  11. Der C. J., 1989, C., & Liu, 119
  12. Diekmann D., 1995, EMBO J., 14, 10.1002/j.1460-2075.1995.tb00214.x · DOI · Paper page
  13. Dorseuil O., 1996, J. Biol. Chem., 271, 88, 10.1074/jbc.271.1.83 · DOI
  14. Feig L. A., 1988, Mol. Cell. Biol. 8, 3235−3243
  15. Grand R. J. A., 1991, Biochem. J., 279
  16. Habets G. G. M., 1994, Cell 77, 537−549
  17. Heyworth P. G., 1993, Mol. Biol. Cell 4, 261−269
  18. Hiraoka K., 1992, Biochem. Biophys. Res. Commun. 182, 921−930
  19. Hwang Y. W., 1993, J. Biol. Chem., 268
  20. Jung V., 1994, Mol. Cell. Biol. 14, 3707−3718
  21. Kaibuchi K., 1991, Mol. Cell. Biol. 11, 2873−2880
  22. Knaus U. G., 1991, Science 254, 1512−1515
  23. Knaus U. G., 1992, J. Biol. Chem., 267
  24. Knaus U. G., 1995, Science 269, 221−223
  25. Krengel U., 1990, Cell 62, 539−548
  26. Manser E., 1994, Nature, 367, 46, 10.1038/367040a0 · DOI
  27. Marshall M. S., 1993, Trends Biol. Sci. 18, 250−254
  28. Menard L., 1992, Eur. J. Biochem., 206, 10.1111/j.1432-1033.1992.tb16957.x · DOI
  29. Michiels F., 1995, Nature 375, 338−340
  30. Mirisola M. G., 1994, J. Biol. Chem., 269, 10.1016/S0021-9258(17)40743-5 · DOI
  31. Mistou M. Y., 1992, EMBO J., 11, 10.1002/j.1460-2075.1992.tb05303.x · DOI
  32. Mosteller R. D., 1994, Mol. Cell. Biol. 14, 1104−1112
  33. Pai E. F., 1990, EMBO J., 9
  34. Qiu R. G., 1995, Nature 374, 457−459
  35. Quilliam L. A., 1994, Mol. Cell. Biol. 14, 1113−1121
  36. Quinn M. T., 1993, J. Biol. Chem., 268
  37. Ridley A. J., 1994, BioEssays 16, 321−327
  38. Ridley A. J., 1992, Cell 70, 401−410
  39. Segal M., 1993, Proc. Natl. Acad. Sci. U.S.A. 90, 5564−5568
  40. Tong L., 1989, Nature, 337, 93, 10.1038/337090a0 · DOI
  41. Verrotti A. C., 1992, EMBO J., 11, 10.1002/j.1460-2075.1992.tb05353.x · DOI
  42. Voncken J. W., 1995, Cell 80, 719−728
  43. Wray W., 1981, Anal. Biochem. 118, 197−203
  44. Xu X., 1994, J. Biol. Chem., 269
  45. Yamamoto T., 1990, J. Biol. Chem., 265
  46. Zhang S., 1995, J. Biol. Chem., 270
  47. Abbreviations PγS, guanosine 5‘-triphosphate

Cited by

See all 30

Metadata

Authors
Xu, X., Wang, Y., Barry, D. C., Chanock, S. J., & Bokoch, G. M.
Venue
Biochemistry
Publisher
American Chemical Society (ACS)
Published
1997-01-01
Volume
36
Issue
3
Pages
626-632
ISSN
  • 0006-2960
  • 1520-4995
DOI
10.1021/bi962059h
Cited by
30
References
0
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Guanine Nucleotide Binding Properties of Rac2 Mutant Proteins and Analysis of the Responsiveness to Guanine Nucleotide Dissociation Stimulator,. https://app.cochat.ai/papers/10.1021/bi962059h
The paper
Xu, X., Wang, Y., Barry, D. C., Chanock, S. J., & Bokoch, G. M. (1997). Guanine Nucleotide Binding Properties of Rac2 Mutant Proteins and Analysis of the Responsiveness to Guanine Nucleotide Dissociation Stimulator,. Biochemistry. https://doi.org/10.1021/bi962059h