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Labeling Proteins with Small Molecules by Site-Specific Posttranslational Modification
Abstract
We report here the development of a general strategy for site-specific labeling of proteins with small molecules by posttranslational modification enzyme, phosphopantetheinyl transferase Sfp. The target proteins are expressed as fusions to the peptide carrier protein (PCP) excised from nonribosomal peptide synthetase, and Sfp catalyzes the covalent modification of a specific serine residue on PCP by the small molecule-phosphopantetheinyl conjugate. The labeling reaction proceeds with high specificity and efficiency, targeting PCP fusion proteins in the cell lysate. The PCP tag has been shown to be compatible with various proteins, and Sfp-catalyzed PCP modification, compatible with various small-molecule probes conjugated to coenzyme A, highlighting the potential of the PCP tag for site-specific protein labeling with small molecules.
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Quadri, L. E. N., Weinreb, P. H., Lei, M., Nakano, M. M., Zuber, P., & Walsh, C. T. (1998) · Biochemistry · doi:10.1021/bi9719861 · Cited by 693
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Prescher, J. A., & Bertozzi, C. R. (2005) · Nature Chemical Biology · doi:10.1038/nchembio0605-13 · Cited by 1,456
Cellular Incorporation of Unnatural Amino Acids and Bioorthogonal Labeling of Proteins
Lang, K., & Chin, J. W. (2014) · Chemical Reviews · doi:10.1021/cr400355w · Cited by 1,035
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Chen, I., Howarth, M., Lin, W., & Ting, A. Y. (2005) · Nature Methods · doi:10.1038/nmeth735 · Cited by 667
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Metadata
- Authors
- Yin, J., Liu, F., Li, X., & Walsh, C. T.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2004-06-01
- Volume
- 126
- Issue
- 25
- Pages
- 7754-7755
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2070858181
- Cited by
- 200
- References
- 1
- FWCI
- 11.65
- Type
- journal article
- Language
- en
- Open access
- closed
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- Yin, J., Liu, F., Li, X., & Walsh, C. T. (2004). Labeling Proteins with Small Molecules by Site-Specific Posttranslational Modification. Journal of the American Chemical Society. https://doi.org/10.1021/ja047749k
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