The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Oxidation of Guanine in DNA by Ru(phen)2(dppz)3+ Using the Flash-Quench Technique
Abstract
A flash-quench method has been developed to probe oxidative damage to DNA. A photoexcited Ru(II) intercalator is quenched in DNA by a weakly bound, electron-transfer quencher to generate Ru(III), a powerful ground-state oxidant. Once generated, Δ-Ru(phen) 2 (dppz) 3+ bound to poly(dG-dC) rapidly oxidizes guanine within the DNA duplex. Transient absorption spectroscopy indicates rapid formation of the neutral guanine radical within the DNA duplex. Permanent damage resulting from the flash-quench experiment is monitored by gel electrophoresis of synthetic oligonucleotide duplexes. Oxidative damage, visualized by treatment with piperidine, occurs selectively at the 5‘-G of 5‘-GG-3‘ sites and at the 5‘- and central G of 5‘-GGG-3‘ triplets; enzymatic digestion in the absence of piperidine treatment shows formation of 8-oxo-2‘-deoxyguanosine with Ru(NH 3 ) 6 3+ as quencher. The yield of base damage is, furthermore, modulated by the choice of electron-transfer quencher. Quantum yields for damage vary in the order Ru(NH 3 ) 6 3+ < methyl viologen 2+ < Co(NH 3 ) 5 Cl 2+ and correlate with the instability of the reduced quencher. The flash-quench method, combining spectroscopy and product analysis, offers a novel and tunable approach to explore electron transfer chemistry on double helical DNA.
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Citations
Cites
Insertion of specific bases during DNA synthesis past the oxidation-damaged base 8-oxodG
Shibutani, S., Takeshita, M., & Grollman, A. P. (1991) · Nature · doi:10.1038/349431a0 · Cited by 2,224
Oxidative DNA damage through long-range electron transfer
Hall, D. B., Holmlin, R. E., & Barton, J. K. (1996) · Nature · doi:10.1038/382731a0 · Cited by 825
Self-citation
Cited by
Stubbe, J., Nocera, D. G., Yee, C. S., & Chang, M. C. Y. (2003) · Chemical Reviews · doi:10.1021/cr020421u · Cited by 823
Mechanisms for DNA Charge Transport
Genereux, J. C., & Barton, J. K. (2010) · Chemical Reviews · doi:10.1021/cr900228f · Cited by 787
Self-citationMelatonin and its metabolites vs oxidative stress: From individual actions to collective protection
Galano, A., & Reiter, R. J. (2018) · Journal of Pineal Research · doi:10.1111/jpi.12514 · Cited by 353
Electronic Coupling for Charge Transfer and Transport in DNA
Voityuk, A. A., Rösch, N., Bixon, M., & Jortner, J. (2000) · The Journal of Physical Chemistry B · doi:10.1021/jp001109w · Cited by 271
DNA−Protein Cross-Linking from Oxidation of Guanine via the Flash−Quench Technique
Nguyen, K. L., Steryo, M., Kurbanyan, K., Nowitzki, K. M., Butterfield, S. M., Ward, S. R., & Stemp, E. D. A. (2000) · Journal of the American Chemical Society · doi:10.1021/ja993502p · Cited by 59
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Metadata
- Authors
- Stemp, E. D. A., Arkin, M. R., & Barton, J. K.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 1997-03-01
- Volume
- 119
- Issue
- 12
- Pages
- 2921-2925
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2026084209
- Cited by
- 212
- References
- 2
- FWCI
- 5.06
- Type
- journal article
- Language
- en
- Open access
- closed
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