Cited by 123Advanced Proteomics Techniques and ApplicationsFood and Agricultural SciencesProteins in Food Systems

Comparison of different electrophoretic separations of hen egg white proteins

Desert, C., Guérin-Dubiard, C., Nau, F., Jan, G., Val, F., Mallard, J.·2001·Journal of Agricultural and Food Chemistry

Abstract

The hen egg white protein composition has not yet been fully defined. To improve the knowledge of this biological fluid, the most usual and recently developed electrophoretic methods have been used: SDS-PAGE, native-PAGE, isoelectric focusing (IEF), and 2-dimensional electrophoresis (2DE). Seven of the major known proteins were thus identified in at least one electrophoretic system. Isoforms of ovotransferrin, ovalbumin, and ovomucoid were visualized when pI was used for the separation. Two-dimensional electrophoresis allowed separation of a very large number of spots. In each of the four systems, some components were revealed but not identified, and unknown spots were particularly numerous with 2DE. With this technique, many spots corresponding to small acidic proteins were highlighted, among which was the Ch21 protein, whose presence in hen egg white was thus confirmed. This study thus constitutes, to our knowledge, the first proteomic investigation of hen egg white.

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Crossref · 10.1021/jf001423n

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Authors
Desert, C., Guérin-Dubiard, C., Nau, F., Jan, G., Val, F., & Mallard, J.
Affiliations
  • Ecole Nationale Supérieure Agronomique de Rennes and Research Laboratory for Dairy Technology (LRTL), Institut National de la Recherche Agronomique, 65 rue de Saint Brieuc, 35042 Rennes Cedex France
  • Institut National de la Recherche Agronomique (FR)
Venue
Journal of Agricultural and Food Chemistry
Published
2001-09-07
Volume
49
Issue
10
Pages
4553-4561
ISSN
  • 0021-8561 (ISSN-L)
  • 1520-5118
OpenAlex
W2058175369
Cited by
123
References
42
Citations by year
2026: 3 · 2025: 6 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 4 · 2020: 3 · 2019: 3 · 2018: 6 · 2017: 5 · 2016: 3 · 2015: 5 · 2014: 6 · 2013: 11 · 2012: 10
FWCI
1.51
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Agricultural and Food Chemistry (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Topics
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
Keywords
proteome, SDS-PAGE, native PAGE, isoelectric focusing, two-dimensional gel electrophoresis, ovomucoid, acidic proteins, protein identification
MeSH terms
Animals, Chickens, Conalbumin, Egg Proteins, Electrophoresis, Electrophoresis, Polyacrylamide Gel, Isoelectric Focusing, Isoelectric Point, Ovalbumin, Ovomucin, Blotting, Western

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