Cited by 408Protein Structure and DynamicsDiffusion and Search DynamicsAdvanced NMR Techniques and Applications

Visualization of transient encounter complexes in protein–protein association

Tang, Chun, Iwahara, Junji, Clore, G. Marius·2006·Nature

Abstract

Kinetic data on a number of protein-protein associations have provided evidence for the initial formation of a pre-equilibrium encounter complex that subsequently relaxes to the final stereospecific complex. Site-directed mutagenesis and brownian dynamics simulations have suggested that the rate of association can be modulated by perturbations in charge distribution outside the direct interaction surfaces. Furthermore, rate enhancement through non-specific binding may occur by either a reduction in dimensionality or the presence of a short-range, non-specific attractive potential. Here, using paramagnetic relaxation enhancement, we directly demonstrate the existence and visualize the distribution of an ensemble of transient, non-specific encounter complexes under equilibrium conditions for a relatively weak protein-protein complex between the amino-terminal domain of enzyme I and the phosphocarrier protein HPr. Neither the stereospecific complex alone nor any single alternative conformation can account fully for the intermolecular paramagnetic relaxation enhancement data. Restrained rigid-body simulated annealing refinement against the paramagnetic relaxation enhancement data enables us to obtain an atomic probability distribution map of the non-specific encounter complex ensemble that qualitatively correlates with the electrostatic surface potentials on the interacting proteins. Qualitatively similar results are presented for two other protein-protein complexes.

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Crossref · 10.1038/nature05201

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Authors
Tang, C., Iwahara, J., & Clore, G. M.
Affiliations
National Institutes of Health (US)
Corresponding author
Clore, G. Marius
Venue
Nature
Published
2006-10-15
Volume
444
Issue
7117
Pages
383-386
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
OpenAlex
W2063810968
Cited by
406
References
26
Citations by year
2026: 5 · 2025: 6 · 2024: 5 · 2023: 9 · 2022: 14 · 2021: 10 · 2020: 16 · 2019: 19 · 2018: 15 · 2017: 15 · 2016: 23 · 2015: 28 · 2014: 22 · 2013: 32 · 2012: 30
FWCI
12.09
Type
journal article
Language
en
Open access
closed
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
Funders
  • National Institutes of Health
  • National Institute of Diabetes and Digestive and Kidney Diseases
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Diffusion and Search Dynamics
  • Physical Sciences › Chemistry › Spectroscopy › Advanced NMR Techniques and Applications
Keywords
protein-protein interaction, non-specific binding, paramagnetic relaxation enhancement, surface electrostatic potential
MeSH terms
Bacterial Proteins, Enzymes, Kinetics, Models, Molecular, Phosphoenolpyruvate Sugar Phosphotransferase System, Protein Binding, Protein Conformation, Nuclear Magnetic Resonance, Biomolecular, Multiprotein Complexes, Static Electricity

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