The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Cross-Correlated Relaxation Enhanced 1H−13C NMR Spectroscopy of Methyl Groups in Very High Molecular Weight Proteins and Protein Complexes
Abstract
A comparison of HSQC and HMQC pulse schemes for recording (1)H[bond](13)C correlation maps of protonated methyl groups in highly deuterated proteins is presented. It is shown that HMQC correlation maps can be as much as a factor of 3 more sensitive than their HSQC counterparts and that the sensitivity gains result from a TROSY effect that involves cancellation of intra-methyl dipolar relaxation interactions. (1)H[bond](13)C correlation spectra are recorded on U-[(15)N,(2)H], Ile delta 1-[(13)C,(1)H] samples of (i) malate synthase G, a 723 residue protein, at 37 and 5 degrees C, and of (ii) the protease ClpP, comprising 14 identical subunits, each with 193 residues (305 kDa), at 5 degrees C. The high quality of HMQC spectra obtained in short measuring times strongly suggests that methyl groups will be useful probes of structure and dynamics in supramolecular complexes.
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Citations
Cites
Principles of Nuclear Magnetic Resonance in One and Two Dimensions
Ernst, R. R., Bodenhausen, G., & Wokaun, A. (1990) · doi:10.1093/oso/9780198556473.001.0001 · Cited by 1,418
Cited by
Schanda, P., Kupče, Ē., & Brutscher, B. (2005) · Journal of Biomolecular NMR · doi:10.1007/s10858-005-4425-x · Cited by 709
Quantitative dynamics and binding studies of the 20S proteasome by NMR
Sprangers, R., & Kay, L. E. (2007) · Nature · doi:10.1038/nature05512 · Cited by 527
Self-citationHsp90-Tau complex reveals molecular basis for specificity in chaperone action
Karagöz, G. E., Duarte, A. M. S., Akoury, E., Ippel, H., Biernat, J., Morán Luengo, T., Radli, M., Didenko, T., Nordhues, B. A., Veprintsev, D. B., et al. (2014) · Cell · doi:10.1016/j.cell.2014.01.037 · Cited by 318
“Spectra processing, analysis, and representations were done as described (Karagöz et al., 2011; Tugarinov et al., 2003).”
MethodHistones: At the Crossroads of Peptide and Protein Chemistry
Müller, M. M., & Muir, T. W. (2015) · Chemical Reviews · doi:10.1021/cr5003529 · Cited by 247
Structural and Energetic Mechanisms of Cooperative Autoinhibition and Activation of Vav1
Yu, B., Martins, I. R. S., Li, P., Amarasinghe, G. K., Umetani, J., Fernandez-Zapico, M. E., Billadeau, D. D., Machius, M., Tomchick, D. R., & Rosen, M. K. (2010) · Cell · doi:10.1016/j.cell.2009.12.033 · Cited by 156
Metadata
- Authors
- Tugarinov, V., Hwang, P. M., Ollerenshaw, J. E., & Kay, L. E.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-08-01
- Volume
- 125
- Issue
- 34
- Pages
- 10420-10428
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2074856155
- Cited by
- 649
- References
- 1
- FWCI
- 4.85
- Type
- journal article
- Language
- en
- Open access
- closed
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