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Interactions of Grape Seed Tannins with Salivary Proteins

Sarni-Manchado, Pascale, Cheynier, Véronique, Moutounet, Michel·1999·Journal of Agricultural and Food Chemistry

Abstract

To evaluate the amount and type of condensed tannins binding salivary proteins, which are supposed to be involved in astringent sensation, model systems allowing further analyses of proteins and condensed tannins were developed. The precipitates formed after addition of grape seed tannins to salivary proteins indicate that a binding interaction occurs. Dissociation of insoluble complexes was achieved by sodium dodecyl sulfate treatment. Thiolysis reaction allowed the quantification and characterization of proanthocyanidins on both the resulting pellet and the supernatant. Binding proteins were investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The higher polymerized tannins predominantly precipitated together with the salivary proteins. The condensed tannins remaining in solution were low molecular weight polymers.

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Metadata

Authors
Sarni-Manchado, P., Cheynier, V., & Moutounet, M.
Venue
Journal of Agricultural and Food Chemistry
Published
1998-12-08
Volume
47
Issue
1
Pages
42-47
ISSN
  • 0021-8561 (ISSN-L)
  • 1520-5118
OpenAlex
W2038536576
Cited by
248
References
0
FWCI
2.40
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Interactions of Grape Seed Tannins with Salivary Proteins. https://app.cochat.ai/papers/10.1021/jf9805146
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Sarni-Manchado, P., Cheynier, V., & Moutounet, M. (1999). Interactions of Grape Seed Tannins with Salivary Proteins. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/jf9805146

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

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