The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
Phosphoproteomics for the Masses
Open accessAbstract
Protein phosphorylation serves as a primary mechanism of signal transduction in the cells of biological organisms. Technical advancements over the last several years in mass spectrometry now allow for the large-scale identification and quantitation of in vivo phosphorylation at unprecedented levels. These developments have occurred in the areas of sample preparation, instrumentation, quantitative methodology, and informatics so that today, 10 000-20 000 phosphorylation sites can be identified and quantified within a few weeks. With the rapid development and widespread availability of such data, its translation into biological insight and knowledge is a current obstacle. Here we present an overview of how this technology came to be and is currently applied, as well as future challenges for the field.
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Cites
Ong, S.-E., Blagoev, B., Kratchmarova, I., Kristensen, D. B., Steen, H., Pandey, A., & Mann, M. (2002) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m200025-mcp200 · Cited by 5,574
Quantitative analysis of complex protein mixtures using isotope-coded affinity tags
Gygi, S. P., Rist, B., Gerber, S. A., Turecek, F., Gelb, M. H., & Aebersold, R. (1999) · Nature Biotechnology · doi:10.1038/13690 · Cited by 4,683 · cited 1×
“Isotope-coded affinity tags (ICAT), thiol-reactive molecules containing light or heavy isotopes, represent one of the earliest-developed strategies (89), and have been successfully utilized for phosphopeptide quantitation (90).”
BackgroundRoss, P. L., Huang, Y. N., Marchese, J. N., Williamson, B., Parker, K., Hattan, S., Khainovski, N., Pillai, S., Dey, S., Daniels, S., et al. (2004) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m400129-mcp200 · Cited by 4,401
Elias, J. E., & Gygi, S. P. (2007) · Nature Methods · doi:10.1038/nmeth1019 · Cited by 3,925
Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry
Syka, J. E. P., Coon, J. J., Schroeder, M. J., Shabanowitz, J., & Hunt, D. F. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0402700101 · Cited by 2,370
Self-citationBeausoleil, S. A., Villén, J., Gerber, S. A., Rush, J., & Gygi, S. P. (2006) · Nature Biotechnology · doi:10.1038/nbt1240 · Cited by 1,577
Large-scale phosphorylation analysis of mouse liver
Villén, J., Beausoleil, S. A., Gerber, S. A., & Gygi, S. P. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0609836104 · Cited by 730
Krüger, M., Moser, M., Ussar, S., Thievessen, I., Luber, C. A., Forner, F., Schmidt, S., Zanivan, S., Fässler, R., & Mann, M. (2008) · Cell · doi:10.1016/j.cell.2008.05.033 · Cited by 680
Daub, H., Olsen, J. V., Bairlein, M., Gnad, F., Oppermann, F. S., Körner, R., Greff, Z., Kéri, G., Stemmann, O., & Mann, M. (2008) · Molecular Cell · doi:10.1016/j.molcel.2008.07.007 · Cited by 594
Metabolic Labeling of Mammalian Organisms with Stable Isotopes for Quantitative Proteomic Analysis
Wu, C. C., MacCoss, M. J., Howell, K. E., Matthews, D. E., & Yates, J. R. (2004) · Analytical Chemistry · doi:10.1021/ac049208j · Cited by 386
Cited by
Hebert, A. S., Richards, A. L., Bailey, D. J., Ulbrich, A., Coughlin, E. E., Westphall, M. S., & Coon, J. J. (2014) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m113.034769 · Cited by 528
Self-citationNesvizhskii, A. I. (2010) · Journal of Proteomics · doi:10.1016/j.jprot.2010.08.009 · Cited by 536
A large-scale method to measure absolute protein phosphorylation stoichiometries
Wu, R., Haas, W., Dephoure, N., Huttlin, E. L., Zhai, B., Sowa, M. E., & Gygi, S. P. (2011) · Nature Methods · doi:10.1038/nmeth.1636 · Cited by 276
Fast and deep phosphoproteome analysis with the Orbitrap Astral mass spectrometer
(2024) · doi:10.1038/s41467-024-51274-0 · Cited by 83
Metadata
- Authors
- Grimsrud, P. A., Swaney, D. L., Wenger, C. D., Beauchene, N. A., & Coon, J. J.
- Venue
- ACS Chemical Biology
- Publisher
- American Chemical Society (ACS)
- Published
- 2010-01-04
- Volume
- 5
- Issue
- 1
- Pages
- 105-119
- ISSN
- 1554-8929 (ISSN-L)
- 1554-8937
- OpenAlex
- W2169544369
- Cited by
- 163
- References
- 11
- FWCI
- 12.71
- Type
- journal article
- Language
- en
- Open access
- green
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- Grimsrud, P. A., Swaney, D. L., Wenger, C. D., Beauchene, N. A., & Coon, J. J. (2010). Phosphoproteomics for the Masses. ACS Chemical Biology. https://doi.org/10.1021/cb900277e
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