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Cited by 208

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Spectrally Resolved, Functional Super-Resolution Microscopy Reveals Nanoscale Compositional Heterogeneity in Live-Cell Membranes

Moon, Seonah, Yan, Rui, Kenny, Samuel J., Shyu, Yennie, Xiang, Limin, Li, Wan, Xu, Ke·2017·Journal of the American Chemical Society
DOI 10.1021/jacs.7b03846
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Abstract

By recording the full fluorescence spectra and super-resolved positions of ∼10 6 individual polarity-sensing solvatochromic molecules, we reveal compositional heterogeneity in the membranes of live mammalian cells with single-molecule sensitivity and ∼30 nm spatial resolution. This allowed us to unveil distinct polarity characteristics of the plasma membrane and the membranes of nanoscale intracellular organelles, a result we found to be due to differences in cholesterol levels. Within the plasma membrane, we observed the formation of low-polarity, raft-like nanodomains upon cholesterol addition or cholera-toxin treatment, but found this nanoscale phase separation absent in native cells. The ultimate sensitivity achieved through examining the spectra of individual molecules thus opens the door to functional interrogations of intracellular physicochemical parameters at the nanoscale.

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Crossref · 10.1021/jacs.7b03846

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Research works printed but not linked: 37

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Metadata

Authors
Moon, S., Yan, R., Kenny, S. J., Shyu, Y., Xiang, L., Li, W., & Xu, K.
Affiliations
Department of Chemistry, University of California, Berkeley, Berkeley, California 94720, United States
ORCID
Venue
Journal of the American Chemical Society
Publisher
American Chemical Society (ACS)
Published
2017-08-07
Volume
139
Issue
32
Pages
10944-10947
ISSN
  • 0002-7863
  • 1520-5126
DOI
10.1021/jacs.7b03846
Cited by
208
References
2
Language
en
Funders
  • Division of Chemistry (CHE-1554717)
  • Samsung
  • David and Lucile Packard Foundation
  • Arnold and Mabel Beckman Foundation

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