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Rapid HPLC Screening of Jasmonate-Induced Increases in Tobacco Alkaloids, Phenolics, and Diterpene Glycosides in Nicotiana attenuata
Abstract
A rapid, HPLC-based screening procedure for the main classes of secondary metabolites in Nicotiana attenuata leaves (alkaloids, phenolics, and diterpene glycosides) is reported. In a single step, leaves are extracted in aqueous acidified (0.5% acetic acid) methanol, and the extracted compounds are separated by reversed-phase HPLC with an acidic water/acetonitrile gradient in <30 min. The utility of the method in quantifying changes in the secondary metabolites after methyl jasmonate treatment of the plants, a treatment known to elicit resistance to herbivores in nature, is illustrated. Methyl jasmonate treatment elicited dramatic increases in some secondary metabolites (caffeoylputrescine, nicotine, and diterpene glycosides increased 12.5-, 1.4-, and 1.9-fold, respectively) but left others, such as rutin, unchanged. Such broad-based analytical screens will help characterize environmental and genetic changes in secondary metabolite profiles.
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Citations
Cited by
Light Regulation of Plant Defense
Ballaré, C. L. (2014) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-050213-040145 · Cited by 560
A functional genomics approach toward the understanding of secondary metabolism in plant cells
Goossens, A., Häkkinen, S. T., Laakso, I., Seppänen-Laakso, T., Biondi, S., De Sutter, V., Lammertyn, F., Nuutila, A. M., Söderlund, H., Zabeau, M., et al. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1032967100 · Cited by 406
Kessler, A., Halitschke, R., Diezel, C., & Baldwin, I. T. (2006) · Oecologia · doi:10.1007/s00442-006-0365-8 · Cited by 391
Self-citationHalitschke, R., & Baldwin, I. T. (2003) · The Plant Journal · doi:10.1046/j.1365-313x.2003.01921.x · Cited by 346
Self-citationJasmonate and salicylate induce expression of herbivore cytochrome P450 genes
Li, X., Schuler, M. A., & Berenbaum, M. R. (2002) · Nature · doi:10.1038/nature01003 · Cited by 263
Metadata
- Authors
- Keinänen, M., Oldham, N. J., & Baldwin, I. T.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2001-07-06
- Volume
- 49
- Issue
- 8
- Pages
- 3553-3558
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W1989971534
- Cited by
- 255
- References
- 0
- FWCI
- 3.26
- Type
- journal article
- Language
- en
- Open access
- closed
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- Keinänen, M., Oldham, N. J., & Baldwin, I. T. (2001). Rapid HPLC Screening of Jasmonate-Induced Increases in Tobacco Alkaloids, Phenolics, and Diterpene Glycosides in Nicotiana attenuata. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/jf010200%2B
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