Protective effects of Chinese propolis and its component, chrysin, against neuronal cell death via inhibition of mitochondrial apoptosis pathway in SH-SY5Y cells
Abstract
Endoplasmic reticulum (ER) stress has been implicated in the pathogenesis of neurodegenerative and ischemic disorders. The purpose of this study was to evaluate the effects of Chinese propolis and its constituents [chrysin, galangin, pinocembrin, caffeic acid, and caffeic acid phenethyl ester (CAPE)] against tunicamycin-induced neuronal cell death in SH-SY5Y cells. Both Chinese propolis and chrysin concentration-dependently inhibited such cell death, the tunicamycin-induced activation of caspase-3, and the effects of tunicamycin on mitochondria [release of cytochrome c into the cytosol and disruption of the mitochondrial membrane potential (DeltaPsim)]. Furthermore, Chinese propolis and chrysin each inhibited staurosporine-induced cell death. These findings indicate that the inhibitory effects of Chinese propolis against neuronal cell death induced by ER stress or staurosporine may be exerted primarily by chrysin. Moreover, the mechanism underlying the protective effects may, at least partly, involve inhibitions of caspase-3 activity and the mitochondrial apoptotic pathway.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The human neuroblastoma SH-SY5Y is among the most used cell lines, a subclone of the parental neuronal line SK-N-SH, which originated in 1970 from a biopsy metastatic tumor.
“The human neuroblastoma SH-SY5Y is among the most used cell lines [131,136,137,138,139,140,141,142], a subclone of a parental neuronal line SK-N-SH, which originated in 1970 from a biopsy metastatic tumor.”
From Multi-Target Mechanisms of Phytochemicals in Alzheimer’s Disease: Effects on Oxidative Stress, Neuroinflammation and Protein Aggregation (2022) · cites this paper as [136]
Chinese propolis components inhibited tunicamycin-induced neuronal death in SH-SY5Y cells and decreased induced caspase 3 activation and mitochondrial membrane potential disruption.
“The authors reported the inhibition of neuronal death, and that propolis decreased the induced activation of caspase 3 and the effects on mitochondrial membrane potential disruption [136].”
From Multi-Target Mechanisms of Phytochemicals in Alzheimer’s Disease: Effects on Oxidative Stress, Neuroinflammation and Protein Aggregation (2022) · cites this paper as [136]
Cites
Kaufman, R. J. (1999) · Genes & Development · doi:10.1101/gad.13.10.1211 · Cited by 2,247 · see citing works
Coupling endoplasmic reticulum stress to the cell death program
Rao, R. V., Ellerby, H. M., & Bredesen, D. E. (2004) · Cell Death and Differentiation · doi:10.1038/sj.cdd.4401378 · Cited by 1,208 · see citing works
Effect of Plant Flavonoids on Immune and Inflammatory Cell Function
Middleton, E. (1998) · Advances in experimental medicine and biology · doi:10.1007/978-1-4615-5335-9_13 · Cited by 735 · see citing works
Signals from the Stressed Endoplasmic Reticulum Induce C/EBP-Homologous Protein (CHOP/GADD153)
Wang, X.-Z., Lawson, B., Brewer, J. W., Zinszner, H., Sanjay, A., Mi, L.-J., Boorstein, R. J., Kreibich, G., Hendershot, L. M., & Ron, D. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.8.4273 · Cited by 669 · see citing works
Deniaud, A., Sharaf el dein, O., Maillier, E., Poncet, D., Kroemer, G., Lemaire, C., & Brenner, C. (2008) · Oncogene · doi:10.1038/sj.onc.1210638 · Cited by 586 · see citing works
The Mitochondrial Death Pathway and Cardiac Myocyte Apoptosis
Crow, M. T., Mani, K. M., Nam, Y., & Kitsis, R. N. (2004) · Circulation Research · doi:10.1161/01.res.0000148632.35500.d9 · Cited by 584 · see citing works
Arora, A., Nair, M. G., & Strasburg, G. M. (1998) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(97)00458-9 · Cited by 562 · see citing works
Endoplasmic reticulum stress-mediated apoptosis in pancreatic β-cells
Oyadomari, S., Araki, E., & Mori, M. (2002) · APOPTOSIS · doi:10.1023/a:1016175429877 · Cited by 517 · see citing works
Gene expression during ER stress–induced apoptosis in neurons
Reimertz, C., Kögel, D., Rami, A., Chittenden, T., & Prehn, J. H. M. (2003) · The Journal of Cell Biology · doi:10.1083/jcb.200305149 · Cited by 367 · see citing works
The effect of propolis and its components on eicosanoid production during the inflammatory response
Mirzoeva, O. K., & Calder, P. C. (1996) · Prostaglandins Leukotrienes and Essential Fatty Acids · doi:10.1016/s0952-3278(96)90129-5 · Cited by 295 · see citing works
Shkoda, A., Ruiz, P. A., Daniel, H., Kim, S. C., Rogler, G., Sartor, R. B., & Haller, D. (2006) · Gastroenterology · doi:10.1053/j.gastro.2006.10.030 · Cited by 278 · see citing works
Modulation of the activity of pro-inflammatory enzymes, COX-2 and iNOS, by chrysin derivatives
Cho, H. (2003) · Pharmacological Research · doi:10.1016/s1043-6618(03)00248-2 · Cited by 249 · see citing works
Gardana, C., Scaglianti, M., Pietta, P., et al. (2007) · Journal of Pharmaceutical and Biomedical Analysis · doi:10.1016/j.jpba.2007.06.022 · Cited by 247 · see citing works
Cárdenas, M., Marder, M., Blank, V. C., & Roguin, L. P. (2006) · Bioorganic & Medicinal Chemistry · doi:10.1016/j.bmc.2005.12.021 · Cited by 247 · see citing works
Mercer, L. D., Kelly, B. L., Horne, M. K., & Beart, P. M. (2004) · Biochemical Pharmacology · doi:10.1016/j.bcp.2004.09.018 · Cited by 241 · see citing works
Nakajima, Y., Shimazawa, M., Mishima, S., & Hara, H. (2006) · Life Sciences · doi:10.1016/j.lfs.2006.09.017 · Cited by 235 · see citing works
Self-citationEndo, M., Mori, M., Akira, S., & Gotoh, T. (2006) · The Journal of Immunology · doi:10.4049/jimmunol.176.10.6245 · Cited by 221 · see citing works
Arnoult, D., Akarid, K., Grodet, A., PETIT, P. X., Estaquier, J., & Ameisen, J. (2002) · Cell Death and Differentiation · doi:10.1038/sj.cdd.4400951 · Cited by 218 · see citing works
Biological activities of the two major components of tunicamycin.
