Isolation and Identification of Strawberry Phenolics with Antioxidant and Human Cancer Cell Antiproliferative Properties
Abstract
Studies suggest that consumption of berry fruits, including strawberries ( Fragaria x ananassa Duch.), may have beneficial effects against oxidative stress mediated diseases such as cancer. Berries contain multiple phenolic compounds, which are thought to contribute to their biological properties. Comprehensive profiling of phenolics from strawberries was previously reported using high-performance liquid chromatography with mass spectrometry (HPLC-MS) detection. The current study reports the isolation and structural characterization of 10 phenolic compounds from strawberry extracts using a combination of Amberlite XAD16-resin and C18 columns, HPLC-UV, and nuclear magnetic resonance (NMR) spectroscopy methods. The phenolics were cyanidin-3-glucoside ( 1), pelargonidin (2), pelargonidin-3-glucoside (3), pelargonidin-3-rutinoside (4), kaempferol (5), quercetin (6), kaempferol-3-(6'-coumaroyl)glucoside) (7), 3,4,5-trihydroxyphenyl-acrylic acid (8), glucose ester of ( E)- p-coumaric acid (9), and ellagic acid . Strawberry crude extracts and purified compounds 1- 10 were evaluated for antioxidant and human cancer cell antiproliferative activities by the Trolox equivalent antioxidant capacity (TEAC) and luminescent ATP cell viability assays, respectively. Among the pure compounds, the anthocyanins 1 (7156 microM Trolox/mg), 2 (4922 microM Trolox/mg), and 4 (5514 microM Trolox/mg) were the most potent antioxidants. Crude extracts (250 microg/mL) and pure compounds (100 microg/mL) inhibited the growth of human oral (CAL-27, KB), colon (HT29, HCT-116), and prostate (LNCaP, DU145) cancer cells with different sensitivities observed between cell lines. This study adds to the growing body of data supporting the bioactivities of berry fruit phenolics and their potential impact on human health.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Isolated polyphenols (anthocyanins, kaempferol, and quercetin) inhibit the growth of various human tumor cell lines in a dose-dependent manner, with varying sensitivities.
“For example, isolated polyphenols (anthocyanins, kaempferol, and quercetin) inhibit the growth of various human tumor cell lines in a dose-dependent manner, with varying sensitivities [68,69].”
From Phytoecdysteroids: Quantification in Selected Plant Species and Evaluation of Some Effects on Gastric Smooth Muscles (2024) · cites this paper as [68]
Cited by
Sorted by citations
Plant Phenolics: Extraction, Analysis and Their Antioxidant and Anticancer Properties
Dai, J., & Mumper, R. J. (2010) · Molecules · doi:10.3390/molecules15107313 · Cited by 4,541 · see citing works
“acid, were shown to inhibit the growth of human oral (KB, CAL-27), breast (MCF-7), colon (HT-29, HCT-116), and prostate (LNCaP, DU-145) tumor cell lines in a dose-dependent manner with different sensitivity between cell lines [66,159].”
InfluentialAnthocyanins: Natural Colorants with Health-Promoting Properties
He, J., & Giusti, M. M. (2010) · Annual Review of Food Science and Technology · doi:10.1146/annurev.food.080708.100754 · Cited by 1,652 · see citing works
The strawberry: Composition, nutritional quality, and impact on human health
Giampieri, F., Tulipani, S., Álvarez-Suárez, J. M., Quiles, J. L., Mezzetti, B., & Battino, M. A. (2011) · Nutrition · doi:10.1016/j.nut.2011.08.009 · Cited by 1,051 · see citing works
Anthocyanins and their role in cancer prevention
Wang, L., & Stoner, G. D. (2008) · Cancer Letters · doi:10.1016/j.canlet.2008.05.020 · Cited by 1,045 · see citing works
“Anti-cell proliferation—Pure anthocyanins and anthocyanin-rich extracts from fruits and vegetables have exhibited anti-proliferative activity towards multiple cancer cell types in vitro [17–22].”
BackgroundBioactive Compounds and Antioxidant Activity in Different Types of Berries
Škrovánková, S., Sumczynski, D., Mlček, J., Juríková, T., & Sochor, J. (2015) · International Journal of Molecular Sciences · doi:10.3390/ijms161024673 · Cited by 1,009 · see citing works
“Thus, they could suppress the growth of human oral, colon, and prostate cancer cells [59,60].”
BackgroundPhenolics: From Chemistry to Biology
Pereira, D. M., Valentão, P., Pereira, J. A., & Andrade, P. B. (2009) · Molecules · doi:10.3390/molecules14062202 · Cited by 709 · see citing works
“Recently, significant anti-cancer properties of some anthocyanins against a range of cell lines have been described [35].”
