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Optimization of Ammonia Fiber Expansion (AFEX) Pretreatment and Enzymatic Hydrolysis of Miscanthus x giganteus to Fermentable Sugars
Open accessAbstract
Miscanthus x giganteus is a tall perennial grass whose suitability as an energy crop is presently being appraised. There is very little information on the effect of pretreatment and enzymatic saccharification of Miscanthus to produce fermentable sugars. This paper reports sugar yields during enzymatic hydrolysis from ammonia fiber expansion (AFEX) pretreated Miscanthus. Pretreatment conditions including temperature, moisture, ammonia loading, residence time, and enzyme loadings are varied to maximize hydrolysis yields. In addition, further treatments such as soaking the biomass prior to AFEX as well as washing the pretreated material were also attempted to improve sugar yields. The optimal AFEX conditions determined were 160 degrees C, 2:1 (w/w) ammonia to biomass loading, 233% moisture (dry weight basis), and 5 min reaction time for water-soaked Miscanthus. Approximately 96% glucan and 81% xylan conversions were achieved after 168 h enzymatic hydrolysis at 1% glucan loading using 15 FPU/(g of glucan) of cellulase and 64 p-NPGU/(g of glucan) of beta-glucosidase along with xylanase and tween-80 supplementation. A mass balance for the AFEX pretreatment and enzymatic hydrolysis process is presented.
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Cites
Features of promising technologies for pretreatment of lignocellulosic biomass
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Miscanthus for renewable energy generation: European Union experience and projections for Illinois.
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Miscanthus: a fast‐growing crop for biofuels and chemicals production
Brosse, N., Dufour, A., Meng, X., Sun, Q., & Ragauskas, A. (2012) · Biofuels, Bioproducts and Biorefining · doi:10.1002/bbb.1353 · Cited by 446
Room temperature ionic liquids as emerging solvents for the pretreatment of lignocellulosic biomass
Mora‐Pale, M., Meli, L., Doherty, T. V., Linhardt, R. J., & Dordick, J. S. (2011) · Biotechnology and Bioengineering · doi:10.1002/bit.23108 · Cited by 384
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Mass Balances and Analytical Methods for Biomass Pretreatment Experiments
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Metadata
- Authors
- Murnen, H. K., Balan, V., Chundawat, S. P. S., Bals, B., daCostaSousa, L., & Dale, B. E.
- Venue
- Biotechnology Progress
- Publisher
- Wiley
- Published
- 2007-08-03
- Volume
- 23
- Issue
- 4
- Pages
- 846-850
- ISSN
- 1520-6033 (ISSN-L)
- 8756-7938
- OpenAlex
- W2092940114
- Cited by
- 165
- References
- 2
- FWCI
- 8.92
- Type
- journal article
- Language
- en
- Open access
- green
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