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Protein Separations Using Colloidal Magnetic Nanoparticles
Abstract
Phospholipid‐coated colloidal magnetic nanoparticles with mean magnetite core size of 8 nm are shown to be effective ion exchange media for the recovery and separation of proteins from protein mixtures. These particles have high adsorptive capacities (up to 1200 mg protein/mL adsorbent, an order of magnitude larger than the best commercially available adsorbents) and exhibit none of the diffusional resistances offered by conventional porous ion exchange media. Protein‐laden particles are readily recovered from the feed solution using high‐gradient magnetic filtration.
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Citations
Cited by
Laurent, S., Forge, D., Port, M., Roch, A., Robic, C., Vander Elst, L., & Muller, R. N. (2008) · Chemical Reviews · doi:10.1021/cr068445e · Cited by 6,852
Low-Field Magnetic Separation of Monodisperse Fe 3 O 4 Nanocrystals
Yavuz, C. T., Mayo, J. T., Yu, W. W., Prakash, A., Falkner, J. C., Yean, S., Cong, L., Shipley, H. J., Kan, A., Tomson, M., et al. (2006) · Science · doi:10.1126/science.1131475 · Cited by 1,250
Biomedical Nanomagnetics: A Spin Through Possibilities in Imaging, Diagnostics, and Therapy
Krishnan, K. M. (2010) · IEEE Transactions on Magnetics · doi:10.1109/tmag.2010.2046907 · Cited by 819
Afkhami, A., & Moosavi, R. (2010) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2009.09.066 · Cited by 667
Synthesis of monodisperse iron oxide nanocrystals by thermal decomposition of iron carboxylate salts
Yu, W. W., Falkner, J. C., Yavuz, C. T., & Colvin, V. L. (2004) · Chemical Communications · doi:10.1039/b409601k · Cited by 621
Magnetic techniques for the isolation and purification of proteins and peptides
Safarik, I., & Safarikova, M. (2004) · BioMagnetic Research and Technology · doi:10.1186/1477-044x-2-7 · Cited by 503
Bruce, I. J., & Sen, T. (2005) · Langmuir · doi:10.1021/la050553t · Cited by 440
Oh, J. K., & Park, J. M. (2011) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2010.08.005 · Cited by 427
Protein purification using magnetic adsorbent particles
Franzreb, M., Siemann-Herzberg, M., Hobley, T. J., & Thomas, O. R. T. (2006) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-006-0344-3 · Cited by 340
Grass, R. N., Athanassiou, E. K., & Stark, W. J. (2007) · Angewandte Chemie International Edition · doi:10.1002/anie.200700613 · Cited by 330
Glucose oxidase?magnetite nanoparticle bioconjugate for glucose sensing
Rossi, L. M., Quach, A. D., & Rosenzweig, Z. (2004) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-004-2770-3 · Cited by 314
The use of magnetic nanoparticles in sample preparation devices and tools
Keçili, R., Büyüktiryaki, S., Dolak, İ., & Hussain, C. M. (2020) · Handbook of Nanomaterials in Analytical Chemistry · doi:10.1016/b978-0-12-816699-4.00005-0 · Cited by 64
自组装制备Fe3O4@Au复合纳米粒子用于固定化葡萄糖氧化酶
杨, 婉., 王, 显., 黄, 硕., & 单, 志. (2009) · Chinese Science Bulletin · doi:10.1360/csb2009-54-4-430 · Cited by 23
Metadata
- Authors
- Bucak, S., Jones, D. A., Laibinis, P. E., & Hatton, T. A.
- Venue
- Biotechnology Progress
- Publisher
- Wiley
- Published
- 2003-01
- Volume
- 19
- Issue
- 2
- Pages
- 477-484
- ISSN
- 1520-6033 (ISSN-L)
- 8756-7938
- OpenAlex
- W2151470854
- Cited by
- 324
- References
- 0
- FWCI
- 6.97
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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