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Production of Prebiotic Galacto-Oligosaccharides from Lactose Using β-Galactosidases from Lactobacillus reuteri
Abstract
The beta-galactosidases (beta-Gals) of Lactobacillus reuteri L103 and L461 proved to be suitable biocatalysts for the production of prebiotic galacto-oligosaccharides (GOS) from lactose. Maximum GOS yields were 38% when using an initial lactose concentration of 205 g/L and at approximately 80% lactose conversion. The product mixtures were analyzed by capillary electrophoresis (CE) and high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). Disaccharides other than lactose and trisaccharides made up the vast majority of GOS formed. The main products were identified as beta-d-Galp-(1-->6)-d-Glc (allolactose), beta-d-Galp-(1-->6)-d-Gal, beta-d-Galp-(1-->3)-d-Gal, beta-d-Galp-(1-->6)-Lac, and beta-d-Galp-(1-->3)-Lac. There were no major products with beta1-->4 linkages formed. Both intermolecular and intramolecular transgalactosylation were observed. d-Galactose proved to be a very efficient galactosyl acceptor; thus, a relatively large amount of galactobioses was formed. Monosaccharides could be conveniently separated from the mixture by chromatography using a strong cation-exchange resin.
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Cited by
LoCascio, R. G., Ninonuevo, M. R., Freeman, S. L., Sela, D. A., Grimm, R., Lebrilla, C. B., Mills, D. A., & German, J. B. (2007) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf0710480 · Cited by 365
Recent advances refining galactooligosaccharide production from lactose
Gosling, A., Stevens, G. W., Barber, A. R., Kentish, S. E., & Gras, S. L. (2010) · Food Chemistry · doi:10.1016/j.foodchem.2009.12.063 · Cited by 313
Synthesis of Lactose-Derived Nutraceuticals from Dairy Waste Whey—a Review
Nath, A., Verasztó, B., Basak, S., Koris, A., Kovács, Z., & Vatai, G. (2016) · Food and Bioprocess Technology · doi:10.1007/s11947-015-1572-2 · Cited by 71
Metadata
- Authors
- Splechtna, B., Nguyen, T.-H., Steinböck, M., Kulbe, K. D., Lorenz, W., & Haltrich, D.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2006-06-21
- Volume
- 54
- Issue
- 14
- Pages
- 4999-5006
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W1986017237
- Cited by
- 204
- References
- 0
- FWCI
- 8.69
- Type
- journal article
- Language
- en
- Open access
- closed
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- Splechtna, B., Nguyen, T.-H., Steinböck, M., Kulbe, K. D., Lorenz, W., & Haltrich, D. (2006). Production of Prebiotic Galacto-Oligosaccharides from Lactose Using β-Galactosidases from Lactobacillus reuteri. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/jf053127m
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