BackgroundThe Case for Anthocyanin Consumption to Promote Human Health: A Review
Pojer, E., Mattivi, F., Johnson, D., & Stockley, C. S. (2013) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.12024 · Cited by 636 · see citing works
Flavonoids and phenolic acids: Role and biochemical activity in plants and human
Ghasemzadeh, A. (2011) · Journal of Medicinal Plants Research · doi:10.5897/jmpr11.1404 · Cited by 656 · see citing works
“…polyphenols from strawberry including kaempferol, quercetin, anthocyanins, coumaric acid and ellagic acid, were shown to inhibit the growth of human breast (MCF7), oral (KB, CAL-27), colon (HT-29, HCT-116), and prostate (LNCaP, DU-145) tumor cell lines (Zhang et al., 2008; Damianaki et al., 2000).”
BackgroundChemistry, Pharmacology and Health Benefits of Anthocyanins
Smeriglio, A., Barreca, D., Bellocco, E., & Trombetta, D. (2016) · Phytotherapy Research · doi:10.1002/ptr.5642 · Cited by 449 · see citing works
Plant derived and dietary phenolic antioxidants: Anticancer properties
Roleira, F. M. F., da Silva, E. J. T., Varela, C. L., Costa, S. C., Silva, T., Garrido, J. M. P. J., & Borges, F. (2015) · Food Chemistry · doi:10.1016/j.foodchem.2015.03.039 · Cited by 431 · see citing works
“…(Kawai et al., 2008) ANTHOCYANINS AND ANTHOCYANIDINS Pelargonidin Strawberry KB HT-29, HCT116 CAL-27 LNCaP, DU145 Cervical Colon Oral cavity Prostate (Zhang et al., 2008) Pelargonidin-3-glucoside Strawberry KB HT-29, HCT116 CAL-27 LNCaP, DU145 Cervical Colon Oral cavity Prostate (Zhang et al.,…”
BackgroundInfluentialStrawberry as a health promoter: an evidence based review
Giampieri, F., Forbes-Hernández, T. Y., Gasparrini, M., Álvarez-Suárez, J. M., Afrin, S., Bompadre, S., Quiles, J. L., Mezzetti, B., & Battino, M. A. (2015) · Food & Function · doi:10.1039/c5fo00147a · Cited by 386 · see citing works
“Anticarcinogenic e ff ects of strawberries are mediated mainly through the detoxification of carcinogens, scavenging of reactive oxygen species, the decrease of oxidative DNA damage, 49,50 the reduction of cancer cell proliferation through apoptosis 51 and cell-cycle arrest, 48 downregulation of the activator protein-1…”
BackgroundInfluentialMahato, N., Sinha, M., Sharma, K., Koteswararao, R., & Cho, M. H. (2019) · Foods · doi:10.3390/foods8110523 · Cited by 361 · see citing works
“, n-Hexane [197]; dichloromethane [198], or chloroform [199].”
Strawberry As a Functional Food: An Evidence-Based Review
Basu, A., Nguyen, A., Betts, N. M., & Lyons, T. J. (2014) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2011.608174 · Cited by 312 · see citing works
“Purified ellagic acid from strawberries has been shown to contribute to the anticarcinogenic effects of strawberries in several human cancer cell models (Zhang et al., 2008; Sharma et al., 2010).”
BackgroundPromising Health Benefits of the Strawberry: A Focus on Clinical Studies
Afrin, S., Gasparrini, M., Forbes-Hernández, T. Y., Reboredo‐Rodríguez, P., Mezzetti, B., Varela‐López, A., Giampieri, F., & Battino, M. A. (2016) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.6b00857 · Cited by 309 · see citing works
Human cancer cell antiproliferative and antioxidant activities of Juglans regia L.
Carvalho, M. C., Ferreira, P. J., Mendes, V., Silva, R., Pereira, J. A., Jerónimo, C., & Silva, B. M. (2009) · Food and Chemical Toxicology · doi:10.1016/j.fct.2009.10.043 · Cited by 299 · see citing works
“…conducted on cell cultures and animal models seem to indicate that polyphenols are the main phytochemicals with antioxidant and antiproliferative properties of higher plants (Silva et al., 2004, 2008; Giada and Filho, 2006; Fiorentino et al., 2008; Zhang et al., 2008; Mertens-Talcott et al., 2006).”
BackgroundDhalaria, R., Verma, R., Kumar, D., Puri, S., Tapwal, A., Kumar, V., Nepovimová, E., & Kuča, K. (2020) · Antioxidants · doi:10.3390/antiox9111123 · Cited by 262 · see citing works
Natural and Synthetic Flavylium-Based Dyes: The Chemistry Behind the Color
Cruz, L., Basílio, N., Mateus, N. F. D. C. B., de Freitas, V. A. P., & Piña, F. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.1c00399 · Cited by 260 · see citing works
Antioxidant and anticancer properties of berries
Baby, B., Antony, P., & Vijayan, R. (2017) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2017.1329198 · Cited by 227 · see citing works
“Furthermore, an in vitro study by Zhang and colleagues demonstrated the anti-proliferative effect of strawberry crude extracts with ellagic acid on human colon (HT-29 and HCT116), oral (KB and CAL-27), and prostate (LNCaP and DU145) cell lines in a dose-dependent manner (Zhang et al., 2008).”
