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Activity of Candida rugosa Lipase Immobilized on γ-Fe2O3 Magnetic Nanoparticles
Abstract
We report the stability and enzymatic activity of Candida rugosa Lipase (E.C.3.1.1.3) immobilized on gamma-Fe2O3 magnetic nanoparticles. The immobilization strategies were either reacting the enzyme amine group with a nanoparticle surface acetyl, or amine groups. In the former, the enzyme was attached through a C=N bond, while in the latter it was connected using glutaraldehyde. AFM images show an average particle size of 20 +/- 10 nm after deconvolution. The enzymatic activity of the immobilized lipase was determined by following the ester cleavage of p-nitrophenol butyrate. The covalently immobilized enzyme was stabile and reactive over 30 days.
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Citations
Cited by
Reddy, L. H., Arias, J. L., Nicolas, J., & Couvreur, P. (2012) · Chemical Reviews · doi:10.1021/cr300068p · Cited by 2,010
Characterization of aqueous dispersions of Fe3O4 nanoparticles and their biomedical applications
Cheng, F.-Y., Su, C.-H., Yang, Y.-S., Yeh, C.-S., Tsai, C.-Y., Wu, C.-L., Wu, M.-T., & Shieh, D.-B. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2004.03.016 · Cited by 668
Solvothermal Synthesis and Characterization of Fe3O4 and γ-Fe2O3 Nanoplates
Lu, J., Jiao, X., Chen, D., & Li, W. (2009) · The Journal of Physical Chemistry C · doi:10.1021/jp810583e · Cited by 308
Nitin, N., LaConte, L. E. W., Zurkiya, O., Hu, X., & Bao, G. (2004) · JBIC Journal of Biological Inorganic Chemistry · doi:10.1007/s00775-004-0560-1 · Cited by 295
Immobilization of Trametes versicolor Laccase on Magnetically Separable Mesoporous Silica Spheres
Zhu, Y., Kaskel, S., Shi, J., Wage, T., & van Pée, K.-H. (2007) · Chemistry of Materials · doi:10.1021/cm071265g · Cited by 176
Metadata
- Authors
- Dyal, A., Loos, K., Noto, M., Chang, S. W., Spagnoli, C., Shafi, K. V. P. M., Ulman, A., Cowman, M., & Gross, R. A.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-01-28
- Volume
- 125
- Issue
- 7
- Pages
- 1684-1685
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2254901956
- Cited by
- 554
- References
- 0
- FWCI
- 8.79
- Type
- journal article
- Language
- en
- Open access
- closed
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- Dyal, A., Loos, K., Noto, M., Chang, S. W., Spagnoli, C., Shafi, K. V. P. M., Ulman, A., Cowman, M., & Gross, R. A. (2003). Activity of Candida rugosa Lipase Immobilized on γ-Fe2O3 Magnetic Nanoparticles. Journal of the American Chemical Society. https://doi.org/10.1021/ja021223n
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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