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Comparative Evaluation of Three Lignin Isolation Protocols for Various Wood Species
Abstract
Milled wood lignin (MWL), cellulolytic enzyme lignin (CEL), and enzymatic mild acidolysis lignin (EMAL) were isolated from different wood species and characterized by various techniques. The EMAL protocol offered gravimetric lignin yields 2-5 times greater than those of the corresponding MWL and CEL. The purities of the EMALs were 3.75-10.6% higher than those of their corresponding CELs, depending upon the wood species from which they were isolated. Molecular weight analyses showed that the EMAL protocol isolates lignin fractions that are not accessed by the other procedures evaluated, while 31P NMR spectroscopy revealed that MWL is more condensed and bears more phenolic hydroxyl groups than EMAL and CEL. The yields and purities of EMAL, MWL, and CEL from hardwood were greater than those obtained for the examined softwoods. Structural details obtained by DFRC (derivatization followed by reductive cleavage)/31P NMR revealed different contents of condensed and uncondensed beta-O-aryl ether structures, dibenzodioxocins, and condensed and uncondensed phenolic hydroxyl and carboxylic acid groups within lignins isolated from different wood species.
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Cited by
Catalytic Transformation of Lignin for the Production of Chemicals and Fuels
Li, C., Zhao, X., Wang, A., Huber, G. W., & Zhang, T. (2015) · Chemical Reviews · doi:10.1021/acs.chemrev.5b00155 · Cited by 2,951
A review on lignin-based polymeric, micro- and nano-structured materials
Duval, A., & Lawoko, M. (2014) · Reactive and Functional Polymers · doi:10.1016/j.reactfunctpolym.2014.09.017 · Cited by 733
Characterization and analysis of the molecular weight of lignin for biorefining studies
Tolbert, A., Akinosho, H., Khunsupat, R., Naskar, A. K., & Ragauskas, A. J. (2014) · Biofuels, Bioproducts and Biorefining · doi:10.1002/bbb.1500 · Cited by 504
Oxidative upgrade of lignin – Recent routes reviewed
Lange, H., Decina, S., & Crestini, C. (2013) · European Polymer Journal · doi:10.1016/j.eurpolymj.2013.03.002 · Cited by 473
Yu, H., Liu, F., Ke, M., & Zhang, X. (2015) · Bioresource Technology · doi:10.1016/j.biortech.2015.03.005 · Cited by 38
One-pot quantitative hydrolysis of lignocelluloses mediated by black liquor
Zhu, Z., Sun, M. M., Su, C., Zhao, H., Ma, X., Zhu, Z., Shi, X., & Gu, K. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2012.10.072 · Cited by 19
Metadata
- Authors
- Guerra, A., Filpponen, I., Lucia, L. A., & Argyropoulos, D. S.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2006-12-01
- Volume
- 54
- Issue
- 26
- Pages
- 9696-9705
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2087882438
- Cited by
- 253
- References
- 0
- FWCI
- 5.36
- Type
- journal article
- Language
- en
- Open access
- closed
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