BackgroundReview of Functional and Pharmacological Activities of Berries
Головинская, О., & Wang, C. (2021) · Molecules · doi:10.3390/molecules26133904 · Cited by 232 · see citing works
“Moreover, an in vitro study showed anti-proliferative effect of strawberry extracts with ellagic acid on the human colon, prostate, and oral cell lines [139].”
BackgroundPolyphenols in Fruits and Vegetables and Its Effect on Human Health
Lima, G. P. P., Vianello, F., Corrêa, C. R., Campos, R. A. D. S., & Borguini, M. G. (2014) · Food and Nutrition Sciences · doi:10.4236/fns.2014.511117 · Cited by 215 · see citing works
“This fruit is consumed in fresh form, in processed food products, and as extracts for dietary supplements [108].”
BackgroundThe Strawberry Plant Defense Mechanism: A Molecular Review
Amil‐Ruíz, F., Blanco‐Portales, R., Muñoz‐Blanco, J., & Caballero, J. L. (2011) · Plant and Cell Physiology · doi:10.1093/pcp/pcr136 · Cited by 216 · see citing works
Diaconeasa, Z. M., LEOPOLD, L. F., Rugină, D. O., Ayvaz, H., & Socaciu, C. (2015) · International Journal of Molecular Sciences · doi:10.3390/ijms16022352 · Cited by 214 · see citing works
“studies that reported high antiproliferative activity of ARFs from different berries [18,34,55,56].”
Choi, H. G., Moon, B. Y., & Kang, N. J. (2015) · Scientia Horticulturae · doi:10.1016/j.scienta.2015.03.022 · Cited by 201 · see citing works
“The functional ingredients of strawberry fruits are represented by many secondary metabolites, such as anthocyanins, flavonoids, and phenolic compounds, which are presumed to be used defensively to prevent harmful damage caused by reactive oxygen species (Gil et al., 1997; Zhang et al., 2008; Zheng et al., 2007).”
BackgroundFarias, D. D. P., Neri-Numa, I. A., de Araújo, F. F., & Pastore, G. M. (2019) · Food Chemistry · doi:10.1016/j.foodchem.2019.125630 · Cited by 196 · see citing works
Health promoting properties of blueberries: a review
Silva, S., Costa, E. M., Veiga, M., Morais, R. M. S. C., Calhau, C., & Pintado, M. (2018) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2018.1518895 · Cited by 188 · see citing works
“…nitric oxide synthase (iNOS), of COX-2 expression which is frequently upregulated in CVD and inhibition of the NF-jB pathway) may also play an important role in reducing the risk of developing these pathologies (Danesh et al. 2000; Riso et al. 2013; Basu, Rhone, and Lyons 2010; Zhang et al. 2008).”
BackgroundMa, H., Johnson, S. L., Liu, W., DaSilva, N. A., Meschwitz, S. M., Dain, J. A., & Seeram, N. P. (2018) · International Journal of Molecular Sciences · doi:10.3390/ijms19020461 · Cited by 181 · see citing works
Self-citationJimenez-Garcia, S. N., Guevara-González, R. G., Miranda‐López, R., Feregrino‐Pérez, A. A., Torres‐Pacheco, I., & Vazquez-Cruz, M. A. (2012) · Food Research International · doi:10.1016/j.foodres.2012.11.004 · Cited by 171 · see citing works
Pro-Oxidant Natural Products as Anticancer Agents
Martı́n-Cordero, C., León‐González, A. J., Calderón‐Montaño, J. M., Burgos‐Morón, E., & López‐Lázaro, M. (2012) · Current Drug Targets · doi:10.2174/138945012802009044 · Cited by 171 · see citing works
Chitosan biopolymer promotes yield and stimulates accumulation of antioxidants in strawberry fruit
Rahman, M., Rahman, M., Mukta, J. A., et al. (2018) · PLoS ONE · doi:10.1371/journal.pone.0203769 · Cited by 171 · see citing works
“significant anticancer [2, 3, 6] and antioxidant [7] activities.”
Extraction, characterization and biological activity of citrus flavonoids
Sharma, K., Mahato, N., & Lee, Y. R. (2018) · Reviews in Chemical Engineering · doi:10.1515/revce-2017-0027 · Cited by 160 · see citing works
Afrin, S., Giampieri, F., Gasparrini, M., Forbes-Hernández, T. Y., Varela‐López, A., Quiles, J. L., Mezzetti, B., & Battino, M. A. (2016) · Molecules · doi:10.3390/molecules21020169 · Cited by 159 · see citing works
Mahmood, T., Anwar, F., Abbas, M., & Saari, N. (2012) · International Journal of Molecular Sciences · doi:10.3390/ijms13044591 · Cited by 157 · see citing works
“Previous studies reported methanol soluble cinnamic acid and p-hydroxycinnamic acid in strawberries to be the major components followed by caffeic acid and ferulic acid [2,51].”
