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Total Cranberry Extract versus Its Phytochemical Constituents: Antiproliferative and Synergistic Effects against Human Tumor Cell Lines
Abstract
Cranberries (Vaccinium macrocarpon Ait.) are an excellent dietary source of phytochemicals that include flavonol glycosides, anthocyanins, proanthocyanidins (condensed tannins), and organic and phenolic acids. Using C-18 and Sephadex Lipophilic LH-20 column chromatography, HPLC, and tandem LC-ES/MS, the total cranberry extract (TCE) has been analyzed, quantified, and separated into fractions enriched in sugars, organic acids, total polyphenols, proanthocyanidins, and anthocyanins (39.4, 30.0, 10.6, 5.5, and 1.2% composition, respectively). Using a luminescent ATP cell viability assay, the antiproliferative effects of TCE (200 microg/mL) versus all fractions were evaluated against human oral (KB, CAL27), colon (HT-29, HCT116, SW480, SW620), and prostate (RWPE-1, RWPE-2, 22Rv1) cancer cell lines. The total polyphenol fraction was the most active fraction against all cell lines with 96.1 and 95% inhibition of KB and CAL27 oral cancer cells, respectively. For the colon cancer cells, the antiproliferative activity of this fraction was greater against HCT116 (92.1%) than against HT-29 (61.1%), SW480 (60%), and SW620 (63%). TCE and all fractions showed >/=50% antiproliferative activity against prostate cancer cells with total polyphenols being the most active fraction (RWPE-1, 95%; RWPE-2, 95%; 22Rv1, 99.6%). Cranberry sugars (78.8 microg/mL) did not inhibit the proliferation of any cancer cell lines. The enhanced antiproliferative activity of total polyphenols compared to TCE and its individual phytochemicals suggests synergistic or additive antiproliferative interactions of the anthocyanins, proanthocyanidins, and flavonol glycosides within the cranberry extract.
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Citations
Cited by
Anthocyanins: Natural Colorants with Health-Promoting Properties
He, J., & Giusti, M. M. (2010) · Annual Review of Food Science and Technology · doi:10.1146/annurev.food.080708.100754 · Cited by 1,637
Butelli, E., Titta, L., Giorgio, M., Mock, H.-P., Matros, A., Peterek, S., Schijlen, E. G. W. M., Hall, R. D., Bovy, A. G., Luo, J., et al. (2008) · Nature Biotechnology · doi:10.1038/nbt.1506 · Cited by 1,238
Seeram, N. P., Adams, L. S., Henning, S. M., Niu, Y., Zhang, Y., Nair, M. G., & Heber, D. (2005) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2005.01.006 · Cited by 1,112
Self-citationSeeram, N. P., Adams, L. S., Zhang, Y., Lee, R., Sand, D., Scheuller, H. S., & Heber, D. (2006) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf061750g · Cited by 770
Fruit Development and Ripening
Seymour, G. B., Østergaard, L., Chapman, N. H., Knapp, S., & Martin, C. (2013) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-050312-120057 · Cited by 658
Metadata
- Authors
- Seeram, N. P., Adams, L. S., Hardy, M. L., & Heber, D.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2004-04-07
- Volume
- 52
- Issue
- 9
- Pages
- 2512-2517
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2166744136
- Cited by
- 404
- References
- 0
- FWCI
- 7.87
- Type
- journal article
- Language
- en
- Open access
- closed
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- Seeram, N. P., Adams, L. S., Hardy, M. L., & Heber, D. (2004). Total Cranberry Extract versus Its Phytochemical Constituents: Antiproliferative and Synergistic Effects against Human Tumor Cell Lines. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/jf0352778
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