Polydopamine-Assisted Osteoinductive Peptide Immobilization of Polymer Scaffolds for Enhanced Bone Regeneration by Human Adipose-Derived Stem Cells
Abstract
Immobilization of osteoinductive molecules, including growth factors or peptides, on polymer scaffolds is critical for improving stem cell-mediated bone tissue engineering. Such molecules provide osteogenesis-stimulating signals for stem cells. Typical methods used for polymeric scaffold modification (e.g., chemical conjugation or physical adsorption), however, have limitations (e.g., multistep, complicated procedures, material denaturation, batch-to-batch inconsistency, and inadequate conjugation) that diminish the overall efficiency of the process. Therefore, in this study, we report a biologically inspired strategy to prepare functional polymer scaffolds that efficiently regulate the osteogenic differentiation of human adipose-derived stem cells (hADSCs). Polymerization of dopamine (DA), a repeated motif observed in mussel adhesive protein, under alkaline pH conditions, allows for coating of a polydopamine (pDA) layer onto polymer scaffolds. Our study demonstrates that predeposition of a pDA layer facilitates highly efficient, simple immobilization of peptides derived from osteogenic growth factor (bone morphogenetic protein-2; BMP-2) on poly(lactic-co-glycolic acid) (PLGA) scaffolds via catechol chemistry. The BMP-2 peptide-immobilized PLGA scaffolds greatly enhanced in vitro osteogenic differentiation and calcium mineralization of hADSCs using either osteogenic medium or nonosteogenic medium. Furthermore, transplantation of hADSCs using pDA-BMP-2-PLGA scaffolds significa…
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Upon immobilization of BMP, the property of osteoblast cells responsive to polymeric materials can be remarkably improved.
“Upon immobilization of BMP, the property of osteoblast cells responsive to polymeric materials can be remarkably improved”
From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [93]
Material mineralization can be expedited in the presence of immobilized growth factors.
“and the material mineralization can be expedited in the presence of immobilized growth factors [91–95].”
From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [93]
Cites
Mussel‐Inspired Polydopamine Coating as a Universal Route to Hydroxyapatite Crystallization
Ryu, J., Ku, S. H., Lee, H., & Park, C. B. (2010) · Advanced Functional Materials · doi:10.1002/adfm.200902347 · Cited by 778 · see citing works
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Ren, X., Feng, Y., Guo, J., Wang, H., Li, Q., Yang, J., Hao, X., Lv, J., Ma, N., & Li, W. (2015) · Chemical Society Reviews · doi:10.1039/c4cs00483c · Cited by 573 · see citing works
Chuah, Y. J., Koh, Y. T., Lim, K., Menon, N. V., Wu, Y., & Kang, Y. (2015) · Scientific Reports · doi:10.1038/srep18162 · Cited by 262 · see citing works
Polydopamine-Assisted Surface Modification for Bone Biosubstitutes
Huang, S., Liang, N., Hu, Y., Zhou, X., & Abidi, N. (2016) · BioMed Research International · doi:10.1155/2016/2389895 · Cited by 97 · see citing works
“[92] E.”
Background
- Authors
- Ko, E., Yang, K., Shin, J., & Cho, S.-W.
- Affiliations
- Yonsei University (KR)
- Corresponding author
- Cho, Seung-Woo
- Venue
- Biomacromolecules
- Publisher
- American Chemical Society (ACS)
- Published
- 2013-08-29
- Volume
- 14
- Issue
- 9
- Pages
- 3202-3213
- ISSN
- 1525-7797 (ISSN-L)
- 1526-4602
- OpenAlex
- W1977214686
- Cited by
- 226
- References
- 1
- Citations by year
- 2026: 8 · 2025: 10 · 2024: 13 · 2023: 11 · 2022: 17 · 2021: 15 · 2020: 18 · 2019: 18 · 2018: 26 · 2017: 27 · 2016: 26 · 2015: 20 · 2014: 13 · 2013: 3
- FWCI
- 11.39
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biomacromolecules (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Health Sciences › Medicine › Urology › Periodontal Regeneration and Treatments
- Keywords
- polydopamine, peptide immobilization, BMP-2, human adipose-derived stem cells, PLGA scaffolds, osteogenic differentiation, bone regeneration, catechol chemistry, mussel adhesive proteins, scaffold surface modification, critical-size calvarial defect, calcium mineralization
- MeSH terms
- Polylactic Acid-Polyglycolic Acid Copolymer, Adipose Tissue, Amino Acid Sequence, Animals, Bone Regeneration, Cell Differentiation, Cells, Cultured, Collagen, Female, Humans, Indoles, Mice, Inbred BALB C, Mice, Nude, Molecular Sequence Data, Osteogenesis, Peptide Fragments, Polyglycolic Acid, Polymers, Radiography, Skull, Surface Properties, Lactic Acid, Coated Materials, Biocompatible, Regenerative Medicine, Mice, Osteopontin, Adult Stem Cells, Bone Morphogenetic Protein 2, Immobilized Proteins
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