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Novel Anticancer Activity of the Autocleaved Ovotransferrin against Human Colon and Breast Cancer Cells
Abstract
Proteins of avian egg albumin have been suggested to play various biological roles during the development of chick embryo to confer protection. Recently, we have shown that ovotransferrin (OTf), the second major protein in egg albumin, undergoes thiol-linked autocleavage at distinct sites upon reduction. This study explores the physiological significance of OTf autocleavage by examining the effect of the reduced autocleaved OTf (termed rac-OTf) on modulation of cell proliferation, lethality, and apoptosis in two human cancer cell lines, colon cancer (HCT-116) and breast cancer (MCF-7). The rac-OTf was prepared by reduction of OTf with a non-thiol reductant (TCEP), to avoid reductive alkylation and produce highly soluble fragments. Unlike OTf, rac-OTf remarkably inhibited the proliferation of cancerous MCF-7 and HCT-116 cells in a dose-dependent manner, with the greatest effect on HCT-116, but had no effect on normal human mammary epithelial cells (HMEC). Cytofluorometric and trypan blue exclusion analyses indicated that rac-OTf exhibits cytotoxicity to HCT-116 in a dose-dependent fashion. The cytotoxic mechanism of rac-OTf against cancer cells was found to be induction of apoptosis as judged by changes in cell morphology, annexin-V binding, collapse of mitochondrial membrane potential, and caspase-9 and -6 activation, indicating the involvement of the mitochondrial pathway. This finding is the first to describe the reduction-dependent autocleaved OTf as an anticancer molecule…
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Partially hydrolyzed products of ovotransferrin have very strong anticancer effects against colon and breast cancer cells.
“Ibrahim and Kiyono (2009) reported that ovotransferrin underwent thiol-linked auto-cleavage after reduction, and produced partially hydrolyzed products with very strong anticancer effects against colon and breast cancer cells.”
From Egg white proteins and their potential use in food processing or as nutraceutical and pharmaceutical agents—A review (2013) · cites this paper as Ibrahim and Kiyono, 2009
Ovotransferrin undergoes thiol-linked auto-cleavage after reduction.
“Ibrahim and Kiyono (2009) reported that ovotransferrin underwent thiol-linked auto-cleavage after reduction, and produced partially hydrolyzed products with very strong anticancer effects against colon and breast cancer cells.”
From Egg white proteins and their potential use in food processing or as nutraceutical and pharmaceutical agents—A review (2013) · cites this paper as Ibrahim and Kiyono, 2009
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Metadata
- Authors
- Ibrahim, H. R., & Kiyono, T.
- Affiliations
- Kagoshima University (JP)
- National Cancer Center Research Institute (JP)
- Corresponding author
- Ibrahim, Hisham R.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2009-12-09
- Volume
- 57
- Issue
- 23
- Pages
- 11383-11390
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2043270798
- Cited by
- 61
- References
- 0
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 4 · 2022: 6 · 2021: 5 · 2020: 4 · 2019: 7 · 2018: 6 · 2017: 4 · 2016: 2 · 2015: 3 · 2014: 5 · 2013: 5 · 2012: 3
- FWCI
- 0.67
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Cancer Research Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Life Sciences › Immunology and Microbiology › Immunology › Toxin Mechanisms and Immunotoxins
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Bioactive Compounds and Antitumor Agents
- Keywords
- ovotransferrin, autocleavage, ovalbumin, anticancer activity, colon cancer, breast cancer, apoptosis, mitochondrial apoptosis, caspase activation, cytotoxicity, cell proliferation inhibition, nutraceuticals, TCEP reduction
- MeSH terms
- Animals, Antineoplastic Agents, Breast Neoplasms, Chickens, Colonic Neoplasms, Conalbumin, Humans, Apoptosis, HCT116 Cells, Cell Line, Tumor, Cell Proliferation
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