Characterization of aqueous dispersions of Fe3O4 nanoparticles and their biomedical applications
Abstract
A newly developed non-polymer coated Fe(3)O(4) nanoparticles showing well-dispersion were synthesized using Fe(II) and Fe(III) salt chemical coprecipitation with tetramethylammonium hydroxide (N(CH(3))(4)OH) in an aqueous solution. Transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectrometer (FT-IR), X-ray photoelectron spectrometer (XPS) and superconducting quantum interference measurement device (SQUID) measurements were employed to investigate the iron oxide properties. The resulting iron oxide particles were manipulated to be as small as 9 nm diameter in size. Based on FT-IR and X-ray photoelectron spectrometer results, it is suggested that the surfaces of the magnetite (Fe(3)O(4)) particles are covered with hydroxide (-OH) groups incorporated with (CH(3))(4)N(+) through electrostatic interaction. The in vitro cytotoxicity test revealed that the magnetite particles exhibited excellent biocompatibility, suggesting that they may be further explored for biomedical applications. NMR measurements revealed significantly reduced water proton relaxation times T1 and T2. The MR images of the nanoparticles in water, serum, and whole blood were investigated using a 1.5 T clinical MR imager. Significant reduction of the background medium signal was achieved in the T2-weighted and the T2*-weighted sequence especially in the serum and whole blood. Combining the advantage of MRI signal contrast, the non-polymer-coated surface chemistry for distinct bioconjugation and the homogenous nanometer size for better controlled biodistribution, these preliminary experiments demonstrated the potential of the as-synthesized magnetite material in functional molecular imaging for biomedical research and clinical diagnosis.
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Upon surface modification, superparamagnetic iron oxide nanoparticles (SPION) can be employed for magnetic resonance imaging, magnetic particle imaging, targeted delivery of drugs, proteins, antibodies and nucleic acids, hyperthermia, biosensing, tissue repair, and separation of biomolecules.
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From Iron oxide nanoparticles: Diagnostic, therapeutic and theranostic applications (2019) · cites this paper as [5]
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Iron oxide nanoparticles: Diagnostic, therapeutic and theranostic applications
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- Authors
- Cheng, F.-Y., Su, C.-H., Yang, Y.-S., Yeh, C.-S., Tsai, C.-Y., Wu, C.-L., Wu, M.-T., & Shieh, D.-B.
- Affiliations
- National Cheng Kung University (TW)
- Kaohsiung Veterans General Hospital (TW)
- Corresponding author
- Yeh, Chen-Sheng
- Wu, Chao-Liang
- Shieh, Dar-Bin
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2005-03
- Volume
- 26
- Issue
- 7
- Pages
- 729-738
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W2084797392
- Cited by
- 668
- References
- 46
- Citations by year
- 2026: 8 · 2025: 16 · 2024: 9 · 2023: 24 · 2022: 19 · 2021: 25 · 2020: 32 · 2019: 33 · 2018: 37 · 2017: 37 · 2016: 34 · 2015: 35 · 2014: 46 · 2013: 45 · 2012: 38
- FWCI
- 13.68
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Council
- National Cheng Kung University
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Characterization and Applications of Magnetic Nanoparticles
- Physical Sciences › Materials Science › Materials Chemistry › Magnetic Properties and Synthesis of Ferrites
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
- Keywords
- Fe3O4 nanoparticles, magnetite, aqueous dispersions, chemical coprecipitation, tetramethylammonium hydroxide, biocompatibility, cytotoxicity, MRI contrast agents, T2-weighted imaging, tissue distribution, molecular imaging
- MeSH terms
- Animals, Biocompatible Materials, Biomedical Engineering, Chlorocebus aethiops, Colloids, Ferric Compounds, Hemolysis, Humans, Materials Testing, Particle Size, Water, COS Cells, Nanotubes
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