BackgroundYang, B., Ahotupa, M. O., Määttä, P., & Kallio, H. P. T. (2011) · Food Research International · doi:10.1016/j.foodres.2011.02.025 · Cited by 150 · see citing works
Sharma, M., Li, L., Celver, J. P., Killian, C., Kovoor, A., & Seeram, N. P. (2009) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf902857v · Cited by 150 · see citing works
“Although all these fruits contain anthocyanins, we followed protocols that enriched the extracts in ET-content, while removing anthocyanins, as previously described for pomegranate peel (17) and strawberry fruit (18).”
BackgroundMethodSelf-citationComparison of antioxidant capacity and phenolic compounds of berries, chokecherry and seabuckthorn
Li, W., Hydamaka, A., Lowry, L., & Trust Beta (2009) · Open Life Sciences · doi:10.2478/s11535-009-0041-1 · Cited by 148 · see citing works
“Anticancer effects of berry fruit bioactives have been confirmed, such as strawberry extracts inhibiting the growth of human oral, colon and prostate cancer cells [10], cranberry proanthocyanidin extract inhibiting the viability and proliferation of human esophageal adenocarcinoma cells [14].”
BackgroundKadomura-Ishikawa, Y., Miyawaki, K., Noji, S., & Takahashi, A. (2013) · Journal of Plant Research · doi:10.1007/s10265-013-0582-2 · Cited by 143 · see citing works
Xu, F., Shi, L., Chen, W., Cao, S., Su, X., & Yang, Z. (2014) · Scientia Horticulturae · doi:10.1016/j.scienta.2014.06.012 · Cited by 143 · see citing works
Li, L., Adams, L. S., Chen, S., Killian, C., Ahmed, A., & Seeram, N. P. (2009) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf803407q · Cited by 138 · see citing works
“The Jamun fruit extract was enriched in anthocyanins and prepared according to previously published methods with slight modifications (8,9).”
MethodSelf-citationAntiproliferative activity of peels, pulps and seeds of 61 fruits
Li, F., Li, S., Li, H., Deng, G., Ling, W., Wu, S., Xu, X., & Chen, F. (2013) · Journal of Functional Foods · doi:10.1016/j.jff.2013.04.016 · Cited by 134 · see citing works
Garcia-Muñoz, C., & Vaillant, F. (2014) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2011.644643 · Cited by 133 · see citing works
“Consequently, as shown in Table 1, potential chemopreventive benefits of dietary ETs and EA have been studied mostly for cancer cell lines from colon (Narayanan and Re, 2001; Losso et al., 2004; Seeram et al., 2005; Seeram et al., 2006a; Larrosa et al., 2006b; Zhang et al., 2008; GonzalezSarrias et al., 2009a; Gonzale…”
BackgroundInfluentialNovel botanical ingredients for beverages
Gruenwald, J. (2009) · Clinics in Dermatology · doi:10.1016/j.clindermatol.2008.11.003 · Cited by 131 · see citing works
Isolation, identification, and antioxidant activity of anthocyanin compounds in Camarosa strawberry
Cerezo, A. B., Montilla, E. C., Winterhalter, P., García-Parrilla, M. C., & Troncoso, A. M. (2010) · Food Chemistry · doi:10.1016/j.foodchem.2010.04.073 · Cited by 127 · see citing works
Antioxidative and in vitro antiproliferative activity of Arctium lappa root extracts
Predes, F. D. S., Ruiz, A. L. T. G., de Carvalho, J. E., Foglio, M. A., & Dolder, H. (2011) · BMC Complementary and Alternative Medicine · doi:10.1186/1472-6882-11-25 · Cited by 125 · see citing works
“They exert properties such as free radical scavenging and inhibiting the generation of reactive species [16,17].”
