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Membrane Proteins Solubilized Intact in Lipid Containing Nanoparticles Bounded by Styrene Maleic Acid Copolymer
Abstract
One-third of eukaryotic proteins are integrated within membranes, as are the targets of 40% of approved drugs. However, the lack of a general means of solubilizing, stabilizing and structurally characterizing active membrane proteins has frustrated efforts to understand their mechanisms and exploit their potential value. Here we report that bilayer disks formed by phospholipids and styrene maleic anhydride copolymer preserve the functional and structural integrity of alpha-helical and beta-barrel transmembrane proteins. They form 11 nm particles that are monodispersed, biocompatible, thermostable, and water-soluble, allowing diverse membrane proteins to be simply and rapidly presented for virtually any in vitro analysis.
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Cited by
Wang, G., Zhou, L., Zhang, P., Zhao, E., Zhou, L., Chen, D., Sun, J., Gu, X., Yang, W., & Tang, B. Z. (2020) · Angewandte Chemie International Edition · doi:10.1002/anie.201913847 · Cited by 70
Membrane protein insertion and assembly by the bacterial holo-translocon SecYEG-SecDF-YajC-YidC.
Komar, J., Alvira, S., Schulze, R. J., Martin, R., Lycklama a Nijeholt, J. A., Lee, S. C., Dafforn, T. R., Deckers-Hebestreit, G., Berger, I., Schaffitzel, C., et al. (2016) · Biochemical Journal · doi:10.1042/bcj20160545 · Cited by 69
Self-citationNano-scale resolution of native retinal rod disk membranes reveals differences in lipid composition
Sander, C. L., Sears, A. E., Pinto, A. F. M., Choi, E. H., Kahremany, S., Gao, F., Salom, D., Jin, H., Pardon, E., Suh, S., et al. (2021) · Journal of Cell Biology · doi:10.1083/jcb.202101063 · Cited by 36
Mazal, H., Wieser, F.-F., Bollschweiler, D., Schambony, A., & Sandoghdar, V. (2025) · Science Advances · doi:10.1126/sciadv.adw4402 · Cited by 17
Cryo‐EM structure of the octameric pore of Clostridium perfringens β‐toxin
Bruggisser, J., Iacovache, I., Musson, S. C., Degiacomi, M. T., Posthaus, H., & Zuber, B. (2022) · The EMBO Reports · doi:10.15252/embr.202254856 · Cited by 13
Metadata
- Authors
- Knowles, T. J., Finka, R., Smith, C., Lin, Y.-P., Dafforn, T., & Overduin, M.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2009-05-18
- Volume
- 131
- Issue
- 22
- Pages
- 7484-7485
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2024900979
- Cited by
- 550
- References
- 0
- FWCI
- 1.56
- Type
- journal article
- Language
- en
- Open access
- closed
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- Knowles, T. J., Finka, R., Smith, C., Lin, Y.-P., Dafforn, T., & Overduin, M. (2009). Membrane Proteins Solubilized Intact in Lipid Containing Nanoparticles Bounded by Styrene Maleic Acid Copolymer. Journal of the American Chemical Society. https://doi.org/10.1021/ja810046q
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