The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Detecting Protein−Protein Interactions with a Green Fluorescent Protein Fragment Reassembly Trap: Scope and Mechanism
Abstract
Identification of protein binding partners is one of the key challenges of proteomics. We recently introduced a screen for detecting protein-protein interactions based on reassembly of dissected fragments of green fluorescent protein fused to interacting peptides. Here, we present a set of comaintained Escherichia coli plasmids for the facile subcloning of fusions to the green fluorescent protein fragments. Using a library of antiparallel leucine zippers, we have shown that the screen can detect very weak interactions (K(D) approximately 1 mM). In vitro kinetics show that the reassembly reaction is essentially irreversible, suggesting that the screen may be useful for detecting transient interactions. Finally, we used the screen to discriminate cognate from noncognate protein-ligand interactions for tetratricopeptide repeat domains. These experiments demonstrate the general utility of the screen for larger proteins and elucidate mechanistic details to guide the further use of this screen in proteomic analysis. Additionally, this work gives insight into the positional inequivalence of stabilizing interactions in antiparallel coiled coils.
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Tsien, R. Y. (1998) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.67.1.509 · Cited by 6,143
A novel genetic system to detect protein–protein interactions
Fields, S., & Song, O.-K. (1989) · Nature · doi:10.1038/340245a0 · Cited by 6,114
Global Analysis of Protein Activities Using Proteome Chips
Zhu, H., Bilgin, M., Bangham, R., Hall, D., Casamayor, A., Bertone, P., Lan, N., Jansen, R., Bidlingmaier, S., Houfek, T., et al. (2001) · Science · doi:10.1126/science.1062191 · Cited by 1,884
Structure and Mechanism in Protein Science. A guide to Enzyme Catalysis and Protein Folding
Howland, J. L. (2001) · Biochemistry and Molecular Biology Education · Cited by 1,823
The Tandem Affinity Purification (TAP) Method: A General Procedure of Protein Complex Purification
Puig, O., Caspary, F., Rigaut, G., Rutz, B., Bouveret, E., Bragado-Nilsson, E., Wilm, M., & Séraphin, B. (2001) · Methods · doi:10.1006/meth.2001.1183 · Cited by 1,783
Hu, C.-D., Chinenov, Y., & Kerppola, T. K. (2002) · Molecular Cell · doi:10.1016/s1097-2765(02)00496-3 · Cited by 1,549
Structure of TPR Domain–Peptide Complexes
Scheufler, C., Brinker, A., Bourenkov, G., Pegoraro, S., Moroder, L., Bartunik, H., Hartl, F. U., & Moarefi, I. (2000) · Cell · doi:10.1016/s0092-8674(00)80830-2 · Cited by 1,188
Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling
Crameri, A., Whitehorn, E. A., Tate, E., & Stemmer, W. P. C. (1996) · Nature Biotechnology · doi:10.1038/nbt0396-315 · Cited by 1,187
Coiled coils: new structures and new functions
LUPAS, A. (1996) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(96)10052-9 · Cited by 1,183
TPR proteins: the versatile helix
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NanoLuc Complementation Reporter Optimized for Accurate Measurement of Protein Interactions in Cells
Dixon, A. S., Schwinn, M. K., Hall, M. P., Zimmerman, K., Otto, P., Lubben, T. H., Butler, B. L., Binkowski, B. F., Machleidt, T., Kirkland, T. A., et al. (2016) · ACS Chemical Biology · doi:10.1021/acschembio.5b00753 · Cited by 1,405
LAMTOR/Ragulator is a negative regulator of Arl8b- and BORC-dependent late endosomal positioning
Filipek, P. A., de Araujo, M. E. G., Vogel, G. F., De Smet, C. H., Eberharter, D., Rebsamen, M., Rudashevskaya, E. L., Kremser, L., Yordanov, T., Tschaikner, P., et al. (2017) · Journal of Cell Biology · doi:10.1083/jcb.201703061 · Cited by 119
Bottone, S., Joliot, O., Cakil, Z. V., El Hajji, L., Rakotoarison, L.-M., Boncompain, G., Perez, F., & Gautier, A. (2023) · Nature Methods · doi:10.1038/s41592-023-01988-8 · Cited by 48
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Metadata
- Authors
- Magliery, T. J., Wilson, C. G. M., Pan, W., Mishler, D., Ghosh, I., Hamilton, A. D., & Regan, L.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2004-12-02
- Volume
- 127
- Issue
- 1
- Pages
- 146-157
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2019248292
- Cited by
- 441
- References
- 33
- FWCI
- 9.68
- Type
- journal article
- Language
- en
- Open access
- closed
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- Magliery, T. J., Wilson, C. G. M., Pan, W., Mishler, D., Ghosh, I., Hamilton, A. D., & Regan, L. (2005). Detecting Protein−Protein Interactions with a Green Fluorescent Protein Fragment Reassembly Trap: Scope and Mechanism. Journal of the American Chemical Society. https://doi.org/10.1021/ja046699g
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