The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Gold Nanorod Bioconjugates
Abstract
Gold nanorods have been stabilized, conjugated to antibodies, and characterized for biological applications. The stabilizing surfactant bilayer which surrounds gold nanorods was replaced by thiol terminated methoxypoly(ethylene glycol) so that the nanorods are stable in buffer solutions free of surfactant. Nanorod bioconjugation was accomplished with a heterobifunctional cross-linker, with antibody activity confirmed by a strip plate assay. Nanorod bioconjugates were characterized by independent measurements of the nanorod and antibody concentrations. The nanorod molar extinction coefficient was measured from dense yet well separated nanorod films to be 4.4 ± 0.5 × 10 9 M - 1 cm - 1, thus allowing quantitative determination of nanorod concentrations. However, a colorimetric protein assay overestimated the antibody concentration and is therefore likely perturbed by the presence of the nanorods electromagnetically and/or chemically.
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Cites
Measurement of protein using bicinchoninic acid
Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., & Klenk, D. C. (1985) · Analytical Biochemistry · doi:10.1016/0003-2697(85)90442-7 · Cited by 18,482
A clearer vision for in vivo imaging
Weissleder, R. (2001) · Nature Biotechnology · doi:10.1038/86684 · Cited by 3,839
Cited by
Plasmonic photothermal therapy (PPTT) using gold nanoparticles
Huang, X., Jain, P. K., El-Sayed, I. H., & El-Sayed, M. A. (2008) · Lasers in Medical Science · doi:10.1007/s10103-007-0470-x · Cited by 2,376
Gold Nanorods: From Synthesis and Properties to Biological and Biomedical Applications
Huang, X., Neretina, S., & El‐Sayed, M. A. (2009) · Advanced Materials · doi:10.1002/adma.200802789 · Cited by 1,921
Optical Properties of Star-Shaped Gold Nanoparticles
Nehl, C. L., Liao, H., & Hafner, J. H. (2006) · Nano Letters · doi:10.1021/nl052409y · Cited by 1,162
Self-citationVarious methods of gold nanoparticles (GNPs) conjugation to antibodies
Jazayeri, M. H., Amani, H., Pourfatollah, A. A., Pazoki-Toroudi, H., & Sedighimoghaddam, B. (2016) · Sensing and Bio-Sensing Research · doi:10.1016/j.sbsr.2016.04.002 · Cited by 528
Xu, S., Liu, Y., Wang, T., & Li, J. (2011) · Analytical Chemistry · doi:10.1021/ac200237j · Cited by 367
Metadata
- Authors
- Liao, H., & Hafner, J. H.
- Venue
- Chemistry of Materials
- Publisher
- American Chemical Society (ACS)
- Published
- 2005-08-05
- Volume
- 17
- Issue
- 18
- Pages
- 4636-4641
- ISSN
- 0897-4756 (ISSN-L)
- 1520-5002
- OpenAlex
- W2053194254
- Cited by
- 434
- References
- 2
- FWCI
- 12.06
- Type
- journal article
- Language
- en
- Open access
- closed
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