The DOI matches its Crossref record; 77 of the 77 references it cites were found in a registry or an index.
Developing a Cell Quenching Method to Facilitate Single Cell Mass Spectrometry Metabolomics Studies
Open accessPublished version of a preprint
Abstract
High Resolution Image Download MS PowerPoint Slide Single-cell mass spectrometry (SCMS) has emerged as a powerful tool for analyzing metabolites in individual cells, including live cells. However, cell metabolites have a rapid turnover rate, whereas maintaining metabolites’ profiles of live cells during sample transport, storage, or extended measurements can be challenging. In this study, a cell preparation method, which integrates cell washing by volatile salt solution, rapid liquid nitrogen (LN 2 ) quenching, freeze-drying in vacuum, and freezer storage at −80 °C, to preserve cell metabolites for SCMS measurement is discussed. Experimental results revealed that LN 2 quenching preserved the overall cell metabolome, whereas storage at −80 °C for 48 h slightly changed the metabolite profiles in quenched cells. However, metabolites in unquenched cells were changed regardless of low-temperature storage. The influence of omission of quenching and low-temperature storage on cell metabolites and relevant pathways were investigated. Results from this work indicate that cell quenching is necessary, but low-temperature storage time should be minimized to preserve cell metabolites. The method developed in the current work can be readily adopted by SCMS techniques with storage remaining largely unaltered, allowing for extended SCMS studies.
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Cites
HMDB 5.0: the Human Metabolome Database for 2022
Wishart, D. S., Guo, A., Oler, E., Wang, F., Anjum, A., Peters, H., Dizon, R., Sayeeda, Z., Tian, S., Lee, B. L., et al. (2022) · Nucleic Acids Research · doi:10.1093/nar/gkab1062 · Cited by 2,519
Pang, Z., Lu, Y., Zhou, G., Hui, F., Xu, L., Viau, C., Spigelman, A. F., MacDonald, P. E., Wishart, D. S., Li, S., et al. (2024) · Nucleic Acids Research · doi:10.1093/nar/gkae253 · Cited by 2,291
Gautier, J. (1993) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(93)90025-i · Cited by 2,165
Single-cell proteomic analysis of S. cerevisiae reveals the architecture of biological noise
Newman, J. R. S., Ghaemmaghami, S., Ihmels, J., Breslow, D. K., Noble, M., DeRisi, J. L., & Weissman, J. S. (2006) · Nature · doi:10.1038/nature04785 · Cited by 1,483
Single-cell genome sequencing: current state of the science
Gawad, C., Koh, W., & Quake, S. R. (2016) · Nature Reviews Genetics · doi:10.1038/nrg.2015.16 · Cited by 1,334
Mass Cytometry: Single Cells, Many Features
Spitzer, M. H., & Nolan, G. P. (2016) · Cell · doi:10.1016/j.cell.2016.04.019 · Cited by 1,199
Luby-Phelps, K. (1999) · International Review of Cytology · doi:10.1016/s0074-7696(08)60527-6 · Cited by 1,149
Mechanistic investigation of ionization suppression in electrospray ionization
King, R., Bonfiglio, R., Fernandez-Metzler, C., Miller-Stein, C., & Olah, T. (2000) · Journal of the American Society for Mass Spectrometry · doi:10.1016/s1044-0305(00)00163-x · Cited by 1,086
Milo, R. (2013) · BioEssays · doi:10.1002/bies.201300066 · Cited by 614
Mass Spectrometry: Recent Advances in Direct Open Air Surface Sampling/Ionization
Monge, M. E., Harris, G. A., Dwivedi, P., & Fernández, F. M. (2013) · Chemical Reviews · doi:10.1021/cr300309q · Cited by 476
Metadata
- Authors
- Wije Munige, S., Bhusal, D., Peng, Z., Chen, D., & Yang, Z.
- Affiliations
- Department of Chemistry and Biochemistry
- University of Oklahoma (US)
- OU Health Stephenson Cancer Center (US)
- Corresponding author
- Yang, Zhibo
- Venue
- JACS Au
- Publisher
- American Chemical Society (ACS)
- Published
- 2025-05-06
- Volume
- 5
- Issue
- 5
- Pages
- 2379-2387
- ISSN
- 2691-3704 (ISSN-L)
- PMC
- PMC12117403
- OpenAlex
- W4410122467
- Cited by
- 11
- References
- 77
- Citations by year
- 2026: 7 · 2025: 4
- FWCI
- 3.31
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc-nd
- creativecommons.org/licenses/by/4.0/ (vor)
- Locations
- JACS Au (publishedVersion, cc-by-nc-nd)
- ChemRxiv (acceptedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Division of Chemistry (2305182)
- National Institute of Allergy and Infectious Diseases (1R01AI177469)
- University of Oklahoma
- Chan Zuckerberg Initiative
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Keywords
- single-cell mass spectrometry, liquid nitrogen quenching, low-temperature storage, freeze-drying, sample preparation
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- The paper
- Wije Munige, S., Bhusal, D., Peng, Z., Chen, D., & Yang, Z. (2025). Developing a Cell Quenching Method to Facilitate Single Cell Mass Spectrometry Metabolomics Studies. JACS Au. https://doi.org/10.1021/jacsau.5c00327
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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