The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Silica Nanoparticle Size Influences the Structure and Enzymatic Activity of Adsorbed Lysozyme
Abstract
Adsorption of chicken egg lysozyme on silica nanoparticles of various diameters has been studied. Special attention has been paid to the effect of nanoparticle size on the structure and function of the adsorbed protein molecules. Both adsorption patterns and protein structure and function are strongly dependent on the size of the nanoparticles. Formation of molecular complexes is observed for adsorption onto 4-nm silica. True adsorptive behavior is evident on 20- and 100-nm particles, with the former resulting in monolayer adsorption and the latter yielding multilayer adsorption. A decrease in the solution pH results in a decrease in lysozyme adsorption. A change of protein structure upon adsorption is observed, as characterized by a loss in alpha-helix content, and this is strongly dependent on the size of the nanoparticle and the solution pH. Generally, greater loss of alpha helicity was observed for the lysozyme adsorbed onto larger nanoparticles under otherwise similar conditions. The activity of lysozyme adsorbed onto silica nanoparticles is lower than that of the free protein, and the fraction of activity lost correlates well with the decrease in alpha-helix content. These results indicate that the size of the nanoparticle, perhaps because of the contributions of surface curvature, influences adsorbed protein structure and function.
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Cites
Noncovalent sidewall functionalization of single-walled carbon nanotubes for protein immobilization
Chen, R. J., Zhang, Y., Wang, D., & Dai, H. (2001) · Journal of the American Chemical Society · doi:10.1021/ja010172b · Cited by 2,534
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Nel, A., Xia, T., Mädler, L., & Li, N. (2006) · Science · doi:10.1126/science.1114397 · Cited by 9,324
Understanding biophysicochemical interactions at the nano–bio interface
Nel, A. E., Mädler, L., Velegol, D., Xia, T., Hoek, E. M. V., Somasundaran, P., Klaessig, F., Castranova, V., & Thompson, M. (2009) · Nature Materials · doi:10.1038/nmat2442 · Cited by 7,083
Aggarwal, P., Hall, J. B., McLeland, C. B., Dobrovolskaia, M. A., & McNeil, S. E. (2009) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2009.03.009 · Cited by 1,791
Walkey, C. D., & Chan, W. C. W. (2012) · Chem. Soc. Rev. · doi:10.1039/c1cs15233e · Cited by 1,758
Protein−Nanoparticle Interactions: Opportunities and Challenges
Mahmoudi, M., Lynch, I., Ejtehadi, M. R., Monopoli, M. P., Bombelli, F. B., & Laurent, S. (2011) · Chemical Reviews · doi:10.1021/cr100440g · Cited by 1,426
Metadata
- Authors
- Vertegel, A. A., Siegel, R. W., & Dordick, J. S.
- Venue
- Langmuir
- Publisher
- American Chemical Society (ACS)
- Published
- 2004-05-27
- Volume
- 20
- Issue
- 16
- Pages
- 6800-6807
- ISSN
- 0743-7463 (ISSN-L)
- 1520-5827
- OpenAlex
- W1969814828
- Cited by
- 878
- References
- 1
- FWCI
- 9.42
- Type
- journal article
- Language
- en
- Open access
- closed
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