Cited by 772Phytochemicals and Antioxidant ActivitiesPomegranate: compositions and health benefitsBerry genetics and cultivation research

Blackberry, Black Raspberry, Blueberry, Cranberry, Red Raspberry, and Strawberry Extracts Inhibit Growth and Stimulate Apoptosis of Human Cancer Cells in Vitro

Seeram, Navindra P., Adams, Lynn S., Zhang, Yanjun, Lee, Rupo, Sand, Daniel, Scheuller, Henry S., Heber, David·2006·Journal of Agricultural and Food Chemistry

Abstract

Berry fruits are widely consumed in our diet and have attracted much attention due to their potential human health benefits. Berries contain a diverse range of phytochemicals with biological properties such as antioxidant, anticancer, anti-neurodegerative, and anti-inflammatory activities. In the current study, extracts of six popularly consumed berries--blackberry, black raspberry, blueberry, cranberry, red raspberry and strawberry--were evaluated for their phenolic constituents using high performance liquid chromatography with ultraviolet (HPLC-UV) and electrospray ionization mass spectrometry (LC-ESI-MS) detection. The major classes of berry phenolics were anthocyanins, flavonols, flavanols, ellagitannins, gallotannins, proanthocyanidins, and phenolic acids. The berry extracts were evaluated for their ability to inhibit the growth of human oral (KB, CAL-27), breast (MCF-7), colon (HT-29, HCT116), and prostate (LNCaP) tumor cell lines at concentrations ranging from 25 to 200 micro g/mL. With increasing concentration of berry extract, increasing inhibition of cell proliferation in all of the cell lines were observed, with different degrees of potency between cell lines. The berry extracts were also evaluated for their ability to stimulate apoptosis of the COX-2 expressing colon cancer cell line, HT-29. Black raspberry and strawberry extracts showed the most significant pro-apoptotic effects against this cell line. The data provided by the current study and from other laboratories warrants further investigation into the chemopreventive and chemotherapeutic effects of berries using in vivo models.

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Crossref · 10.1021/jf061750g

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Authors
Seeram, N. P., Adams, L. S., Zhang, Y., Lee, R., Sand, D., Scheuller, H. S., & Heber, D.
Affiliations
  • University of California, Los Angeles (US)
  • Center for Human Nutrition, David Geffen School of Medicine, University of California, Los Angeles, California 90095
Venue
Journal of Agricultural and Food Chemistry
Published
2006-11-18
Volume
54
Issue
25
Pages
9329-9339
ISSN
  • 0021-8561 (ISSN-L)
  • 1520-5118
OpenAlex
W2091770324
Cited by
770
References
23
Citations by year
2026: 12 · 2025: 19 · 2024: 29 · 2023: 32 · 2022: 27 · 2021: 42 · 2020: 48 · 2019: 41 · 2018: 43 · 2017: 42 · 2016: 50 · 2015: 52 · 2014: 52 · 2013: 56 · 2012: 68
FWCI
17.71
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Agricultural and Food Chemistry (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Cancer Institute (P01 CA 42710, P01CA042710)
Topics
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
  • Health Sciences › Nursing › Nutrition and Dietetics › Pomegranate: compositions and health benefits
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Berry genetics and cultivation research
Keywords
berry extracts, blackberry, black raspberry, blueberry, cranberry, red raspberry, strawberry, phenolic compounds, anthocyanins, proanthocyanidins, ellagitannins, flavonoids, phenolic acids, anticancer activity, apoptosis, cell proliferation inhibition, colon cancer, breast cancer, oral cancer, prostate cancer, cyclooxygenase-2, chemoprevention
MeSH terms
Cell Division, Chromatography, High Pressure Liquid, Fruit, Humans, Neoplasms, Phenols, Plant Extracts, Apoptosis, Rosales, Spectrometry, Mass, Electrospray Ionization, Rosaceae, Vaccinium, Blueberry Plants, Vaccinium macrocarpon, Fragaria, Cell Line, Tumor

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