BackgroundChen, H., Lin, C.-M., Lee, C., et al. (2013) · Oncology Reports · doi:10.3892/or.2013.2490 · Cited by 124 · see citing works
The potential impact of strawberry on human health
Giampieri, F., Álvarez-Suárez, J. M., Mazzoni, L., Romandini, S., Bompadre, S., Diamanti, J., Capocasa, F., Mezzetti, B., Quiles, J. L., Ferreiro, M. S., et al. (2012) · Natural Product Research · doi:10.1080/14786419.2012.706294 · Cited by 123 · see citing works
“Strawberry extracts also seem to modulate cell signalling in cancer cells by inhibiting proliferation of several type of cancer cells (Zhang, Seeram, Lee, Feng, & Heber, 2008 ), inducing cell cycle arrest and apoptosis (Boivin, Blanchette, Barrette, Moghrabi, & Beliveau, 2007 ; Seeram et al, 2006 ), and suppressing tu…”
BackgroundBouyahya, A., Omari, N. E., Hachlafi, N. E., Jemli, M. E., Hakkour, M., Balahbib, A., Menyiy, N. E., Bakrim, S., Mrabti, H. N., Khouchlaa, A., et al. (2022) · Molecules · doi:10.3390/molecules27103286 · Cited by 122 · see citing works
Kaempferol, a new nutrition-derived pan-inhibitor of human histone deacetylases
Berger, A., Venturelli, S., Kallnischkies, M., Böcker, A., Busch, C., Weiland, T., Noor, S., Leischner, C., Weiß, T. S., Lauer, U. M., et al. (2012) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2012.07.001 · Cited by 116 · see citing works
“Furthermore, kaempferol is found as an ingredient in honey, some medical plants or green and black tea [7–12].”
BackgroundCyanidin-3-Glucoside inhibits ethanol-induced invasion of breast cancer cells overexpressing ErbB2
Xu, M. L., Bower, K. A., Wang, S., et al. (2010) · Molecular Cancer · doi:10.1186/1476-4598-9-285 · Cited by 115 · see citing works
“C3G exhibits anti-cancer properties in various in vitro and animal models of carcinogenesis and tumor development; the effects of C3G include the inhibition of tumor cell proliferation and the attenuation of cell migration/invasion as well as metastasis in vivo [13-18].”
Stanković, M. S., Milutinović, M. G., Žižić, J., Topuzović, M. D., Solujić, S., & Marković, S. (2011) · International Journal of Molecular Sciences · doi:10.3390/ijms12074190 · Cited by 110 · see citing works
“manner with different sensitivity between cell lines [24–27].”
BackgroundJeyaraj, E. J., Lim, Y. Y., & Choo, W. S. (2022) · Scientific Reports · doi:10.1038/s41598-022-19146-z · Cited by 109 · see citing works
Anticancer effects of bioactive berry compounds
Folmer, F., Basavaraju, U., Jaspars, M., Hold, G. L., El-Omar, E. M., Dicato, M. A., & Diederich, M. F. (2013) · Phytochemistry Reviews · doi:10.1007/s11101-013-9319-z · Cited by 112 · see citing works
“…and Shishodia 2006; Bae et al. 2009; Bagchi et al. 2004; Basu and Penugonda 2009; Boivin et al. 2007; Chen et al. 2006; Duthie 2007a; Gupta et al. 2010; Khan et al. 2007; Lu et al. 2006; Seeram 2008; Stoner et al. 2008; Toi et al. 2003; Wang et al. 2005; Zhang et al. 2008; Zikri et al. 2009).”
BackgroundInfluentialEllagitannins in Cancer Chemoprevention and Therapy
Ismail, T., Calcabrini, C., Diaz, A., Fimognari, C., Turrini, E., Catanzaro, E., Akhtar, S., & Sestili, P. (2016) · Toxins · doi:10.3390/toxins8050151 · Cited by 107 · see citing works
“, reported that strawberry crude extracts or their isolated components including ellagic acid were toxic toward human oral CAL-27 and KB tumor cells [109]; ellagic acid alone (50–200 μM) exhibited selective cytotoxicity against HSC-2 oral carcinoma cells [110].”
BackgroundCytotoxic constituents from the bark of Salix hulteni
Jeon, S. H., Chun, W., Choi, Y. J., et al. (2008) · Archives of Pharmacal Research · doi:10.1007/s12272-001-1255-9 · Cited by 104 · see citing works
Antioxidant and α-glucosidase inhibitory phenolics isolated from highbush blueberry flowers
Wan, C. C., Yuan, T., Cirello, A. L., et al. (2012) · Food Chemistry · doi:10.1016/j.foodchem.2012.06.056 · Cited by 101 · see citing works
Self-citationGiampieri, F., Álvarez-Suárez, J. M., Tulipani, S., Paramás, A. M. G., Santos‐Buelga, C., Bompadre, S., Quiles, J. L., Mezzetti, B., & Battino, M. A. (2012) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf205065x · Cited by 100 · see citing works
Yang, E. J., Kim, G., Jun, M., & Song, K. (2014) · Food & Function · doi:10.1039/c4fo00068d · Cited by 100 · see citing works
Lü, N., Bernardo, E. L., Tippayadarapanich, C., et al. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.00708 · Cited by 97 · see citing works
Salgado, J. M., Ferreira, T. R. B., Biazotto, F. D. O., & Dias, C. T. D. S. (2012) · Plant Foods for Human Nutrition · doi:10.1007/s11130-011-0264-y · Cited by 97 · see citing works
“In orange juice, vitamin C is the main antioxidant compound [35, 36], whereas strawberries contain anthocyanins, which are responsible for benefits to the immune system and prevent proliferation of human cancer cells originated from different body organs, such as the stomach, colon, breast, and the central nervous sys…”
BackgroundChauthe, S. K., Sharma, R. J., Aqil, F., et al. (2012) · Phytochemical Analysis · doi:10.1002/pca.2375 · Cited by 96 · see citing works
Andriani, Y., Ramli, N. M., Syamsumir, D. F., et al. (2015) · Arabian Journal of Chemistry · doi:10.1016/j.arabjc.2015.11.003 · Cited by 97 · see citing works
“, 2004) that include cancer (Zhang et al., 2008), antidiabetic (You et al.”
