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A Genetically Encoded Fluorescent Probe in Mammalian Cells
Open accessAbstract
Fluorescent reporters are useful in vitro and in vivo probes of protein structure, function, and localization. Here we report that the fluorescent amino acid, 3-(6-acetylnaphthalen-2-ylamino)-2-aminopropanoic acid (Anap), can be site-specifically incorporated into proteins in mammalian cells in response to the TAG codon with high efficiency using an orthogonal amber suppressor tRNA/aminoacyl-tRNA synthetase (aaRS) pair. We further demonstrate that Anap can be used to image the subcellular localization of proteins in live mammalian cells. The small size of Anap, its environment-sensitive fluorescence, and the ability to introduce Anap at specific sites in the proteome by simple mutagenesis make it a unique and valuable tool in eukaryotic cell biology.
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Citations
Cited by
Cellular Incorporation of Unnatural Amino Acids and Bioorthogonal Labeling of Proteins
Lang, K., & Chin, J. W. (2014) · Chemical Reviews · doi:10.1021/cr400355w · Cited by 1,035
Wan, Q., Chen, S., Shi, W., Li, L., & Ma, H. (2014) · Angewandte Chemie International Edition · doi:10.1002/anie.201405742 · Cited by 420
Labeling and Single-Molecule Methods To Monitor G Protein-Coupled Receptor Dynamics
Tian, H., Fürstenberg, A., & Huber, T. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00084 · Cited by 114
Metadata
- Authors
- Chatterjee, A., Guo, J., Lee, H. S., & Schultz, P. G.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2013-08-14
- Volume
- 135
- Issue
- 34
- Pages
- 12540-12543
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W1985650731
- Cited by
- 228
- References
- 0
- FWCI
- 7.59
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: A Genetically Encoded Fluorescent Probe in Mammalian Cells. https://app.cochat.ai/papers/10.1021/ja4059553
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- Chatterjee, A., Guo, J., Lee, H. S., & Schultz, P. G. (2013). A Genetically Encoded Fluorescent Probe in Mammalian Cells. Journal of the American Chemical Society. https://doi.org/10.1021/ja4059553
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