Isolation and Purification of Lutein from the Microalga Chlorella vulgaris by Extraction after Saponification
Abstract
A simple and efficient method for the isolation and purification of lutein from the microalga Chlorella vulgaris was developed. Crude lutein was obtained by extraction with dichloromethane from the microalga after saponification. Partition values of lutein in the two-phase system of ethanol-water-dichloromethane at different ratios were measured by HPLC so as to assist the determination of an appropriate condition for washing water-soluble impurities in the crude lutein. Partition values of lutein in another two-phase system of ethanol-water-hexane at different ratios were also measured by HPLC for determining the condition for removing fat-soluble impurities. The water-soluble impurities in the crude lutein were removed by washing with 30% aqueous ethanol, and the fat-soluble impurities were removed by extraction with hexane. The final purity of lutein obtained was 90-98%, and the yield was 85-91%.
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Chlorella vulgaris is sold as a food supplement, additive, food color, and emulsion for food products.
“Chlorella vulgaris is sold as a food supplement, an additive [117,118], a food color, and an emulsion for food products [119].”
From Challenges in Functional Food Products with the Incorporation of Some Microalgae (2024) · cites this paper as [117]
Cites
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High-value products from microalgae—their development and commercialisation
Borowitzka, M. A. (2013) · Journal of Applied Phycology · doi:10.1007/s10811-013-9983-9 · Cited by 1,585 · see citing works
‘Morphology, composition, production, processing and applications of Chlorella vulgaris: a review’.
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Self-citationIndustrial potential of carotenoid pigments from microalgae: Current trends and future prospects
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Challenges in Functional Food Products with the Incorporation of Some Microalgae
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- Authors
- Li, H.-B., Jiang, Y., & Chen, F.
- Affiliations
- University of Hong Kong (HK)
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2002-01-17
- Volume
- 50
- Issue
- 5
- Pages
- 1070-1072
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2077270544
- Cited by
- 74
- References
- 2
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 5 · 2023: 5 · 2022: 5 · 2021: 5 · 2020: 5 · 2019: 6 · 2018: 3 · 2017: 2 · 2016: 3 · 2015: 1 · 2014: 2 · 2013: 5 · 2012: 1
- FWCI
- 1.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- The HKU Scholars Hub (University of Hong Kong) (submittedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
- Keywords
- lutein, Chlorella vulgaris, microalgae, saponification, extraction, purification, carotenoids
- MeSH terms
- Chlorella, Chromatography, High Pressure Liquid, Indicators and Reagents, Lutein, Reproducibility of Results
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