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An Efficient RNA-Cleaving DNA Enzyme that Synchronizes Catalysis with Fluorescence Signaling
Abstract
DNA enzymes are single-stranded DNA molecules with catalytic capabilities that are isolated from random-sequence DNA libraries by "in vitro selection". This new class of catalytic biomolecules has the potential of being used as unique molecular tools in a variety of innovative applications. Here we describe the creation and characterization of an RNA-cleaving autocatalytic DNA, DEC22-18, that uniquely links chemical catalysis with real-time fluorescence signaling capability in the same molecule. A trans-acting DNA molecule, DET22-18, was also developed from DEC22-18 that behaves as a true enzyme with a k(cat) of approximately 7 min(-1)-a rate constant that is the second largest ever reported for a DNA enzyme. It cleaves a chimeric RNA/DNA substrate at the lone RNA linkage surrounded by a closely spaced fluorophore-quencher pair-a unique structure that permits the synchronization of the chemical cleavage with fluorescence signaling. DET22-18 has a stem-loop structure and can be conjugated with DNA aptamers to form allosteric deoxyribozyme biosensors.
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Cited by
Zhou, W., Saran, R., & Liu, J. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00063 · Cited by 931
McConnell, E. M., Cozma, I., Mou, Q., Brennan, J. D., Lu, Y., & Li, Y. (2021) · Chemical Society Reviews · doi:10.1039/d1cs00240f · Cited by 477
Self-citationXiang, Y., Tong, A., & Lu, Y. (2009) · Journal of the American Chemical Society · doi:10.1021/ja905854a · Cited by 335
DNAzyme-based biosensors and nanodevices
Gong, L., Zhao, Z., Lv, Y.-F., Huan, S.-Y., Fu, T., Zhang, X.-B., Shen, G.-L., & Yu, R.-Q. (2015) · Chemical Communications · doi:10.1039/c4cc06855f · Cited by 321
Electrochemical DNAzyme Sensor for Lead Based on Amplification of DNA−Au Bio-Bar Codes
Shen, L., Chen, Z., Li, Y., He, S., Xie, S., Xu, X., Liang, Z., Meng, X., Li, Q., Zhu, Z., et al. (2008) · Analytical Chemistry · doi:10.1021/ac800601y · Cited by 278
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Metadata
- Authors
- Mei, S. H. J., Liu, Z., Brennan, J. D., & Li, Y.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2002-12-13
- Volume
- 125
- Issue
- 2
- Pages
- 412-420
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W1982237778
- Cited by
- 188
- References
- 0
- FWCI
- 2.05
- Type
- journal article
- Language
- en
- Open access
- closed
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