The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Genetically Encoded Short Peptide Tags for Orthogonal Protein Labeling by Sfp and AcpS Phosphopantetheinyl Transferases
Abstract
Short peptide tags S6 and A1, each 12 residues in length, were identified from a phage-displayed peptide library as efficient substrates for site-specific protein labeling catalyzed by Sfp and AcpS phosphopantetheinyl transferases (PPTases), respectively. S6 and A1 tags were selected for useful levels of orthogonality in reactivities with the PPTases: the catalytic efficiency, kcat/Km of Sfp-catalyzed S6 serine phosphopantetheinylation was 442-fold greater than that for AcpS. Conversely, the kcat/Km of AcpS-catalyzed A1 labeling was 30-fold higher than that for Sfp-catalyzed A1 labeling. S6 and A1 peptide tags can be fused to N- or C-termini of proteins for orthogonal labeling of target proteins in cell lysates or on live cell surfaces. The development of the orthogonal S6 and A1 tags represents a significant enhancement of PPTase-catalyzed protein labeling, allowing tandem or iterative covalent attachment of small molecules of diverse structures to the target proteins with high efficiency and specificity.
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Quadri, L. E. N., Weinreb, P. H., Lei, M., Nakano, M. M., Zuber, P., & Walsh, C. T. (1998) · Biochemistry · doi:10.1021/bi9719861 · Cited by 693
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Sletten, E. M., & Bertozzi, C. R. (2009) · Angewandte Chemie International Edition · doi:10.1002/anie.200900942 · Cited by 3,165
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Fernández-Suárez, M., & Ting, A. Y. (2008) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2531 · Cited by 1,306
Design of a hyperstable 60-subunit protein icosahedron
Hsia, Y., Bale, J. B., Gonen, S., Shi, D., Sheffler, W., Fong, K. K., Nattermann, U., Xu, C., Huang, P.-S., Ravichandran, R., et al. (2016) · Nature · doi:10.1038/nature18010 · Cited by 445
Srikun, D., Albers, A. E., Nam, C. I., Iavarone, A. T., & Chang, C. J. (2010) · Journal of the American Chemical Society · doi:10.1021/ja100117u · Cited by 310
Wasserman, M. R., Pulk, A., Zhou, Z., Altman, R. B., Zinder, J. C., Green, K. D., Garneau-Tsodikova, S., Doudna Cate, J. H., & Blanchard, S. C. (2015) · Nature Communications · doi:10.1038/ncomms8896 · Cited by 77
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Metadata
- Authors
- Zhou, Z., Cironi, P., Lin, A. J., Xu, Y., Hrvatin, S., Golan, D. E., Silver, P. A., Walsh, C. T., & Yin, J.
- Venue
- ACS Chemical Biology
- Publisher
- American Chemical Society (ACS)
- Published
- 2007-04-27
- Volume
- 2
- Issue
- 5
- Pages
- 337-346
- ISSN
- 1554-8929 (ISSN-L)
- 1554-8937
- OpenAlex
- W2328394317
- Cited by
- 230
- References
- 1
- FWCI
- 9.83
- Type
- journal article
- Language
- en
- Open access
- closed
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