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Two Separate Gene Clusters Encode the Biosynthetic Pathway for the Meroterpenoids Austinol and Dehydroaustinol in Aspergillus nidulans
Open accessAbstract
Meroterpenoids are a class of fungal natural products that are produced from polyketide and terpenoid precursors. An understanding of meroterpenoid biosynthesis at the genetic level should facilitate engineering of second-generation molecules and increasing production of first-generation compounds. The filamentous fungus Aspergillus nidulans has previously been found to produce two meroterpenoids, austinol and dehydroaustinol. Using targeted deletions that we created, we have determined that, surprisingly, two separate gene clusters are required for meroterpenoid biosynthesis. One is a cluster of four genes including a polyketide synthase gene, ausA. The second is a cluster of 10 additional genes including a prenyltransferase gene, ausN, located on a separate chromosome. Chemical analysis of mutant extracts enabled us to isolate 3,5-dimethylorsellinic acid and 10 additional meroterpenoids that are either intermediates or shunt products from the biosynthetic pathway. Six of them were identified as novel meroterpenoids in this study. Our data, in aggregate, allow us to propose a complete biosynthetic pathway for the A. nidulans meroterpenoids.
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Cited by
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de Vries, R. P., Riley, R., Wiebenga, A., Aguilar-Osorio, G., Amillis, S., Uchima, C. A., Anderluh, G., Asadollahi, M., Askin, M., Barry, K., et al. (2017) · Genome Biology · doi:10.1186/s13059-017-1151-0 · Cited by 489
Self-citationOxidative Cyclization in Natural Product Biosynthesis
Tang, M.-C., Zou, Y., Watanabe, K., Walsh, C. T., & Tang, Y. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00478 · Cited by 425
Inglis, D. O., Binkley, J., Skrzypek, M. S., Arnaud, M. B., Cerqueira, G. C., Shah, P., Wymore, F., Wortman, J. R., & Sherlock, G. (2013) · BMC Microbiology · doi:10.1186/1471-2180-13-91 · Cited by 293
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Metadata
- Authors
- Lo, H.-C., Entwistle, R., Guo, C.-J., Ahuja, M., Szewczyk, E., Hung, J.-H., Chiang, Y.-M., Oakley, B. R., & Wang, C. C. C.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2012-02-29
- Volume
- 134
- Issue
- 10
- Pages
- 4709-4720
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2059861223
- Cited by
- 250
- References
- 0
- FWCI
- 13.83
- Type
- journal article
- Language
- en
- Open access
- green
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- Lo, H.-C., Entwistle, R., Guo, C.-J., Ahuja, M., Szewczyk, E., Hung, J.-H., Chiang, Y.-M., Oakley, B. R., & Wang, C. C. C. (2012). Two Separate Gene Clusters Encode the Biosynthetic Pathway for the Meroterpenoids Austinol and Dehydroaustinol in Aspergillus nidulans. Journal of the American Chemical Society. https://doi.org/10.1021/ja209809t
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