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Comparison of Oxidative Processes on Myofibrillar Proteins from Beef during Maturation and by Different Model Oxidation Systems

Martinaud, Agnès, Mercier, Yves, Marinova, Penka, Tassy, Caroline, Gatellier, Philippe, Renerre, Michel·1997·Journal of Agricultural and Food Chemistry

Abstract

The aim of this work was to compare the oxidative processes occurring in myofibrillar proteins during meat maturation and after an in vitro exposure to different enzymic and nonenzymic oxidative systems. Myofibrils were prepared from bovine Longissimus lumborum and Diaphragma pedialis at day 1 and day 10 post-mortem. Myofibrillar protein oxidation was measured by the carbonyl content, with the 2,4-dinitrophenylhydrazine (DNPH) method, and by (thiol group) SH content with the 2,2‘-dithiobis(5-nitropyridine) (DTNP) method. Polymerization and/or fragmentation of oxidized proteins were estimated by SDS-PAGE and Western blot analysis using a polyclonal antibody to myosin. Oxidation of myofibrillar proteins is dependent upon the different metal-catalyzed oxidation (MCO) systems. The increase in carbonyl content and also the decrease in SH content of myofibrillar proteins, after maturation of 10 days, were similar to those obtained after a 1 h incubation of myofibrillar proteins in the presence of several MCO systems. Electrophoretic studies showed that myosin was the protein the most sensitive to oxidation, and to a lesser extent, troponin T. Myosin-oxidative products were also detected by Western blot analysis. Keywords: Beef; myofibrillar proteins; protein oxidation; carbonyl content; SH-content; metal-ion catalyzed oxidation system; SDS−PAGE; Western blotting

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Metadata

Authors
Martinaud, A., Mercier, Y., Marinova, P., Tassy, C., Gatellier, P., & Renerre, M.
Venue
Journal of Agricultural and Food Chemistry
Published
1997-07-01
Volume
45
Issue
7
Pages
2481-2487
ISSN
  • 0021-8561 (ISSN-L)
  • 1520-5118
OpenAlex
W2014641141
Cited by
239
References
0
FWCI
3.27
Type
journal article
Language
en
Open access
closed

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Martinaud, A., Mercier, Y., Marinova, P., Tassy, C., Gatellier, P., & Renerre, M. (1997). Comparison of Oxidative Processes on Myofibrillar Proteins from Beef during Maturation and by Different Model Oxidation Systems. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/jf960977g

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