The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
A Photoactivatable Push−Pull Fluorophore for Single-Molecule Imaging in Live Cells
Open accessAbstract
We have reengineered a red-emitting dicyanomethylenedihydrofuran push-pull fluorophore so that it is dark until photoactivated with a short burst of low-intensity violet light. Photoactivation of the dark fluorogen leads to conversion of an azide to an amine, which shifts the absorption to long wavelengths. After photoactivation, the fluorophore is bright and photostable enough to be imaged on the single-molecule level in living cells. This proof-of-principle demonstration provides a new class of bright photoactivatable fluorophores, as are needed for super-resolution imaging schemes that require active control of single molecule emission.
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Cites
Precise Nanometer Localization Analysis for Individual Fluorescent Probes
Thompson, R. E., Larson, D. R., & Webb, W. W. (2002) · Biophysical Journal · doi:10.1016/s0006-3495(02)75618-x · Cited by 2,320
Cited by
Fluorescent probes for super-resolution imaging in living cells
Fernández-Suárez, M., & Ting, A. Y. (2008) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2531 · Cited by 1,306
Direct stochastic optical reconstruction microscopy with standard fluorescent probes
van de Linde, S., Löschberger, A., Klein, T., Heidbreder, M., Wolter, S., Heilemann, M., & Sauer, M. (2011) · Nature Protocols · doi:10.1038/nprot.2011.336 · Cited by 1,071
Pavani, S. R. P., Thompson, M. A., Biteen, J. S., Lord, S. J., Liu, N., Twieg, R. J., Piestun, R., & Moerner, W. E. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0900245106 · Cited by 1,040
Self-citationSwitchable Fluorophores for Single-Molecule Localization Microscopy
Li, H., & Vaughan, J. C. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00767 · Cited by 319
Kim, E.-J., Kumar, R., Sharma, A., Yoon, B., Kim, H. M., Lee, H., Hong, K. S., & Kim, J. S. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.01.009 · Cited by 129
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Metadata
- Authors
- Lord, S. J., Conley, N. R., Lee, H.-L. D., Samuel, R., Liu, N., Twieg, R. J., & Moerner, W. E.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2008-06-24
- Volume
- 130
- Issue
- 29
- Pages
- 9204-9205
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W1982019125
- Cited by
- 216
- References
- 1
- FWCI
- 9.00
- Type
- journal article
- Language
- en
- Open access
- green
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- Lord, S. J., Conley, N. R., Lee, H.-L. D., Samuel, R., Liu, N., Twieg, R. J., & Moerner, W. E. (2008). A Photoactivatable Push−Pull Fluorophore for Single-Molecule Imaging in Live Cells. Journal of the American Chemical Society. https://doi.org/10.1021/ja802883k
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