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Base-Dependent Competitive Adsorption of Single-Stranded DNA on Gold
Abstract
We characterize the room-temperature adsorption of single-stranded DNA homo-oligonucleotides from solution onto polycrystalline Au films, including competitive adsorption between all possible pairs of unmodified oligomers. Fourier transform infrared (FTIR) and X-ray photoelectron (XPS) spectroscopy analysis of the resulting films shows that oligonucleotides adsorb with a strongly base-dependent affinity, adenine (A) > cytosine (C) >/= guanine (G) > thymine (T). In competitive adsorption experiments on Au, oligo(dA) strongly dominates over the other oligonucleotides. The relative adsorption affinity of oligo(dA) is so great that it competes effectively against adsorption of thiolated oligomers and even causes hybridized oligo(dA).oligo(dT) duplexes to denature in the presence of Au.
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Citations
Cited by
Electrochemistry of Nucleic Acids
Paleček, E., & Bartošík, M. (2012) · Chemical Reviews · doi:10.1021/cr200303p · Cited by 668
A gold nanoparticle-based chronocoulometric DNA sensor for amplified detection of DNA
Zhang, J., Song, S., Wang, L., Pan, D., & Fan, C. (2007) · Nature Protocols · doi:10.1038/nprot.2007.419 · Cited by 473
Functional DNA Nanostructures for Theranostic Applications
Pei, H., Zuo, X., Zhu, D., Huang, Q., & Fan, C. (2014) · Accounts of Chemical Research · doi:10.1021/ar400195t · Cited by 409
Adsorption of DNA onto gold nanoparticles and graphene oxide: surface science and applications
Liu, J. (2012) · Physical Chemistry Chemical Physics · doi:10.1039/c2cp41186e · Cited by 408
Metadata
- Authors
- Kimura-Suda, H., Petrovykh, D. Y., Tarlov, M. J., & Whitman, L. J.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-07-01
- Volume
- 125
- Issue
- 30
- Pages
- 9014-9015
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2053014859
- Cited by
- 488
- References
- 0
- FWCI
- 3.22
- Type
- journal article
- Language
- en
- Open access
- closed
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- Kimura-Suda, H., Petrovykh, D. Y., Tarlov, M. J., & Whitman, L. J. (2003). Base-Dependent Competitive Adsorption of Single-Stranded DNA on Gold. Journal of the American Chemical Society. https://doi.org/10.1021/ja035756n
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