The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Efforts toward the Expansion of the Genetic Alphabet: Information Storage and Replication with Unnatural Hydrophobic Base Pairs
Abstract
The faithful recognition of the interstrand hydrogen bonds between complementary nucleobases forms the foundation of the genetic code. The ability to replicate DNA containing a stable third base pair would allow for an expansion of the information content of DNA by supplementing the existing two base pairs of the genetic alphabet with a third. We report the optimization of unnatural nucleobases whose pairing in duplex DNA is based on interbase hydrophobic interactions. We show that the stability and selectivity of such unnatural base pairs may be comparable to, or even exceed, that of native pairs. We also demonstrate that several unnatural base pairs are incorporated into DNA by Klenow fragment of Escherichia coli DNA polymerase I with an efficiency equivalent to that of native DNA synthesis. Moreover, the unnatural bases are orthogonal to the native bases, with correct pairing being favored by at least an order of magnitude relative to mispairing.
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Cites
Biochemical Basis of DNA Replication Fidelity
Goodman, M. F., Creighton, S., Bloom, L. B., Petruska, J., & Kunkel, T. A. (1993) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409239309086792 · Cited by 446
Cited by
C-Nucleosides: Synthetic Strategies and Biological Applications
Hocek, M. (2009) · Chemical Reviews · doi:10.1021/cr9002165 · Cited by 383
Detection and beyond: challenges and advances in aptamer-based biosensors
Yoo, H., Jo, H., & Oh, S. S. (2020) · Materials Advances · doi:10.1039/d0ma00639d · Cited by 334
Beyond A, C, G and T: augmenting nature’s alphabet
Henry, A. A., & Romesberg, F. E. (2003) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2003.10.011 · Cited by 276
Self-citationReprogramming the assembly of unmodified DNA with a small molecule
Avakyan, N., Greschner, A. A., Aldaye, F., Serpell, C. J., Toader, V., Petitjean, A., & Sleiman, H. F. (2016) · Nature Chemistry · doi:10.1038/nchem.2451 · Cited by 148
Origins of life: a route to nanotechnology
Sowerby, S. J., Holm, N. G., & Petersen, G. B. (2001) · Biosystems · doi:10.1016/s0303-2647(01)00130-7 · Cited by 34
Metadata
- Authors
- Ogawa, A. K., Wu, Y., McMinn, D. L., Liu, J., Schultz, P. G., & Romesberg, F. E.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2000-03-24
- Volume
- 122
- Issue
- 14
- Pages
- 3274-3287
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2016066488
- Cited by
- 162
- References
- 1
- FWCI
- 5.29
- Type
- journal article
- Language
- en
- Open access
- closed
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