The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Electrospray and Tandem Mass Spectroscopy As Tools for Anthocyanin Characterization
Abstract
The utility of electrospray and tandem mass spectroscopy (ES-MS and MS-MS) in anthocyanin characterization was tested using different anthocyanin extracts. Anthocyanins were semipurified by using a C-18 resin, washed with acidified water followed by ethyl acetate, and recovered with acidified methanol. Samples were directly injected into a mass spectrometer in either aqueous or methanolic solutions. The positive charge of anthocyanins favored fast and effective ES-MS detection of intact molecular ions. Little interference from other compounds was observed when the ethyl acetate cleaning procedure was used. Tandem mass spectroscopy provided clear and characteristic fragmentation patterns. The voltage used affected only the proportions at which these fragments were present. ES-MS may be used as a fast procedure for identification of anthocyanins, requiring minimal sample preparation. In combination with HPLC, ES-MS and MS-MS could be very powerful tools for anthocyanin characterization and monitoring the authenticity of anthocyanin-containing fruit juices and vegetable extracts.
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Citations
Cites
Electrospray Ionization for Mass Spectrometry of Large Biomolecules
Fenn, J. B., Mann, M., Meng, C. K., Wong, S. F., & Whitehouse, C. M. (1989) · Science · doi:10.1126/science.2675315 · Cited by 7,634
Cited by
Acylated anthocyanins from edible sources and their applications in food systems
Giusti, M. M., & Wrolstad, R. E. (2003) · Biochemical Engineering Journal · doi:10.1016/s1369-703x(02)00221-8 · Cited by 954
Self-citationTracking color and pigment changes in anthocyanin products
Wrolstad, R. E., Durst, R. W., & Lee, J. (2005) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2005.03.019 · Cited by 782
Self-citationCho, M. J., Howard, L. R., Prior, R. L., & Clark, J. R. (2004) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.1885 · Cited by 398
Ju, Z. Y., & Howard, L. R. (2003) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf0302106 · Cited by 388
Thermal degradation of anthocyanins and its impact on color and in vitro antioxidant capacity
Sadilova, E., Carle, R., & Stintzing, F. C. (2007) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.200700179 · Cited by 385
Metadata
- Authors
- Giusti, M. M., Rodríguez-Saona, L. E., Griffin, D., & Wrolstad, R. E.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1999-10-12
- Volume
- 47
- Issue
- 11
- Pages
- 4657-4664
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W1990832325
- Cited by
- 257
- References
- 1
- FWCI
- 1.07
- Type
- journal article
- Language
- en
- Open access
- closed
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