BackgroundAhmed, M., Fatima, H., Qasim, M., et al. (2017) · BMC Complementary and Alternative Medicine · doi:10.1186/s12906-017-1894-x · Cited by 94 · see citing works
“Anthocyanins, kaempferol, quercetin, esters of coumaric acid and ellagic acid were found to be inhibitors of human oral (KB, CAL-27), breast (MCF-7), colon (HT-29, HCT-116), and prostate (LNCaP, DU-145) tumor cell lines in a dose-dependent manner [44].”
BackgroundApplication of Anthocyanins from Blackcurrant ( Ribes nigrum L.) Fruit Waste as Renewable Hair Dyes
Rose, P. M., Cantrill, V., Benohoud, M., et al. (2018) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.8b01044 · Cited by 93 · see citing works
Koh, G. Y., Chou, G., & Liu, Z. (2009) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf900269r · Cited by 93 · see citing works
The effect of strawberries in a cholesterol-lowering dietary portfolio
Jenkins, D. J. A., Nguyen, T. H., Kendall, C. W. C., Faulkner, D. A., Bashyam, B., Kim, I. J., Ireland, C., Patel, D. K., Vidgen, E., Josse, A. R., et al. (2008) · Metabolism · doi:10.1016/j.metabol.2008.07.018 · Cited by 92 · see citing works
“In laboratory cell culture and animal studies, strawberry extracts and their antioxidant components have been shown to have anticancer properties [35-37].”
BackgroundPolyphenol‐rich beverages: insights from sensory and consumer science
Jaeger, S. R., Axten, L. G., Wohlers, M. W., & Sun‐Waterhouse, D. (2009) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.3721 · Cited by 92 · see citing works
Antiproliferative activities of tea and herbal infusions
Li, F., Li, S., Li, H., Deng, G., Ling, W., & Xu, X. (2013) · Food & Function · doi:10.1039/c2fo30252g · Cited by 86 · see citing works
Can phytochemical antioxidant rich foods act as anti-cancer agents?
Wang, S., Meckling, K. A., Marcone, M. F., Kakuda, Y., & Tsao, R. (2011) · Food Research International · doi:10.1016/j.foodres.2011.05.021 · Cited by 84 · see citing works
Sugata, M., Lin, C., & Shih, Y.-C. (2015) · BioMed Research International · doi:10.1155/2015/768093 · Cited by 83 · see citing works
Ghasemzadeh, A., Jaafar, H. Z. E., & Rahmat, A. B. (2015) · BMC Complementary and Alternative Medicine · doi:10.1186/s12906-015-0873-3 · Cited by 82 · see citing works
“For example, the isolated polyphenols from strawberry including kaempferol, quercetin, anthocyanins, coumaric acid and ellagic acid, were shown to inhibit the growth of human breast (MCF-7), oral (KB, CAL-27), colon (HT-29, HCT-116), and prostate (LNCaP, DU-145) tumor cell lines [13, 14].”
BackgroundEffect of BTH on antioxidant enzymes, radical-scavenging activity and decay in strawberry fruit
Cao, S., Hu, Z., Zheng, Y., et al. (2010) · Food Chemistry · doi:10.1016/j.foodchem.2010.08.051 · Cited by 80 · see citing works
Elucidating the role of polygalacturonase genes in strawberry fruit softening
Paniagua, C., Ric‐Varas, P., García-Gago, J. A., López‐Casado, G., Blanco‐Portales, R., Muñoz‐Blanco, J., Schückel, J., Knox, J. P., Matas, A. J., Quesada, M. A., et al. (2020) · Journal of Experimental Botany · doi:10.1093/jxb/eraa398 · Cited by 78 · see citing works
Mushtaq, M., Sultana, B., Akram, S., et al. (2017) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-017-0309-7 · Cited by 79 · see citing works
“In order to provide reliable data on antioxidant quality of extract, the researchers mostly assessed free radical scavenging capacity (RSC), reducing power (RP), total antioxidant equivalents (comparative to standard antioxidant Trolox or ascorbic acid), and inhibition of peroxidation in the linoleic acid system [37,…”
BackgroundCRISPR/Cas9 editing of the polygalacturonaseFaPG1gene improves strawberry fruit firmness
López‐Casado, G., Sánchez-Raya, C., Ric‐Varas, P., et al. (2023) · Horticulture Research · doi:10.1093/hr/uhad011 · Cited by 78 · see citing works
“Additionally, this fruit is relevant for its nutritional properties and beneficial effects on health [1].”
