Blackberry, Black Raspberry, Blueberry, Cranberry, Red Raspberry, and Strawberry Extracts Inhibit Growth and Stimulate Apoptosis of Human Cancer Cells in Vitro
Abstract
Berry fruits are widely consumed in our diet and have attracted much attention due to their potential human health benefits. Berries contain a diverse range of phytochemicals with biological properties such as antioxidant, anticancer, anti-neurodegerative, and anti-inflammatory activities. In the current study, extracts of six popularly consumed berries--blackberry, black raspberry, blueberry, cranberry, red raspberry and strawberry--were evaluated for their phenolic constituents using high performance liquid chromatography with ultraviolet (HPLC-UV) and electrospray ionization mass spectrometry (LC-ESI-MS) detection. The major classes of berry phenolics were anthocyanins, flavonols, flavanols, ellagitannins, gallotannins, proanthocyanidins, and phenolic acids. The berry extracts were evaluated for their ability to inhibit the growth of human oral (KB, CAL-27), breast (MCF-7), colon (HT-29, HCT116), and prostate (LNCaP) tumor cell lines at concentrations ranging from 25 to 200 micro g/mL. With increasing concentration of berry extract, increasing inhibition of cell proliferation in all of the cell lines were observed, with different degrees of potency between cell lines. The berry extracts were also evaluated for their ability to stimulate apoptosis of the COX-2 expressing colon cancer cell line, HT-29. Black raspberry and strawberry extracts showed the most significant pro-apoptotic effects against this cell line. The data provided by the current study and from other laboratories warrants further investigation into the chemopreventive and chemotherapeutic effects of berries using in vivo models.
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Isolated polyphenols (anthocyanins, kaempferol, and quercetin) inhibit the growth of various human tumor cell lines in a dose-dependent manner, with varying sensitivities.
“For example, isolated polyphenols (anthocyanins, kaempferol, and quercetin) inhibit the growth of various human tumor cell lines in a dose-dependent manner, with varying sensitivities [68,69].”
From Phytoecdysteroids: Quantification in Selected Plant Species and Evaluation of Some Effects on Gastric Smooth Muscles (2024) · cites this paper as [69]
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- Authors
- Seeram, N. P., Adams, L. S., Zhang, Y., Lee, R., Sand, D., Scheuller, H. S., & Heber, D.
- Affiliations
- University of California, Los Angeles (US)
- Center for Human Nutrition, David Geffen School of Medicine, University of California, Los Angeles, California 90095
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2006-11-18
- Volume
- 54
- Issue
- 25
- Pages
- 9329-9339
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2091770324
- Cited by
- 770
- References
- 23
- Citations by year
- 2026: 12 · 2025: 19 · 2024: 29 · 2023: 32 · 2022: 27 · 2021: 42 · 2020: 48 · 2019: 41 · 2018: 43 · 2017: 42 · 2016: 50 · 2015: 52 · 2014: 52 · 2013: 56 · 2012: 68
- FWCI
- 17.71
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute (P01 CA 42710, P01CA042710)
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Health Sciences › Nursing › Nutrition and Dietetics › Pomegranate: compositions and health benefits
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Berry genetics and cultivation research
- Keywords
- berry extracts, blackberry, black raspberry, blueberry, cranberry, red raspberry, strawberry, phenolic compounds, anthocyanins, proanthocyanidins, ellagitannins, flavonoids, phenolic acids, anticancer activity, apoptosis, cell proliferation inhibition, colon cancer, breast cancer, oral cancer, prostate cancer, cyclooxygenase-2, chemoprevention
- MeSH terms
- Cell Division, Chromatography, High Pressure Liquid, Fruit, Humans, Neoplasms, Phenols, Plant Extracts, Apoptosis, Rosales, Spectrometry, Mass, Electrospray Ionization, Rosaceae, Vaccinium, Blueberry Plants, Vaccinium macrocarpon, Fragaria, Cell Line, Tumor
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