Mahoney, W. C., & Duksin, D. (1979) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)50406-3 · Cited by 203 · see citing works
Basnet, P., Matsushige, K., Hase, K., Kadota, S., & Namba, T. (1996) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.19.1479 · Cited by 201 · see citing works
Antioxidant and Prooxidant Effects of Phenolics on Pancreatic β-Cells in Vitro
Lapidot, T., WALKER, M. D., & Kanner, J. (2002) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf020615a · Cited by 196 · see citing works
Inhibition of HIV Activation in Latently Infected Cells by Flavonoid Compounds
Critchfield, J. W., Butera, S. T., & Folks, T. M. (1996) · AIDS Research and Human Retroviruses · doi:10.1089/aid.1996.12.39 · Cited by 179 · see citing works
Antibacterial Diterpenic Acids from Brazilian Propolis
Bankova, V. S., Marcucci, M. C., Simova, S., et al. (1996) · Zeitschrift für Naturforschung C · doi:10.1515/znc-1996-5-602 · Cited by 168 · see citing works
Chen, C.-N., Weng, M., Wu, C., & Lin, J. (2004) · Evidence-based Complementary and Alternative Medicine · doi:10.1093/ecam/neh034 · Cited by 159 · see citing works
Analysis of flavonoids from propolis by on-line HPLC–electrospray mass spectrometry
Volpi, N., & Bergonzini, G. (2006) · Journal of Pharmaceutical and Biomedical Analysis · doi:10.1016/j.jpba.2006.04.017 · Cited by 159 · see citing works
Chrysin and other leaf exudate flavonoids in the genus Pelargonium
Williams, C. A., Harborne, J. B., Newman, M., et al. (1997) · Phytochemistry · doi:10.1016/s0031-9422(97)00514-1 · Cited by 115 · see citing works
Free Radical Scavenging by Ethanol Extract of Propolis
Scheller, S., Wilczok, T., Imielski, S., et al. (1990) · International Journal of Radiation Biology · doi:10.1080/09553009014552601 · Cited by 113 · see citing works
Endoplasmic reticulum signaling as a determinant of recombinant protein expression
Cudna, R. E., & Dickson, A. J. (2002) · Biotechnology and Bioengineering · doi:10.1002/bit.10445 · Cited by 97 · see citing works
Yamazaki, T., Muramoto, M., Oe, T., et al. (2006) · Neuropharmacology · doi:10.1016/j.neuropharm.2005.10.016 · Cited by 53 · see citing works
Oda, T., Kosuge, Y., Arakawa, M., et al. (2007) · Neuroscience Research · doi:10.1016/j.neures.2007.09.005 · Cited by 51 · see citing works
Muramatsu, Y., Maemoto, T., Iwashita, A., & Matsuoka, N. (2007) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2007.01.060 · Cited by 15 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Sharifi-Rad, J., Rapposelli, S., Sestito, S., Herrera-Bravo, J., Arancibia-Diaz, A., Salazar, L. A., Yeskaliyeva, B., Beyatli, A., Leyva-Gómez, G., González-Contreras, C., et al. (2022) · Journal of Personalized Medicine · doi:10.3390/jpm12091515 · Cited by 125 · see citing works
- Authors
- Izuta, H., Shimazawa, M., Tazawa, S., Araki, Y., Mishima, S., & Hara, H.
- Affiliations
- Gifu Pharmaceutical University (JP)
- Corresponding author
- Hara, Hideaki
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2008-09-12
- Volume
- 56
- Issue
- 19
- Pages
- 8944-8953
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2070668428
- Cited by
- 93
- References
- 31
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 4 · 2023: 7 · 2022: 2 · 2021: 8 · 2020: 11 · 2019: 3 · 2018: 3 · 2017: 4 · 2016: 10 · 2015: 6 · 2014: 4 · 2013: 3 · 2012: 5
- FWCI
- 6.04
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Bee Products Chemical Analysis
- Health Sciences › Medicine › Pharmacology › Flavonoids in Medical Research
- Health Sciences › Medicine › Pharmacology › Healthcare and Venom Research
- Keywords
- Chinese propolis, chrysin, neuronal cell death, endoplasmic reticulum stress, mitochondrial apoptosis, caspase-3, cytochrome c release, mitochondrial membrane potential, tunicamycin, staurosporine, SH-SY5Y cells, neuroprotection, caffeic acid phenethyl ester, galangin, pinocembrin, caffeic acid
- MeSH terms
- Endoplasmic Reticulum, Enzyme Activation, Flavonoids, Humans, Mitochondria, Neuroblastoma, Neurons, Propolis, Tunicamycin, Apoptosis, Staurosporine, Cell Line, Tumor, Membrane Potential, Mitochondrial, Caspase 3
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