BackgroundRaudonis, R., Raudonė, L., Jakštas, V., & Janulis, V. (2012) · Journal of Chromatography A · doi:10.1016/j.chroma.2012.02.019 · Cited by 77 · see citing works
Hasibuan, P. A. Z., Harahap, U., Sitorus, P., et al. (2020) · Heliyon · doi:10.1016/j.heliyon.2020.e04449 · Cited by 74 · see citing works
“Cells that underwent apoptosis, a programmed cell death process, have altered morphology, membrane blebbing, and chromatin changes [39, 40].”
BackgroundGasmi, J., & Sanderson, J. T. (2010) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf103306k · Cited by 75 · see citing works
Song, C., Gu, L., Liu, J., et al. (2015) · Plant and Cell Physiology · doi:10.1093/pcp/pcv151 · Cited by 71 · see citing works
“By comparison of their MS and retention times with those of authentic references, compound 6 was identified as kaempferol-3-(6'-coumaroyl) glucoside (a product of kaempferol 3-glucoside) as a decreased metabolite in UGT71W2-RNAi line (Zhang et al. 2008).”
The Antioxidant and Anticancer Effects of Wild Carrot Oil Extract
Shebaby, W. N., El‐Sibai, M., Bodman-Smith, K. B., Karam, M., Mroueh, M. A., & Daher, C. F. (2012) · Phytotherapy Research · doi:10.1002/ptr.4776 · Cited by 69 · see citing works
“Several studies on phenols and polyphenols isolated from different plant food such as blackberry, cranberry, raspberry and strawberry were shown to inhibit the growth of several human cancer cell lines, including breast (MCF-7) and colon (HT-29), in a dose-dependent manner with different sensitivity between cells (See…”
BackgroundPolyphenols in Strawberry (Fragaria × ananassa) Leaves Induced by Plant Activators
Kårlund, A., Salminen, J., Koskinen, P., et al. (2014) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf405589f · Cited by 69 · see citing works
Young apple polyphenols postpone starch digestion in vitro and in vivo
Li, D., Sun, L., Yang, Y., Wang, Z., Yang, X., Zhao, T., Gong, T., Zou, L. J., & Guo, Y. (2019) · Journal of Functional Foods · doi:10.1016/j.jff.2019.03.009 · Cited by 71 · see citing works
Jeong, J. C., Jang, S. W., Kim, T. H., et al. (2010) · Nutrition and Cancer · doi:10.1080/01635580903441287 · Cited by 68 · see citing works
“Phenolic compounds isolated from berries and crude extracts inhibited the growth of human oral, colon, and prostate cancer cells ( 11 , 12 , 13 ).”
BackgroundKamonpatana, K., Failla, M. L., Kumar, P., & Giusti, M. M. (2014) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf405180k · Cited by 68 · see citing works
Phytochemical study of polyphenols in Perilla Frutescens as an antioxidant.
Asif, M. (2012) · Europe PMC (PubMed Central) · Cited by 67 · see citing works
Megdiche-Ksouri, W., Medini, F., Hammi, K. M., et al. (2013) · Food Chemistry · doi:10.1016/j.foodchem.2013.01.047 · Cited by 67 · see citing works
Somavat, P., Kumar, D., & Singh, V. (2018) · Industrial Crops and Products · doi:10.1016/j.indcrop.2018.02.015 · Cited by 66 · see citing works
Giampieri, F., Forbes-Hernández, T. Y., Gasparrini, M., Afrin, S., Cianciosi, D., Reboredo‐Rodríguez, P., Varela‐López, A., Quiles, J. L., Mezzetti, B., & Battino, M. A. (2017) · Annals of the New York Academy of Sciences · doi:10.1111/nyas.13373 · Cited by 65 · see citing works
“neuronal stress following exposure to (56)Fe particles.”
