The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Structure, Function, and Stability of Enzymes Covalently Attached to Single-Walled Carbon Nanotubes
Abstract
We describe the structure, activity, and stability of enzymes covalently attached to single-walled carbon nanotubes (SWNTs). Conjugates of SWNTs with three functionally unrelated enzymes-horseradish peroxidase, subtilisin Carlsberg, and chicken egg white lysozyme-were found to be soluble in aqueous solutions. Furthermore, characterization of the secondary and tertiary structure of the immobilized proteins by circular dichroism and fluorescence spectroscopies, respectively, and determination of enzyme kinetics revealed that the enzymes retained a high fraction of their native structure and activity upon attachment to SWNTs. The SWNT-enzyme conjugates were also more stable in guanidine hydrochloride (GdnHCl) and at elevated temperatures relative to their solution counterparts. Thus, these protein conjugates represent novel preparations that possess the attributes of both soluble enzymes-high activity and low diffusional resistance-and immobilized enzymes-high stability-making them attractive choices for applications ranging from diagnostics and sensing to drug delivery.
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Cites
Enzyme-coated carbon nanotubes as single-molecule biosensors
Besteman, K., Lee, J.-O., Wiertz, F. G. M., Heering, H. A., & Dekker, C. (2003) · Nano Letters · doi:10.1021/nl034139u · Cited by 1,294
Cited by
Sapsford, K. E., Tyner, K. M., Dair, B. J., Deschamps, J. R., & Medintz, I. L. (2011) · Analytical Chemistry · doi:10.1021/ac200853a · Cited by 538
Cell-penetrating and cell-targeting peptides in drug delivery
Vivès, E., Schmidt, J., & Pèlegrin, A. (2008) · Biochimica et Biophysica Acta (BBA) - Reviews on Cancer · doi:10.1016/j.bbcan.2008.03.001 · Cited by 441
Strategies of enzyme immobilization on nanomatrix supports and their intracellular delivery
Sharifi, M., Karim, A. Y., Mustafa Qadir Nanakali, N., Salihi, A., Aziz, F. M., Hong, J., Khan, R. H., Saboury, A. A., Hasan, A., Abou-Zied, O. K., et al. (2020) · Journal of Biomolecular Structure and Dynamics · doi:10.1080/07391102.2019.1643787 · Cited by 29
Metadata
- Authors
- Asuri, P., Bale, S. S., Pangule, R. C., Shah, D. A., Kane, R. S., & Dordick, J. S.
- Venue
- Langmuir
- Publisher
- American Chemical Society (ACS)
- Published
- 2007-10-18
- Volume
- 23
- Issue
- 24
- Pages
- 12318-12321
- ISSN
- 0743-7463 (ISSN-L)
- 1520-5827
- OpenAlex
- W2019873707
- Cited by
- 179
- References
- 1
- FWCI
- 4.79
- Type
- journal article
- Language
- en
- Open access
- closed
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