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Structural Characterization of Proteins with an Attached ATCUN Motif by Paramagnetic Relaxation Enhancement NMR Spectroscopy
Abstract
The use of a short, three-residue Cu(2+)-binding sequence, the ATCUN motif, is presented as an approach for extracting long-range distance restraints from relaxation enhancement NMR spectroscopy. The ATCUN motif is prepended to the N-termini of proteins and binds Cu(2+) with a very high affinity. Relaxation rates of amide protons in ATCUN-tagged protein in the presence and absence of Cu(2+) can be converted into distance restraints and used for structure refinement by using a new routine, PMAG, that has been written for the structure calculation program CNS. The utility of the approach is demonstrated with an application to ATCUN-tagged ubiquitin. Excellent agreement between measured relaxation rates and those calculated on the basis of the X-ray structure of the protein have been obtained.
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Cited by
Visualization of transient encounter complexes in protein–protein association
Tang, C., Iwahara, J., & Clore, G. M. (2006) · Nature · doi:10.1038/nature05201 · Cited by 406
Lee, G. M., Pufall, M. A., Meeker, C. A., Kang, H.-S., Graves, B. J., & McIntosh, L. P. (2008) · Journal of Molecular Biology · doi:10.1016/j.jmb.2008.07.064 · Cited by 58
Metadata
- Authors
- Donaldson, L. W., Skrynnikov, N. R., Choy, W.-Y., Muhandiram, D. R., Sarkar, B., Forman-Kay, J. D., & Kay, L. E.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2001-09-15
- Volume
- 123
- Issue
- 40
- Pages
- 9843-9847
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2046924261
- Cited by
- 175
- References
- 0
- FWCI
- 3.79
- Type
- journal article
- Language
- en
- Open access
- closed
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- The paper
- Donaldson, L. W., Skrynnikov, N. R., Choy, W.-Y., Muhandiram, D. R., Sarkar, B., Forman-Kay, J. D., & Kay, L. E. (2001). Structural Characterization of Proteins with an Attached ATCUN Motif by Paramagnetic Relaxation Enhancement NMR Spectroscopy. Journal of the American Chemical Society. https://doi.org/10.1021/ja011241p
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