Quantitative and Fingerprint Analyses of Chinese Sweet Tea Plant (Rubus suavissimus S. Lee)
Chou, G., Xu, S., Liu, D., Koh, G. Y., Zhang, J., & Liu, Z. (2009) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf8029397 · Cited by 65 · see citing works
Liu, M.-P., Liao, M., Dai, C., Chen, J.-F., Yang, C., Liu, M., Chen, Z., & Yao, M. (2016) · Scientific Reports · doi:10.1038/srep34245 · Cited by 65 · see citing works
Phenolics from strawberry cv. Falandi and their antioxidant and α-glucosidase inhibitory activities
Yang, D., Xie, H., Jiang, Y., & Wei, X. (2015) · Food Chemistry · doi:10.1016/j.foodchem.2015.08.091 · Cited by 63 · see citing works
Jang, H. J., Ha, U., Kim, S., Yoon, B.-I., Han, D.-S., Yuk, S. M., & Kim, S.-W. (2010) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf102688g · Cited by 63 · see citing works
Health Benefits of Phenolic Compounds Against Cancers
Basli, A., Belkacem, N., & Amrani, I. (2017) · InTech eBooks · doi:10.5772/67232 · Cited by 68 · see citing works
Mottaghipisheh, J., Doustimotlagh, A. H., Irajie, C., et al. (2022) · Cells · doi:10.3390/cells11071070 · Cited by 62 · see citing works
“) LNCaP Crude extracts (250 μg/mL) and pure compounds including C3G, pelargonidin, pelargonidin-3-glucoside, and pelargonidin-3-rutinoside (100 μg/mL) inhibited the growth of tested cancer cells significantly [39]”
BackgroundInfluentialRadiolabelled cyanidin 3-O-glucoside is poorly absorbed in the mouse
Felgines, C., Krisa, S., Mauray, A., et al. (2010) · British Journal Of Nutrition · doi:10.1017/s0007114510000061 · Cited by 62 · see citing works
Plant-derived and dietary phenolic cinnamic acid derivatives: Anti-inflammatory properties
Freitas, M., Ribeiro, D., Janela, J. L. S., Varela, C. L., Costa, S. C., da Silva, E. J. T., Fernandes, E. G. R., & Roleira, F. M. F. (2024) · Food Chemistry · doi:10.1016/j.foodchem.2024.140080 · Cited by 69 · see citing works
Araújo, M. G. D. F., Hilário, F., Vilegas, W., et al. (2012) · International Journal of Molecular Sciences · doi:10.3390/ijms13079260 · Cited by 60 · see citing works
“Several studies have shown that phenolics are the bioactive compounds that offer more benefits to human health and many authors have reported a direct relationship between total phenolic content and antioxidant activity in numerous seeds, fruits and vegetables [18–20].”
BackgroundAnthocyanins in Colorectal Cancer Prevention Review
Shi, N., Chen, X. L., & Chen, T. (2021) · Antioxidants · doi:10.3390/antiox10101600 · Cited by 59 · see citing works
“Pure anthocyanin compounds differed in their efficacy on cell proliferation [77]”
BackgroundInfluentialTissue distribution of anthocyanins in rats fed a blackberry anthocyanin‐enriched diet
Felgines, C., Texier, O., Garcin, P., Besson, C., Lamaison, J., & Scalbert, A. (2009) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.200800323 · Cited by 59 · see citing works
Selective bio‐availability of phenolic acids from Scottish strawberries
Russell, W. R., Scobbie, L., Labat, A., & Duthie, G. G. (2009) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.200800302 · Cited by 57 · see citing works
Framing the perfect strawberry: An exercise in consumer-assisted selection of fruit crops
Colquhoun, T. A., Levin, L. A., MOSKOWITZ, H. R., Whitaker, V. M., Clark, D. G., & Folta, K. M. (2012) · Journal of Berry Research · doi:10.3233/jbr-2011-027 · Cited by 65 · see citing works
“Multiple lines of in vitro, animal or clinical evidence indicate that strawberry-prevalent compounds have potential health benefits such as cellular anti-proliferative activities [3, 4], lipoprotein control and cardio-protective effects [5, 6], maintenance of blood glucose levels [7], and prevention of specific digest…”
Coproduct yield comparisons of purple, blue and yellow dent corn for various milling processes
Somavat, P., Li, Q., de Mejı́a, E. G., Liu, W., & Singh, V. (2016) · Industrial Crops and Products · doi:10.1016/j.indcrop.2016.04.062 · Cited by 57 · see citing works
- Authors
- Zhang, Y., Seeram, N. P., Lee, R., Feng, L., & Heber, D.
- Affiliations
- University of California, Los Angeles (US)
- Corresponding author
- Seeram, Navindra P.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2008-02
- Volume
- 56
- Issue
- 3
- Pages
- 670-675
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2021635663
- Cited by
- 365
- References
- 0
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 14 · 2023: 13 · 2022: 14 · 2021: 13 · 2020: 22 · 2019: 17 · 2018: 27 · 2017: 24 · 2016: 23 · 2015: 27 · 2014: 27 · 2013: 32 · 2012: 35
- FWCI
- 13.32
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Berry genetics and cultivation research
- Health Sciences › Nursing › Nutrition and Dietetics › Pomegranate: compositions and health benefits
- Keywords
- strawberry, anthocyanins, cyanidin-3-glucoside, pelargonidin, kaempferol, quercetin, ellagic acid, antioxidant activity, antiproliferative activity, cancer cell lines, oral cancer, colon cancer, prostate cancer
- MeSH terms
- Antineoplastic Agents, Phytogenic, Antioxidants, Cell Division, Fruit, Humans, Phenols, Fragaria, Cell Line, Tumor
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
